-
Tytuł:
-
The Citrus Flavanone Hesperetin Induces Apoptosis in CTCL Cells via STAT3/Notch1/NFκB-Mediated Signaling Axis.
-
Autorzy:
-
Kottaiswamy A; VRR Institute of Biomedical Science, University of Madras, Chennai, India.
Kizhakeyil A; Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Clinical Sciences Building, 11 Mandalay Road, Nanyang Ave, Singapore.
Padmanaban AM; VRR Institute of Biomedical Science, University of Madras, Chennai, India.
Mirza FB; VRR Institute of Biomedical Science, University of Madras, Chennai, India.
Vijay VR; Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Clinical Sciences Building, 11 Mandalay Road, Nanyang Ave, Singapore.
Lee PS; Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Clinical Sciences Building, 11 Mandalay Road, Nanyang Ave, Singapore.
Verma NK; Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Clinical Sciences Building, 11 Mandalay Road, Nanyang Ave, Singapore.
Kalaiselvan P; Chettinad Hospital and Research Institute, Chennai, India.
Samuel S; VRR Institute of Biomedical Science, University of Madras, Chennai, India.
-
Źródło:
-
Anti-cancer agents in medicinal chemistry [Anticancer Agents Med Chem] 2020; Vol. 20 (12), pp. 1459-1468.
-
Typ publikacji:
-
Journal Article; Research Support, Non-U.S. Gov't
-
Język:
-
English
-
Imprint Name(s):
-
Publication: Amsterdam : Bentham Science Publishers
Original Publication: Sharjah, U.A.E. ; San Francisco, CA : Bentham Science Publishers, [2006]-
-
MeSH Terms:
-
Antineoplastic Agents, Phytogenic/*pharmacology
Apoptosis/*drug effects
Citrus/*chemistry
Hesperidin/*pharmacology
NF-kappa B/*antagonists & inhibitors
Receptor, Notch1/*antagonists & inhibitors
STAT3 Transcription Factor/*antagonists & inhibitors
Antineoplastic Agents, Phytogenic/chemistry ; Cell Cycle Checkpoints/drug effects ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Dose-Response Relationship, Drug ; Drug Screening Assays, Antitumor ; Hesperidin/chemistry ; Humans ; Molecular Structure ; NF-kappa B/metabolism ; Receptor, Notch1/metabolism ; STAT3 Transcription Factor/metabolism ; Signal Transduction/drug effects ; Structure-Activity Relationship ; Tumor Cells, Cultured
-
Contributed Indexing:
-
Keywords: Apoptosis; CTCL; NFκB; Notch1; ROS; STAT3
-
Substance Nomenclature:
-
0 (Antineoplastic Agents, Phytogenic)
0 (NF-kappa B)
0 (NOTCH1 protein, human)
0 (Receptor, Notch1)
0 (STAT3 Transcription Factor)
0 (STAT3 protein, human)
E750O06Y6O (Hesperidin)
Q9Q3D557F1 (hesperetin)
-
Entry Date(s):
-
Date Created: 20200326 Date Completed: 20210601 Latest Revision: 20221222
-
Update Code:
-
20240105
-
DOI:
-
10.2174/1871521409666200324110031
-
PMID:
-
32208126
-
Background: Hesperetin is a natural compound known for its cholesterol-lowering effect and a wide range of pharmacological activities.
Objectives: Investigating the potential anticancer activities of Hesperetin in malignant hematolymphoid cell lines HuT78 and MJ, derived from patients with Cutaneous T-Cell Lymphomas (CTCL).
Methods: The cytotoxic effect of Hesperetin on two different CTCL cell lines, HuT78 and MJ, was assessed by MTS-based colorimetric assay. Apoptosis, cell cycle, ROS (Reactive Oxygen Species) and molecular analysis were performed using flow-cytometry and immunoblotting.
Results: Hesperetin-treated CTCL cells were arrested at the sub-G1 phase of cell cycle with the concomitant decrease in the expression of the cell cycle regulator protein cyclin B. In addition, the study found that the cellular treatment with Hesperetin caused an induction of apoptosis, which was independent of ROS generation. Hesperetin caused a significant decrease in the expression level of anti-apoptotic protein Bcl-xL and an increase in cleaved caspase-3 and PARP proteins in CTCL cells. Furthermore, Hesperetin treatment in CTCL cells down-regulated the expression of Notch1 and phosphorylation of STAT3 (Tyr705) and inhibited NFκBp65.
Conclusion: This study highlights the anticancer properties of Hesperetin. Which induces apoptosis in CTCL cells via STAT3/Notch1/NFκB mediated signaling pathway, suggesting that further development of this novel class of flavonoid may contribute to new drug discovery for certain hematolymphoid malignancies.
(Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
