Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Przeglądasz jako GOŚĆ
Tytuł pozycji:

Propionate enhances the expression of key genes involved in the gluconeogenic pathway in bovine intestinal epithelial cells.

Tytuł :
Propionate enhances the expression of key genes involved in the gluconeogenic pathway in bovine intestinal epithelial cells.
Autorzy :
Zhan K; Institute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Yang TY; Institute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Chen Y; Institute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Jiang MC; Institute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Zhao GQ; Institute of Animal Culture Collection and Application, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. Electronic address: .
Pokaż więcej
Źródło :
Journal of dairy science [J Dairy Sci] 2020 Jun; Vol. 103 (6), pp. 5514-5524. Date of Electronic Publication: 2020 Apr 08.
Typ publikacji :
Journal Article
Język :
English
Imprint Name(s) :
Publication: Champaign, IL : American Dairy Science Association
Original Publication: Lancaster, Pa. [etc.]
MeSH Terms :
Cattle/*physiology
Fatty Acids, Volatile/*metabolism
Gene Expression Regulation, Enzymologic/*drug effects
Gluconeogenesis/*drug effects
Propionates/*pharmacology
Animals ; Cattle/genetics ; Cells, Cultured ; Epithelial Cells/drug effects ; Epithelial Cells/enzymology ; Female ; Gluconeogenesis/genetics ; Intestines/drug effects ; Intestines/enzymology ; Monocarboxylic Acid Transporters/genetics ; Phosphoenolpyruvate Carboxykinase (ATP)/genetics ; Pyruvate Carboxylase/genetics ; RNA, Messenger/genetics ; Sodium-Hydrogen Exchanger 1/genetics
Contributed Indexing :
Keywords: bovine intestinal epithelial cell; gluconeogenesis; immortal; propionate
Substance Nomenclature :
0 (Fatty Acids, Volatile)
0 (Monocarboxylic Acid Transporters)
0 (Propionates)
0 (RNA, Messenger)
0 (Sodium-Hydrogen Exchanger 1)
EC 4.1.1.49 (Phosphoenolpyruvate Carboxykinase (ATP))
EC 6.4.1.1 (Pyruvate Carboxylase)
Entry Date(s) :
Date Created: 20200413 Date Completed: 20200929 Latest Revision: 20200929
Update Code :
20210210
DOI :
10.3168/jds.2019-17309
PMID :
32278554
Czasopismo naukowe
Approximately 15 to 50% of short-chain fatty acids (SCFA) reach the ruminant small intestine. Previous research suggests that activation of small intestinal gluconeogenesis induced by propionate has beneficial effects on energy homeostasis. However, the regulatory effect of propionate on key gluconeogenic genes in enterocytes of the bovine small intestine remains less known. Therefore, the purpose of this study was to establish the long-term cultures of bovine intestinal epithelial cells (BIEC) from bovine jejunum tissue using SV40T (1:200; Santa Cruz, Shanghai, China) and investigate the regulatory effect of propionate on the key gluconeogenic genes in BIEC. Our study showed that long-term BIEC cultures were established by SV40T-induced immortalization. Immortal BIEC were distinguished by the expression of cytokeratin 18, villin, fatty acid binding protein 2, and small intestine peptidase. The mRNA expression of genes involved in the SCFA transporters, monocarboxylate transporter 4, and Na + /H + exchanger isoforms 1 were significantly elevated with 20 mM SCFA compared with untreated controls. In addition, BIEC exhibited significant uptake of propionate and butyrate from the culture medium. Remarkably, 3 mM propionate induced profound changes in mRNA level of key genes involved in gluconeogenesis, including phosphoenolpyruvate carboxykinase 2, pyruvate carboxylase, fructose-1,6-bisphosphatase 1, and peroxisome proliferator-activated receptor-γ coactivator 1α. Additionally, 3 mM propionate enhanced the expression of PGC1A mRNA at 3, 6, 12, and 24 h of incubation. These findings suggest that propionate controls the mRNA expression of genes involved in key enzymes for gluconeogenesis in the enterocytes of bovines.
(Copyright © 2020 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies