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Tytuł:
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Differential responses of gut microbiota of male and female fathead minnow (Pimephales promelas) to a short-term environmentally-relevant, aqueous exposure to benzo[a]pyrene.
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Autorzy:
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DeBofsky A; Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Xie Y; Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. Electronic address: .
Grimard C; Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Alcaraz AJ; Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Brinkmann M; Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada; School of Environment and Sustainability, University of Saskatchewan, Saskatoon, Saskatchewan, Canada; Global Institute for Water Security, University of Saskatchewan, Saskatoon, Canada.
Hecker M; Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada; School of Environment and Sustainability, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Giesy JP; Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada; Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada; Department of Environmental Sciences, Baylor University, Waco, TX, USA.
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Źródło:
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Chemosphere [Chemosphere] 2020 Aug; Vol. 252, pp. 126461. Date of Electronic Publication: 2020 Mar 12.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Oxford : Elsevier Science Ltd
Original Publication: Oxford, New York, : Pergamon Press.
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MeSH Terms:
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Benzo(a)pyrene/*toxicity
Cyprinidae/*physiology
Gastrointestinal Microbiome/*drug effects
Water Pollutants, Chemical/*toxicity
Animals ; Cyprinidae/microbiology ; Cytochrome P-450 CYP1A1/metabolism ; Environmental Exposure ; Female ; Male ; Polycyclic Aromatic Hydrocarbons ; RNA, Ribosomal, 16S ; Water
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Contributed Indexing:
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Keywords: Freshwater fish; Homeostasis; Molecular ecology; Next generation sequencing; Persistence organics pollution
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Substance Nomenclature:
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0 (Polycyclic Aromatic Hydrocarbons)
0 (RNA, Ribosomal, 16S)
0 (Water Pollutants, Chemical)
059QF0KO0R (Water)
3417WMA06D (Benzo(a)pyrene)
EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
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Entry Date(s):
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Date Created: 20200328 Date Completed: 20200610 Latest Revision: 20200610
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Update Code:
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20240105
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DOI:
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10.1016/j.chemosphere.2020.126461
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PMID:
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32213373
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In addition to aiding in digestion of food and uptake of nutrients, microbiota in guts of vertebrates are responsible for regulating several beneficial functions, including development of an organism and maintaining homeostasis. However, little is known about effects of exposures to chemicals on structure and function of gut microbiota of fishes. To assess effects of exposure to polycyclic aromatic hydrocarbons (PAHs) on gut microbiota, male and female fathead minnows (Pimephales promelas) were exposed to environmentally-relevant concentrations of the legacy PAH benzo[a]pyrene (BaP) in water. Measured concentrations of BaP ranged from 2.3 × 10 -3 to 1.3 μg L -1 . The community of microbiota in the gut were assessed by use of 16S rRNA metagenetics. Exposure to environmentally-relevant aqueous concentrations of BaP did not alter expression levels of mRNA for cyp1a1, a "classic" biomarker of exposure to BaP, but resulted in shifts in relative compositions of gut microbiota in females rather than males. Results presented here illustrate that in addition to effects on more well-studied molecular endpoints, relative compositions of the microbiota in guts of fish can also quickly respond to exposure to chemicals, which can provide additional mechanisms for adverse effects on individuals.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
