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Tytuł:
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Draft genome and description of Negativicoccus massiliensis strain Marseille-P2082, a new species isolated from the gut microbiota of an obese patient.
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Autorzy:
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Togo AH; IRD198, APHM, MEPHI, IHU-Méditerranée Infection, Aix Marseille Univ, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France.; IHU Méditerranée Infection, Marseille, France.
Diop A; IRD, SSA, VITROME, IHU-Méditerranée Infection, Aix Marseille Univ, Marseille, France.
Tall ML; IRD198, APHM, MEPHI, IHU-Méditerranée Infection, Aix Marseille Univ, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France.; IHU Méditerranée Infection, Marseille, France.
Million M; IRD198, APHM, MEPHI, IHU-Méditerranée Infection, Aix Marseille Univ, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France.; IHU Méditerranée Infection, Marseille, France.
Khelaifia S; IRD198, APHM, MEPHI, IHU-Méditerranée Infection, Aix Marseille Univ, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France.; IHU Méditerranée Infection, Marseille, France.
Maraninchi M; NORT 'Nutrition, Obesity and Risk of Thrombosis', INSERM1062, INRA1260, Aix Marseille Université, Marseille, France.
Raoult D; IRD198, APHM, MEPHI, IHU-Méditerranée Infection, Aix Marseille Univ, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France.; IHU Méditerranée Infection, Marseille, France.
Fournier PE; IHU Méditerranée Infection, Marseille, France.; IRD, SSA, VITROME, IHU-Méditerranée Infection, Aix Marseille Univ, Marseille, France.
Dubourg G; IRD198, APHM, MEPHI, IHU-Méditerranée Infection, Aix Marseille Univ, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France. .; IHU Méditerranée Infection, Marseille, France. .
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Źródło:
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Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2020 Jul; Vol. 113 (7), pp. 997-1008. Date of Electronic Publication: 2020 Apr 17.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: 2004- : Berlin : Springer
Original Publication: Wageningen, Netherland [etc.] Veenman [etc.]
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MeSH Terms:
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Gastrointestinal Microbiome*
Obesity*
Phylogeny*
Veillonellaceae/*classification
Veillonellaceae/*isolation & purification
Adult ; Bacterial Typing Techniques ; Base Composition ; DNA, Bacterial/genetics ; Feces/microbiology ; Female ; Genes, Bacterial/genetics ; Genome, Bacterial ; Genomics ; Humans ; RNA, Ribosomal, 16S/genetics ; Sequence Analysis, DNA ; Veillonellaceae/genetics ; Veillonellaceae/physiology
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Grant Information:
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IHU Méditerranée Infection 10-IAHU-03. « Investissements d'avenir »
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Contributed Indexing:
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Keywords: Bariatric surgery; Culturomics; Human gut microbiota; Negativicoccus massiliensis; Obesity; Taxonomy
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Substance Nomenclature:
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0 (DNA, Bacterial)
0 (RNA, Ribosomal, 16S)
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SCR Organism:
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Negativicoccus massiliensis
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Entry Date(s):
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Date Created: 20200419 Date Completed: 20210111 Latest Revision: 20210111
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Update Code:
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20240104
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DOI:
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10.1007/s10482-020-01414-5
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PMID:
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32303967
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Strain Marseille-P2082, an anaerobic, non-motile, asporogenous, Gram-negative, coccoid bacterium was isolated from the faeces of a 33 year-old obese French woman before bariatric surgery. The isolate exhibits 98.65% 16S rRNA gene nucleotide sequence similarity with Negativicoccus succinicivorans strain ADV 07/08/06-B-1388 T , its current closest phylogenetic neighbour with standing in nomenclature. However, the dDDH relatedness between the new isolate and N. succinicivorans type strain ADV 07/08/06-B-1388 T is 52.5 ± 2.7%. Strain Marseille-P2082 has a genome of 1,360,589 bp with a 51.1% G+C content. Its major fatty acids were identified as C 18:1n9 , C 18:0 and C 16:0 . Based on its phenotypic, genomic and phylogenetic characteristics, strain Marseille-P2082 T [= CSURP2082 (Collection de Souches de l'Unité des Rickettsies) = DSM 100853] is proposed as the type strain of the novel species Negativicoccus massiliensis sp. nov. The 16S rRNA gene sequence and whole-genome shotgun sequence have been deposited in EMBL-EBI under accession numbers LN876651 and LT700188, respectively.