Effect of Ancylostoma ceylanicum hookworm platelet inhibitor on platelet adhesion and peripheral blood mononuclear cell proliferation.

Szczegóły
Abstrakt

Tytuł:: Effect of Ancylostoma ceylanicum hookworm platelet inhibitor on platelet adhesion and peripheral blood mononuclear cell proliferation.
Autorzy:: Huang Y; Guangdong Provincial Zoonosis Prevention and Control Key Laboratory, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510542, China.
Abuzeid AMI; Guangdong Provincial Zoonosis Prevention and Control Key Laboratory, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510542, China.
Zhuang T; Guangdong Provincial Zoonosis Prevention and Control Key Laboratory, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510542, China.
Zhu S; Guangdong Provincial Zoonosis Prevention and Control Key Laboratory, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510542, China.
He L; Guangdong Provincial Zoonosis Prevention and Control Key Laboratory, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510542, China.
Liu Y; Guangdong Provincial Zoonosis Prevention and Control Key Laboratory, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510542, China.
Zhao Q; Guangdong Provincial Zoonosis Prevention and Control Key Laboratory, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510542, China.
Chen X; Guangdong Provincial Zoonosis Prevention and Control Key Laboratory, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510542, China.
Li G; Guangdong Provincial Zoonosis Prevention and Control Key Laboratory, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510542, China. .
Źródło:: Parasitology research [Parasitol Res] 2020 Jun; Vol. 119 (6), pp. 1777-1784. Date of Electronic Publication: 2020 Apr 16.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Berlin : Springer International, c1987-
MeSH Terms:: Ancylostoma/*chemistry
Cell Proliferation/*drug effects
Helminth Proteins/*pharmacology
Platelet Adhesiveness/*drug effects
Platelet Aggregation Inhibitors/*pharmacology
Ancylostoma/genetics ; Animals ; Cytokines/metabolism ; Dogs ; Helminth Proteins/genetics ; Helminth Proteins/metabolism ; Leukocytes, Mononuclear/cytology ; Leukocytes, Mononuclear/drug effects ; Leukocytes, Mononuclear/metabolism ; Platelet Aggregation Inhibitors/isolation & purification ; Recombinant Proteins/genetics ; Recombinant Proteins/isolation & purification ; Recombinant Proteins/metabolism ; Recombinant Proteins/pharmacology
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Grant Information:: 31672541 National Natural Science Foundation of China
Contributed Indexing:: Keywords: Ancylostoma ceylanicum; Cytokine; Hookworm platelet inhibitor; PBMC; Platelet adhesion
Substance Nomenclature:: 0 (Cytokines)
0 (Helminth Proteins)
0 (Platelet Aggregation Inhibitors)
0 (Recombinant Proteins)
Entry Date(s):: Date Created: 20200418 Date Completed: 20200731 Latest Revision: 20220124
Update Code:: 20240104
DOI:: 10.1007/s00436-020-06678-4
PMID:: 32300877
: Czasopismo naukowe

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Ancylostoma ceylanicum is a zoonotic parasitic nematode that can cause iron-deficiency anemia and malnutrition in humans. A. ceylanicum hookworm platelet inhibitor (Ace-HPI) can inhibit platelet aggregation in the host to facilitate blood sucking, but whether it possesses platelet adhesion inhibitory activity or immunomodulatory role is yet unknown. To explore the effect of Ace-HPI on platelet adhesion, we expressed the recombinant protein in two competent cells, BL21 (DE3) and Rosetta-gami2 (DE3), and incubated this protein with canine platelets in a 96-well microplate. Ace-HPI was used to stimulate peripheral blood mononuclear cells (PBMC) in vitro to investigate the effect on PBMC proliferation and cytokine expression. Results showed that Ace-HPI expressed in Rosetta-gami2 (DE3) strain was mostly soluble. The inhibitory effect of this protein on platelet adhesion was relatively weak (7-8%). This protein stimulated the proliferation of PBMC and promoted the expression of Treg and Th2 cytokines, such as IL-10 and IL-13. These results lay a foundation for exploring the role of Ace-HPI in hookworm disease pathogenesis and as a candidate molecule for hookworm vaccines.

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