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Tytuł pozycji:

Determination of amphenicol antibiotics and their glucuronide metabolites in urine samples using liquid chromatography with quadrupole time-of-flight mass spectrometry.

Tytuł:
Determination of amphenicol antibiotics and their glucuronide metabolites in urine samples using liquid chromatography with quadrupole time-of-flight mass spectrometry.
Autorzy:
Pastor-Belda M; Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, E-30100 Murcia, Spain.
Campillo N; Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, E-30100 Murcia, Spain.
Arroyo-Manzanares N; Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, E-30100 Murcia, Spain.
Hernández-Córdoba M; Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, E-30100 Murcia, Spain.
Viñas P; Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, E-30100 Murcia, Spain. Electronic address: .
Źródło:
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2020 Jun 01; Vol. 1146, pp. 122122. Date of Electronic Publication: 2020 Apr 18.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Original Publication: Amsterdam ; New York : Elsevier, c2002-
MeSH Terms:
Anti-Bacterial Agents/*urine
Chloramphenicol/*urine
Glucuronides/*urine
Tandem Mass Spectrometry/*methods
Chromatography, High Pressure Liquid ; Glucuronidase/metabolism ; Humans ; Hydrolysis ; Limit of Detection ; Liquid Phase Microextraction ; Metabolomics ; Methanol/chemistry ; Methyl n-Butyl Ketone/chemistry ; Reference Standards ; Solvents/chemistry ; Sulfatases/metabolism ; Thiamphenicol/analogs & derivatives ; Thiamphenicol/urine
Contributed Indexing:
Keywords: Amphenicols; Dispersive liquid-liquid microextraction; Glucuronide; Human urine; Liquid chromatography; Quadrupole time-of-flight mass spectrometry
Substance Nomenclature:
0 (Anti-Bacterial Agents)
0 (Glucuronides)
0 (Solvents)
66974FR9Q1 (Chloramphenicol)
6QDY60NH6N (Methyl n-Butyl Ketone)
9J97307Y1H (florfenicol)
EC 3.1.6.- (Sulfatases)
EC 3.2.1.31 (Glucuronidase)
FLQ7571NPM (Thiamphenicol)
U5T7B88CNP (methyl isobutyl ketone)
Y4S76JWI15 (Methanol)
Entry Date(s):
Date Created: 20200426 Date Completed: 20200723 Latest Revision: 20200723
Update Code:
20240105
DOI:
10.1016/j.jchromb.2020.122122
PMID:
32334391
Czasopismo naukowe
A rapid procedure for the determination of amphenicol antibiotics in human urine by liquid chromatography with quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) is proposed. The presence of thiamphenicol (TAP), florfenicol (FF) and chloramphenicol (CAP) in the human body can be attributed to their administration to treat certain diseases or by eating food of animal origin. The TAP, FF and CAP excreted in urine is mainly in the form of glucuronide conjugates, although their free forms may also be excreted to a lesser extent. In the procedure described, the enzymatic hydrolysis of amphenicol glucuronide forms in urine was carried out using β-glucuronidase and sulfatase at pH 5 (37 °C, overnight) in order to discriminate the free and conjugated forms. Then, amphenicol antibiotics were submitted to dispersive liquid-liquid microextraction (DLLME) for preconcentration. All the parameters affecting DLLME efficiency were optimized, and the following conditions were selected: 0.9 g NaCl in 10 mL of urine, to which 1.2 mL methanol (as dispersant solvent) and 1 mL of 4-methyl-2-pentanone (as extractant solvent) were added. The absence of a matrix effect allowed quantification of the samples against aqueous standards. Detection limits were 29, 6 and 3 pg mL -1 for TAP, FF and CAP, respectively. Relative standard deviations were calculated to evaluate the intra- and inter-day precision and values lower than 10% were obtained in all cases. The trueness of the method was tested through recovery studies, obtaining satisfactory values (83-104%). Ten urine samples obtained from volunteers were analysed and all of them were free of the studied antibiotics.
(Copyright © 2020 Elsevier B.V. All rights reserved.)

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