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Tytuł:
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Studies on interactions of pentagalloyl glucose, ellagic acid and gallic acid with bovine serum albumin: A spectroscopic analysis.
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Autorzy:
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Zhang L; Key Lab. of Biomass Energy and Material, Nanjing, Jiangsu Province 210042, China; Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, China; Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210042, China. Electronic address: .
Liu Y; Key Lab. of Biomass Energy and Material, Nanjing, Jiangsu Province 210042, China; Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, China; Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210042, China.
Hu X; Key Lab. of Biomass Energy and Material, Nanjing, Jiangsu Province 210042, China; Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, China; Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210042, China.
Xu M; Key Lab. of Biomass Energy and Material, Nanjing, Jiangsu Province 210042, China; Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, China; Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210042, China.
Wang Y; Key Lab. of Biomass Energy and Material, Nanjing, Jiangsu Province 210042, China; Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, China; Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210042, China.
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Źródło:
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Food chemistry [Food Chem] 2020 Sep 15; Vol. 324, pp. 126872. Date of Electronic Publication: 2020 Apr 20.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Barking : Elsevier Applied Science Publishers
Original Publication: Barking, Eng., Applied Science Publishers.
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MeSH Terms:
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Spectrometry, Fluorescence*
Ellagic Acid/*chemistry
Gallic Acid/*chemistry
Hydrolyzable Tannins/*chemistry
Serum Albumin, Bovine/*chemistry
Animals ; Cattle ; Circular Dichroism ; Ellagic Acid/metabolism ; Gallic Acid/metabolism ; Hydrogen-Ion Concentration ; Hydrolyzable Tannins/metabolism ; Protein Binding ; Protein Structure, Secondary ; Serum Albumin, Bovine/metabolism
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Contributed Indexing:
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Keywords: BSA; CD spectra; Ellagic acid; Fluorescence techniques; Gallic acid; PGG
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Substance Nomenclature:
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0 (Hydrolyzable Tannins)
19YRN3ZS9P (Ellagic Acid)
27432CM55Q (Serum Albumin, Bovine)
3UI3K8W93I (pentagalloylglucose)
632XD903SP (Gallic Acid)
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Entry Date(s):
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Date Created: 20200429 Date Completed: 20200731 Latest Revision: 20200731
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Update Code:
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20240105
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DOI:
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10.1016/j.foodchem.2020.126872
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PMID:
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32344347
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Fluorescence techniques and circular dichroism (CD) were utilized to investigate the interactions of a typical gallotannin 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose (PGG) and two simple phenolic compounds ellagic acid (EA) and gallic acid (GA) with bovine serum albumin (BSA). Fluorescence experiments showed that PGG and EA could strongly interact with BSA. The binding constants showed a pH-dependent binding of phenolic acids by BSA. The CD spectra revealed that GA, EA and PGG has slight effect on the secondary structure of BSA at the low concentration (50 μM). However, obvious alterations of the secondary structure of BSA were observed at high concentrations (from 100 μM to 500 μM). The interaction of GA, EA and PGG at high concentrations with BSA caused an unfolding of the secondary structure of BSA. PGG has a greater impact on the secondary structure of BSA when compared with small molecule compounds GA and EA.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
