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Tytuł:
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High-purity isolation of rare single cells from blood using a tiered microchip system.
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Autorzy:
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Gur O; School of Electrical Engineering, Purdue University, West Lafayette, IN, United States of America.; Birck Nanotechnology Center, Purdue University, West Lafayette, IN, United States of America.
Chang CL; Birck Nanotechnology Center, Purdue University, West Lafayette, IN, United States of America.; School of Mechanical Engineering, Purdue University, West Lafayette, IN, United States of America.
Jain R; Birck Nanotechnology Center, Purdue University, West Lafayette, IN, United States of America.; School of Mechanical Engineering, Purdue University, West Lafayette, IN, United States of America.
Zhong Y; Birck Nanotechnology Center, Purdue University, West Lafayette, IN, United States of America.; School of Mechanical Engineering, Purdue University, West Lafayette, IN, United States of America.
Savran CA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN, United States of America.; School of Mechanical Engineering, Purdue University, West Lafayette, IN, United States of America.
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Źródło:
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PloS one [PLoS One] 2020 Mar 17; Vol. 15 (3), pp. e0229949. Date of Electronic Publication: 2020 Mar 17 (Print Publication: 2020).
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
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Język:
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English
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Imprint Name(s):
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Original Publication: San Francisco, CA : Public Library of Science
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MeSH Terms:
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Cell Separation*
Microchip Analytical Procedures*
Single-Cell Analysis*
Neoplasms/*blood
Cell Line, Tumor ; Humans ; Leukocytes/cytology ; Microarray Analysis ; Microfluidics/methods ; Neoplasms/pathology ; Trophoblasts/cytology
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Entry Date(s):
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Date Created: 20200318 Date Completed: 20200617 Latest Revision: 20240328
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Update Code:
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20240329
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PubMed Central ID:
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PMC7077832
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DOI:
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10.1371/journal.pone.0229949
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PMID:
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32182245
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We present a two-tiered microchip system to capture and retrieve rare cells from blood samples with high purity. The first module of the system is a high throughput microfluidic interface that is used to immunomagnetically isolate targeted rare cells from whole blood, and discard > 99.999% of the unwanted leukocytes. The second module is a microwell array that furthers the purification by magnetically guiding each cell into a separate well concurrently, and allows individual retrieval of each cell. We demonstrate the design of the system as well as its characterization by experiments using model cell lines that represent circulating fetal trophoblasts. Our results show that single cells can be retrieved with efficiencies and purities as high as 100% within 145 mins.
Competing Interests: I have read the journal's policy and the authors of this manuscript have the following competing interests: C.S. and C-L.C. are share holders of Savran Technologies Inc. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
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