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Tytuł:
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MicroRNA-212 targets SIRT2 to influence lipogenesis in bovine mammary epithelial cell line.
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Autorzy:
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Lu X; College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu225002, China.
Xia H; College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu225002, China.
Jiang J; College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu225002, China.
Xu X; College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu225002, China.
Li M; College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu225002, China.
Chen Z; College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu225002, China.
Sun Y; Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou University, Yangzhou225009, Jiangsu Province, China.
Zhang H; College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu225002, China.
Yang Z; College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu225002, China.
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Źródło:
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The Journal of dairy research [J Dairy Res] 2020 May; Vol. 87 (2), pp. 232-238. Date of Electronic Publication: 2020 Apr 16.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: London : Cambridge University Press
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MeSH Terms:
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Cattle/*genetics
Lipogenesis/*genetics
Mammary Glands, Animal/*metabolism
MicroRNAs/*physiology
Sirtuin 2/*genetics
Animals ; Cell Line ; Epithelial Cells/metabolism ; Fatty Acid Synthases/genetics ; Female ; Gene Expression Regulation/genetics ; MicroRNAs/genetics ; RNA, Small Interfering/genetics ; Sirtuin 2/physiology ; Sterol Regulatory Element Binding Protein 1/genetics ; Transfection
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Contributed Indexing:
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Keywords: Lipogenesis; SIRT2; miR-212; miR-375; miR-655
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Substance Nomenclature:
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0 (MicroRNAs)
0 (RNA, Small Interfering)
0 (Sterol Regulatory Element Binding Protein 1)
EC 2.3.1.85 (Fatty Acid Synthases)
EC 3.5.1.- (Sirtuin 2)
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Entry Date(s):
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Date Created: 20200417 Date Completed: 20210311 Latest Revision: 20210311
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Update Code:
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20240104
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DOI:
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10.1017/S0022029920000229
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PMID:
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32295660
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In this research paper we filter and verify miRNAs which may target silent information regulator homolog 2 (SIRT2) gene and then describe the mechanism whereby miRNA-212 might regulate lipogenic genes in mammary epithelial cell lines via targeting SIRT2. Bioinformatics analysis revealed that the bovine SIRT2 gene is regulated by three miRNAs: miR-212, miR-375 and miR-655. The three miRNAs were verified and screened by qRT-PCR, western blot, and luciferase multiplex verification techniques and only miR-212 was shown to have a targeting relationship with SIRT2. The results of co-transfecting miR-212 and silencing RNA (siRNA) showed that by targeting SIRT2, miR-212 can regulate the expression of fatty acid synthetase (FASN) and sterol regulatory element binding factor 1 (SREBP1) but not peroxisome proliferator-activated receptor gamma (PPARγ). Measurement of triglyceride (TAG) content showed that miR-212 increased the fat content of mammary epithelial cell lines. The study indicates that miR-212 could target and inhibit the expression of the SIRT2 gene to promote lipogenesis in mammary epithelial cell lines.
