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Tytuł pozycji:

Adsorption mechanism of triterpenoid saponins in reversed-phase liquid chromatography and hydrophilic interaction liquid chromatography: Mogroside V as test substance.

Tytuł:
Adsorption mechanism of triterpenoid saponins in reversed-phase liquid chromatography and hydrophilic interaction liquid chromatography: Mogroside V as test substance.
Autorzy:
Fu Q; Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.
Ni L; Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.
Jiang D; Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.
Ke Y; Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: .
Jin Y; Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: .
Źródło:
Journal of chromatography. A [J Chromatogr A] 2020 Jun 07; Vol. 1620, pp. 461010. Date of Electronic Publication: 2020 Mar 06.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Original Publication: Amsterdam ; New York : Elsevier, 1993-
MeSH Terms:
Hydrophobic and Hydrophilic Interactions*
Chromatography, Liquid/*methods
Chromatography, Reverse-Phase/*methods
Saponins/*isolation & purification
Triterpenes/*isolation & purification
Adsorption ; Saponins/chemistry ; Solvents ; Temperature ; Triterpenes/chemistry
Contributed Indexing:
Keywords: Adsorption isotherm; Hydrophilic interaction liquid chromatography (HILIC); Mogroside V; Reversed-phase liquid chromatography (RPLC); Separation; Triterpenoid saponins
Substance Nomenclature:
0 (Saponins)
0 (Solvents)
0 (Triterpenes)
88901-36-4 (mogroside V)
Entry Date(s):
Date Created: 20200317 Date Completed: 20200707 Latest Revision: 20200707
Update Code:
20240105
DOI:
10.1016/j.chroma.2020.461010
PMID:
32173025
Czasopismo naukowe
In this paper, adsorption mechanism of triterpenoid saponins in reversed-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) was proposed based on the study of the retention behavior of mogroside V as test substance. The change of peak shape of mogroside V and its influencing factors was first investigated. As the increase of sample loading, a tailing peak of mogroside V was observed in MeOHH 2 O of both two modes. It was the fronting peak in ACNH 2 O of HILIC while there was a transition from fronting peak to tailing peak in ACNH 2 O of RPLC that was largely affected by column temperature and ACN concentration. The adsorption isotherm of mogroside V in ACNH 2 O of RPLC was fitted by Moreau model, where a monolayer adsorption with large inter-molecular interaction was formed on the C18 surface. While in ACNH 2 O of HILIC, the adsorption of mogroside V was in accordance with BET model, showing multilayer adsorption behavior. In MeOHH 2 O of both HILIC and RPLC, there was always monolayer adsorption, which was fitted by Langmuir model. At last, by choosing the suitable chromatographic mode, controlling the key factors such as the solvent concentration and column temperature, and predicting the broadening trend of peak, three methods were screened out, namely, C18 column with 22% ACN (30 °C), Click XIon column with 90% MeOH or 70% ACN, to get mogroside V of purity greater than 98% from Siraitia grosvenorii extract. Among them, the RPLC method of 22% ACN that showed the highest loading sample per hour (1.92%) and the lowest solvent consumption emerged as the best approach.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020. Published by Elsevier B.V.)

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