Unbalanced human embryos secrete more hyperglycosylated human chorionic gonadotrophin (hCG-H) than balanced ones.

Szczegóły
Abstrakt

Tytuł:: Unbalanced human embryos secrete more hyperglycosylated human chorionic gonadotrophin (hCG-H) than balanced ones.
Autorzy:: Parvanov D; Nadezhda Women's Health Hospital, 3 'Blaga vest' Street, Sofia, Bulgaria. .
Nikolova D; Department of Medical Genetics, Medical Faculty, Medical University - Sofia, Sofia, Bulgaria.
Ganeva R; Nadezhda Women's Health Hospital, 3 'Blaga vest' Street, Sofia, Bulgaria.
Nikolova K; Nadezhda Women's Health Hospital, 3 'Blaga vest' Street, Sofia, Bulgaria.
Vasileva M; Nadezhda Women's Health Hospital, 3 'Blaga vest' Street, Sofia, Bulgaria.
Rangelov I; Nadezhda Women's Health Hospital, 3 'Blaga vest' Street, Sofia, Bulgaria.
Pancheva M; Nadezhda Women's Health Hospital, 3 'Blaga vest' Street, Sofia, Bulgaria.
Serafimova M; Nadezhda Women's Health Hospital, 3 'Blaga vest' Street, Sofia, Bulgaria.
Staneva R; Department of Medical Genetics, Medical Faculty, Medical University - Sofia, Sofia, Bulgaria.
Hadjidekova S; Department of Medical Genetics, Medical Faculty, Medical University - Sofia, Sofia, Bulgaria.
Scarpellini F; Centre for Endocrinology and Reproductive Medicine, Rome, Italy.
Stamenov G; Nadezhda Women's Health Hospital, 3 'Blaga vest' Street, Sofia, Bulgaria.
Źródło:: Journal of assisted reproduction and genetics [J Assist Reprod Genet] 2020 Jun; Vol. 37 (6), pp. 1341-1348. Date of Electronic Publication: 2020 Apr 22.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: <2004- >: Amsterdam : Springer
Original Publication: New York : Plenum Press, c1992-
MeSH Terms:: Embryo Culture Techniques*
Chorionic Gonadotropin/*genetics
Chorionic Gonadotropin, beta Subunit, Human/*genetics
Culture Media/*chemistry
Adult ; Biomarkers/metabolism ; Chorionic Gonadotropin/chemistry ; Chorionic Gonadotropin, beta Subunit, Human/chemistry ; Embryo Implantation/genetics ; Embryo Transfer ; Embryo, Mammalian ; Female ; Fertilization in Vitro ; Glycosylation ; High-Throughput Nucleotide Sequencing ; Humans ; Male ; Sperm Injections, Intracytoplasmic
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Contributed Indexing:: Keywords: Balanced embryos; Hyperglycosylated hCG; NGS; Non-invasive biomarkers; Unbalanced embryos
Substance Nomenclature:: 0 (Biomarkers)
0 (Chorionic Gonadotropin)
0 (Chorionic Gonadotropin, beta Subunit, Human)
0 (Culture Media)
Entry Date(s):: Date Created: 20200424 Date Completed: 20210203 Latest Revision: 20230727
Update Code:: 20240105
PubMed Central ID:: PMC7311563
DOI:: 10.1007/s10815-020-01776-9
PMID:: 32323120
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Purpose: The aim of this study was to compare the levels of hyperglycosylated human chorionic gonadotropin (hCG-H) secreted from balanced and unbalanced human embryos.
Methods: Single-step culture media samples from 155 good quality embryos, derived from 90 good prognosis patients undergoing intracytoplasmic sperm injection (ICSI), were collected on the fifth day of embryo cultivation. All embryos were tested by next-generation sequencing (NGS) technique. The hCG-H levels in the culture media were evaluated by ELISA kit (Cusabio Biotech, CBS-E15803h) according to the manufacturer's instructions. Statistical analysis was performed using SPSS v.21 (IBM Corp., Armonk, NY, USA).
Results: The NGS analysis revealed that 36% of the embryos (n = 56) were balanced, and 64% of the embryos were unbalanced (n = 99). The presence of hCG-H was confirmed in all embryo culture media samples but was absent in the negative control. In addition, hCG-H concentration was significantly higher in the culture media from unbalanced embryos compared with the balanced ones (0.72 ± 0.30 mIU/ml vs. 0.62 ± 0.12 mIU/ml, p = 0.02, respectively). Furthermore, the mean levels of hCG-H were significantly increased in the samples from embryos with multiple abnormalities. Finally, the highest levels of hCG-H were expressed from embryos with monosomy of chromosome 11 (1.28 ± 0.04 mIU/ml) and those with trisomies of chromosomes 21 (2.23 mIU/ml) and 4 (1.02 ± 0.35 mIU/ml).
Conclusion: Our results suggest that chromosomal aberrations in human embryos are associated with an increased secretion of hCG-H. However, hCG-H concentration in embryo culture media as a single biomarker is not sufficient for an accurate selection of balanced embryos.

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