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Tytuł:
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Let-7b-5p promotes electroacupuncture tolerance by downregulating Penk1 gene in CFA-induced inflammatory nociception rats.
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Autorzy:
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Zhang Q; College of Veterinary Medicine, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, Hubei 430070, PR China. Electronic address: .
Liu A; College of Veterinary Medicine, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, Hubei 430070, PR China. Electronic address: .
Abouelfetouh MM; College of Veterinary Medicine, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, Hubei 430070, PR China. Electronic address: .
Ma N; College of Veterinary Medicine, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, Hubei 430070, PR China.
Li M; College of Veterinary Medicine, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, Hubei 430070, PR China.
Chen S; College of Veterinary Medicine, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, Hubei 430070, PR China.
Ding M; College of Veterinary Medicine, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, Hubei 430070, PR China. Electronic address: .
Ding Y; College of Veterinary Medicine, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, Hubei 430070, PR China. Electronic address: .
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Źródło:
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Gene [Gene] 2020 Jun 05; Vol. 742, pp. 144583. Date of Electronic Publication: 2020 Mar 14.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Amsterdam, Elsevier/North-Holland, 1976-
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MeSH Terms:
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Electroacupuncture*
Enkephalins/*genetics
MicroRNAs/*metabolism
Nociceptive Pain/*therapy
Protein Precursors/*genetics
Animals ; Antagomirs/administration & dosage ; Disease Models, Animal ; Down-Regulation ; Female ; Freund's Adjuvant/administration & dosage ; Freund's Adjuvant/immunology ; Humans ; Injections, Intraventricular ; MicroRNAs/agonists ; MicroRNAs/antagonists & inhibitors ; Nociception/drug effects ; Nociceptive Pain/diagnosis ; Nociceptive Pain/genetics ; Nociceptive Pain/immunology ; Pain Threshold/drug effects ; Rats
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Contributed Indexing:
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Keywords: Electroacupuncture tolerance; New target; Nociception; Proenkephalin; miRNA
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Substance Nomenclature:
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0 (Antagomirs)
0 (Enkephalins)
0 (MIRNLET7 microRNA, rat)
0 (MicroRNAs)
0 (Protein Precursors)
9007-81-2 (Freund's Adjuvant)
93443-35-7 (preproenkephalin)
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Entry Date(s):
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Date Created: 20200319 Date Completed: 20200601 Latest Revision: 20200601
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Update Code:
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20240105
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DOI:
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10.1016/j.gene.2020.144583
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PMID:
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32184167
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Background: Studies showed that increased let-7b-5p microRNA during repeated electroacupuncture (EA) treatment was associated the formation of EA tolerance, which manifested as gradually decreased nociceptive threshold. Proenkephalin (PENK) is the precursor of enkephalin which is a pivot neuropeptide responsible for the decreased nociceptive threshold in EA. The aim of this study was to evaluate the relationship between let-7b-5p and PENK in EA tolerance.
Methods: The target gene of let-7b-5p microRNA was determined through the dual-luciferase reporter assay in cortical neurons. Seventy-two Sprague Dawley rats received a combination of EA and intracerebroventricular injection of microRNA (let-7b-5p agomir, antagomir or their controls). The nociceptive thresholds were assessed with radiant heat tail-flick method. PENK and let-7b-5p were measured with Western Blot and qPCR, respectively, after administration of let-7b-5p agomir, antagomir, and their controls at day 1, 4 and 7.
Results: Let-7b-5p targeted the 3' untranslated region of Penk1. The nociceptive thresholds in Let-7b-5p agomir + EA group were decreased (p < 0.05) compared with those in Let-7b-5p antagomir + EA group at day 1 to 7. Compared with Let-7b-5p agomir + EA group, the expression level of PENK in Let-7b-5p antagomir + EA group was increased at days 1, 4, and 7 (p < 0.05) CONCLUSION: Let-7b-5p may be a new potential target for decreasing the EA tolerance effect and facilitating the application of EA in treating chronic nociception of patients.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
