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Tytuł:
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Anti-inflammatory Effects of Extracts of Sweet Basil (Ocimum basilicum L.) on a Co-culture of 3T3-L1 Adipocytes and RAW264.7 Macrophages.
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Autorzy:
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Takeuchi H; Graduate school of Kanagawa Institute of Technology.
Takahashi-Muto C; Department of Clinical Nutrition, Kitasato Junior College of Health and Hygicnic Sciences.
Nagase M; Department of Nutrition and Life Science, Kanagawa Institute of Technology.
Kassai M; S&B food Inc.
Tanaka-Yachi R; Department of Applied Biochemistry, Kanagawa Institute of Technology.
Kiyose C; Graduate school of Kanagawa Institute of Technology.; Department of Nutrition and Life Science, Kanagawa Institute of Technology.
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Źródło:
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Journal of oleo science [J Oleo Sci] 2020 May 02; Vol. 69 (5), pp. 487-493. Date of Electronic Publication: 2020 Apr 13.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Tokyo : Japan Oil Chemists' Society
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MeSH Terms:
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Anti-Inflammatory Agents*
Coculture Techniques*
Adipocytes/*metabolism
Cytokines/*metabolism
Inflammation Mediators/*metabolism
Ocimum/*chemistry
Plant Extracts/*pharmacology
3T3 Cells ; Animals ; Cytokines/genetics ; Gene Expression/drug effects ; Mice ; NF-kappa B/genetics ; NF-kappa B/metabolism ; RAW 264.7 Cells ; RNA, Messenger/metabolism ; Tumor Necrosis Factor Receptor Superfamily, Member 9/genetics ; Tumor Necrosis Factor Receptor Superfamily, Member 9/metabolism
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Contributed Indexing:
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Keywords: adipocytes; inflammation; macrophages; obesity; sweet basil
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Substance Nomenclature:
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0 (Anti-Inflammatory Agents)
0 (Cytokines)
0 (Inflammation Mediators)
0 (NF-kappa B)
0 (Plant Extracts)
0 (RNA, Messenger)
0 (Tnfrsf9 protein, mouse)
0 (Tumor Necrosis Factor Receptor Superfamily, Member 9)
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Entry Date(s):
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Date Created: 20200414 Date Completed: 20200914 Latest Revision: 20200914
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Update Code:
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20240105
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DOI:
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10.5650/jos.ess19321
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PMID:
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32281564
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Obesity, a lifestyle disease resulting from excessive caloric intake and insufficient physical activity, results in a state of chronic inflammation. A food ingredient that suppresses chronic inflammation could help prevent associated diseases. Sweet basil (Ocimum basilicum L.) is a herb from the Lamiaceae family with some reported anti-inflammatory effects. Via this in vitro study, we aimed to investigate whether sweet basil exerts anti-inflammatory effects in obese patients. Fresh sweet basil leaves were freeze-dried and powered. After that, this was extracted with 80% methanol. After 3T3-L1 adipocytes were cultured with sweet basil extracts at final concentrations of either 5 or 25 μg/mL for 24h, RAW264.7 macrophages were seeded onto this adipocytes and co-cultured for 12h. We determined the effects of sweet basil extracts on inflammatory cytokine expression by real-time PCR or western blotting. Sweet basil extracts reduced the expression of inflammatory cytokine mRNA induced by co-culture, including that of IL-6 (Il6), IL-1β (Il1b), TNF-α (Tnf), and CCL2 (Ccl2). In addition, sweet basil extracts suppressed the mRNA expression of NF-κB (Nfκb1), a transcription factor of inflammatory cytokines. In an investigation of costimulatory CD137 (Tnfrsf9)/CD137L inflammatory signaling, a member of the TNF super-family, sweet basil extracts inhibited Tnfrsf9 expression induced by the co-culture. Therefore, the results of this study indicated that sweet basil extracts have an anti-inflammatory effect against adipocyte-induced inflammation, possibly through suppression of Tnfrsf9 expression.