-
Tytuł:
-
WHIMP links the actin nucleation machinery to Src-family kinase signaling during protrusion and motility.
-
Autorzy:
-
Kabrawala S; Department of Molecular and Cell Biology, Institute for Systems Genomics, University of Connecticut, Storrs, Connecticut, United States of America.
Zimmer MD; Department of Molecular and Cell Biology, Institute for Systems Genomics, University of Connecticut, Storrs, Connecticut, United States of America.
Campellone KG; Department of Molecular and Cell Biology, Institute for Systems Genomics, University of Connecticut, Storrs, Connecticut, United States of America.
-
Źródło:
-
PLoS genetics [PLoS Genet] 2020 Mar 20; Vol. 16 (3), pp. e1008694. Date of Electronic Publication: 2020 Mar 20 (Print Publication: 2020).
-
Typ publikacji:
-
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
-
Język:
-
English
-
Imprint Name(s):
-
Original Publication: San Francisco, CA : Public Library of Science, c2005-
-
MeSH Terms:
-
Actin Cytoskeleton/*metabolism
Cell Movement/*physiology
Cell Surface Extensions/*physiology
Wiskott-Aldrich Syndrome Protein/*metabolism
src-Family Kinases/*metabolism
Actin-Related Protein 2-3 Complex/metabolism ; Actins/metabolism ; Animals ; Cell Line ; Endocytosis/physiology ; Male ; Mice ; Mice, Inbred C57BL ; NIH 3T3 Cells ; Protein Domains ; Signal Transduction ; Wiskott-Aldrich Syndrome Protein Family/metabolism
-
References:
-
J Biol Chem. 2002 Nov 22;277(47):45115-21. (PMID: 12235133)
Nature. 2000 Mar 9;404(6774):151-8. (PMID: 10724160)
Cell Motil Cytoskeleton. 2006 Jan;63(1):6-13. (PMID: 16317717)
Nat Rev Mol Cell Biol. 2014 Sep;15(9):577-90. (PMID: 25145849)
Curr Opin Cell Biol. 2019 Feb;56:53-63. (PMID: 30278304)
Dev Cell. 2009 Nov;17(5):712-23. (PMID: 19922875)
EMBO J. 2003 Jul 15;22(14):3602-12. (PMID: 12853475)
Curr Biol. 2013 Oct 21;23(20):1990-8. (PMID: 24120641)
Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3834-43. (PMID: 27325766)
Proc Natl Acad Sci U S A. 2005 Jan 25;102(4):1098-103. (PMID: 15657136)
Nature. 2010 Nov 25;468(7323):533-8. (PMID: 21107423)
Cell. 2008 Jul 11;134(1):148-61. (PMID: 18614018)
Blood. 1997 Jul 1;90(1):70-5. (PMID: 9207440)
Proc Natl Acad Sci U S A. 1998 May 26;95(11):6181-6. (PMID: 9600938)
Nat Cell Biol. 2009 Apr;11(4):451-9. (PMID: 19287377)
J Med Chem. 2006 Nov 2;49(22):6465-88. (PMID: 17064066)
Proc Natl Acad Sci U S A. 2004 Mar 30;101(13):4379-83. (PMID: 15070726)
J Cell Sci. 2009 Apr 15;122(Pt 8):1059-69. (PMID: 19339545)
J Biol Chem. 2006 Oct 20;281(42):31290-7. (PMID: 16899465)
Nature. 1998 Jan 1;391(6662):93-6. (PMID: 9422512)
Sci Transl Med. 2010 Jun 23;2(37):37ra44. (PMID: 20574068)
Mol Biol Cell. 2011 Dec;22(23):4563-74. (PMID: 21965285)
Nat Cell Biol. 2001 Jan;3(1):76-82. (PMID: 11146629)
Mol Biol Cell. 2009 Jul;20(14):3209-23. (PMID: 19458196)
Cell Adh Migr. 2018;12(5):401-416. (PMID: 29513145)
Nat Struct Biol. 2003 Aug;10(8):591-8. (PMID: 12872157)
Cancer Res. 2002 Feb 1;62(3):669-74. (PMID: 11830518)
Dev Cell. 2003 Oct;5(4):595-609. (PMID: 14536061)
Mol Biol Cell. 2017 Sep 15;28(19):2492-2507. (PMID: 28720660)
Dev Cell. 2009 Nov;17(5):699-711. (PMID: 19922874)
Genes Cells. 2005 May;10(5):381-92. (PMID: 15836768)
Nat Commun. 2014;5:3308. (PMID: 24518936)
J Biol Chem. 1999 Nov 12;274(46):32531-4. (PMID: 10551802)
Cell. 2014 Jan 16;156(1-2):195-207. (PMID: 24439376)
Mol Cell Biol. 2005 Oct;25(20):8834-43. (PMID: 16199863)
Nature. 2002 Aug 15;418(6899):790-3. (PMID: 12181570)
Proc Natl Acad Sci U S A. 2011 Aug 30;108(35):14449-54. (PMID: 21844371)
Nat Rev Mol Cell Biol. 2013 Jan;14(1):7-12. (PMID: 23212475)
Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):3739-44. (PMID: 10097107)
J Neurosci. 2003 Apr 15;23(8):3343-52. (PMID: 12716942)
Cell. 1999 Apr 16;97(2):221-31. (PMID: 10219243)
Mol Biol Cell. 2010 Aug 15;21(16):2880-93. (PMID: 20573979)
J Biol Chem. 2006 Feb 10;281(6):3513-20. (PMID: 16293614)
Trends Cell Biol. 2018 Feb;28(2):79-98. (PMID: 29162307)
J Cell Sci. 2011 Nov 15;124(Pt 22):3753-9. (PMID: 22114305)
Mol Cell. 2004 Oct 22;16(2):269-79. (PMID: 15494313)
Nat Cell Biol. 2005 Oct;7(10):969-76. (PMID: 16155590)
Annu Rev Cell Dev Biol. 2010;26:315-33. (PMID: 19575647)
PLoS Pathog. 2018 Dec 14;14(12):e1007485. (PMID: 30550556)
Mol Biol Cell. 2012 Aug;23(16):3215-28. (PMID: 22718907)
BMC Res Notes. 2012 Feb 08;5:88. (PMID: 22316129)
Curr Biol. 1998 Dec 17-31;8(25):1347-56. (PMID: 9889097)
Methods Mol Biol. 2013;1046:273-93. (PMID: 23868594)
EMBO J. 2004 Feb 25;23(4):749-59. (PMID: 14765121)
EMBO J. 1998 Dec 1;17(23):6932-41. (PMID: 9843499)
J Cell Biol. 2000 Sep 18;150(6):1299-310. (PMID: 10995436)
J Biol Chem. 1996 Jan 12;271(2):695-701. (PMID: 8557675)
EMBO J. 1996 Oct 1;15(19):5326-35. (PMID: 8895577)
J Cell Biol. 2012 Oct 1;199(1):111-24. (PMID: 23027905)
Dev Cell. 2002 Nov;3(5):645-58. (PMID: 12431372)
J Cell Sci. 2007 Feb 15;120(Pt 4):658-69. (PMID: 17264147)
Curr Biol. 2004 Apr 20;14(8):697-703. (PMID: 15084285)
Genes Cells. 2009 Oct;14(10):1197-207. (PMID: 19778379)
Nat Cell Biol. 2004 May;6(5):420-6. (PMID: 15107862)
Brain. 2015 Aug;138(Pt 8):2173-90. (PMID: 26070982)
Biochemistry. 2010 Mar 16;49(10):2227-34. (PMID: 20146487)
Curr Biol. 2015 Jun 29;25(13):1791-7. (PMID: 26096974)
J Cell Biol. 2005 Jan 31;168(3):441-52. (PMID: 15684033)
Mol Biol Cell. 2012 Dec;23(24):4807-19. (PMID: 23087206)
Science. 2009 Nov 27;326(5957):1208-12. (PMID: 19965462)
EMBO J. 2004 Feb 25;23(4):760-71. (PMID: 14765113)
J Cell Sci. 2017 Jul 15;130(14):2235-2241. (PMID: 28646090)
Dev Cell. 2019 Nov 18;51(4):431-445.e7. (PMID: 31668663)
Cytoskeleton (Hoboken). 2010 Mar;67(3):193-206. (PMID: 20175130)
Oncogene. 2011 Dec 1;30(48):4835-42. (PMID: 21625218)
Cell. 1994 Aug 26;78(4):635-44. (PMID: 8069912)
Trends Biochem Sci. 2016 Jun;41(6):478-490. (PMID: 27068179)
Nat Cell Biol. 2001 Oct;3(10):897-904. (PMID: 11584271)
Sci STKE. 2005 Jan 11;2005(266):pl1. (PMID: 15644491)
Mol Biol Cell. 2016 Mar 15;27(6):967-78. (PMID: 26823012)
J Cell Biol. 2008 Mar 24;180(6):1245-60. (PMID: 18362183)
Cytoskeleton (Hoboken). 2012 Dec;69(12):1047-58. (PMID: 23012235)
Eur J Cell Biol. 2014 May-Jun;93(5-6):194-204. (PMID: 25015719)
Biochem Biophys Res Commun. 1999 Jun 24;260(1):296-302. (PMID: 10381382)
J Cell Biol. 2019 Jan 7;218(1):251-266. (PMID: 30420355)
PLoS Genet. 2007 Dec;3(12):e237. (PMID: 18159949)
J Cell Biol. 2018 Feb 5;217(2):447-457. (PMID: 29233866)
Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1731-6. (PMID: 15659545)
Nat Commun. 2015 Jul 30;6:7888. (PMID: 26223951)
Curr Biol. 2018 Nov 19;28(22):3674-3684.e6. (PMID: 30393033)
Mol Cell. 2009 Nov 13;36(3):512-24. (PMID: 19917258)
Blood. 2014 Nov 27;124(23):3409-19. (PMID: 25253772)
Biochemistry. 2007 Mar 20;46(11):3494-502. (PMID: 17302440)
Nat Rev Immunol. 2010 Mar;10(3):182-92. (PMID: 20182458)
Curr Biol. 2013 Jan 21;23(2):107-17. (PMID: 23273897)
Small GTPases. 2020 May;11(3):160-166. (PMID: 29172947)
Nature. 2003 Jul 24;424(6947):452-6. (PMID: 12879075)
Nat Rev Mol Cell Biol. 2010 Apr;11(4):237-51. (PMID: 20237478)
Elife. 2013 Sep 03;2:e00884. (PMID: 24015358)
Curr Biol. 2007 Apr 3;17(7):579-91. (PMID: 17398099)
Science. 2000 Oct 27;290(5492):801-6. (PMID: 11052943)
J Cell Biol. 2017 Jun 5;216(6):1673-1688. (PMID: 28473602)
Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10442-7. (PMID: 20498093)
Curr Opin Cell Biol. 2014 Oct;30:74-82. (PMID: 24999834)
-
Grant Information:
-
K02 AG050774 United States AG NIA NIH HHS; R01 GM107441 United States GM NIGMS NIH HHS
-
Substance Nomenclature:
-
0 (Actin-Related Protein 2-3 Complex)
0 (Actins)
0 (Was protein, mouse)
0 (Wasf1 protein, mouse)
0 (Wiskott-Aldrich Syndrome Protein)
0 (Wiskott-Aldrich Syndrome Protein Family)
EC 2.7.10.2 (src-Family Kinases)
-
Entry Date(s):
-
Date Created: 20200321 Date Completed: 20200721 Latest Revision: 20200828
-
Update Code:
-
20240105
-
PubMed Central ID:
-
PMC7112243
-
DOI:
-
10.1371/journal.pgen.1008694
-
PMID:
-
32196488
-
Cell motility is governed by cooperation between the Arp2/3 complex and nucleation-promoting factors from the Wiskott-Aldrich Syndrome Protein (WASP) family, which together assemble actin filament networks to drive membrane protrusion. Here we identify WHIMP (WAVE Homology In Membrane Protrusions) as a new member of the WASP family. The Whimp gene is encoded on the X chromosome of a subset of mammals, including mice. Murine WHIMP promotes Arp2/3-dependent actin assembly, but is less potent than other nucleation factors. Nevertheless, WHIMP-mediated Arp2/3 activation enhances both plasma membrane ruffling and wound healing migration, whereas WHIMP depletion impairs protrusion and slows motility. WHIMP expression also increases Src-family kinase activity, and WHIMP-induced ruffles contain the additional nucleation-promoting factors WAVE1, WAVE2, and N-WASP, but not JMY or WASH. Perturbing the function of Src-family kinases, WAVE proteins, or Arp2/3 complex inhibits WHIMP-driven ruffling. These results suggest that WHIMP-associated actin assembly plays a direct role in membrane protrusion, but also results in feedback control of tyrosine kinase signaling to modulate the activation of multiple WASP-family members.
Competing Interests: The authors have declared that no competing interests exist.
Zaloguj się, aby uzyskać dostęp do pełnego tekstu.