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Tytuł pozycji:

Prevalence of trypanosomes associated with drug resistance in Shimba Hills, Kwale County, Kenya.

Tytuł :
Prevalence of trypanosomes associated with drug resistance in Shimba Hills, Kwale County, Kenya.
Autorzy :
Kulohoma BW; Centre for Biotechnology and Bioinformatics, University of Nairobi, P.O. Box 30197, Nairobi, 00100, Kenya. .; International Centre of Insect Physiology and Ecology, Nairobi, Kenya. .
Wamwenje SAO; Centre for Biotechnology and Bioinformatics, University of Nairobi, P.O. Box 30197, Nairobi, 00100, Kenya.; International Centre of Insect Physiology and Ecology, Nairobi, Kenya.
Wangwe II; Centre for Biotechnology and Bioinformatics, University of Nairobi, P.O. Box 30197, Nairobi, 00100, Kenya.; International Centre of Insect Physiology and Ecology, Nairobi, Kenya.
Masila N; Kenya Tsetse and Trypanosomiasis Eradication Council (KENTTEC), Kwale County, Kenya.
Mirieri CK; Directorate of Veterinary Services, Vector Regulatory and Zoological Services, Makindu, Kenya.
Wambua L; International Centre of Insect Physiology and Ecology, Nairobi, Kenya.; School of Biological Sciences, University of Nairobi, Nairobi, Kenya.; International Livestock Research Institute, Nairobi, Kenya.
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Źródło :
BMC research notes [BMC Res Notes] 2020 Apr 29; Vol. 13 (1), pp. 234. Date of Electronic Publication: 2020 Apr 29.
Typ publikacji :
Journal Article
Język :
English
Imprint Name(s) :
Original Publication: London : Biomed Central, 2008.
MeSH Terms :
Drug Resistance/*genetics
Nucleoside Transport Proteins/*genetics
Trypanocidal Agents/*therapeutic use
Trypanosoma/*genetics
Trypanosomiasis, African/*epidemiology
Tsetse Flies/*parasitology
Animals ; Female ; Insect Vectors/parasitology ; Kenya ; Male ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Prevalence ; Trypanosoma/isolation & purification ; Trypanosomiasis, African/parasitology
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Grant Information :
RU 2014 GRG-086 Regional Universities Forum for Capacity Building in Agriculture (RUFORUM) grant award
Contributed Indexing :
Keywords: Drug resistance; Infectivity; Prevalence; TbAT1; Trypanosomiasis; Tsetse flies
Substance Nomenclature :
0 (Nucleoside Transport Proteins)
0 (Trypanocidal Agents)
0 (adenosine transporter)
Entry Date(s) :
Date Created: 20200501 Date Completed: 20210208 Latest Revision: 20210208
Update Code :
20210914
PubMed Central ID :
PMC7191804
DOI :
10.1186/s13104-020-05077-3
PMID :
32349785
Czasopismo naukowe
Objective: Animal African trypanosomiasis (AAT) is a life-threatening vector-borne disease, caused by trypanosome parasites, which are principally transmitted by tsetse flies. In Kenya, the prevalence of drug-resistant trypanosomes in endemic regions remains poorly understood. The objective of this study was to establish AAT point prevalence, drug susceptibility of associated trypanosomes, and measure infectivity by multiple AAT mammalian hosts to tsetse flies in Shimba hills, a resource-poor region with high bovine trypanosomiasis prevalence and morbidity rates at the coast of Kenya. We collected tsetse flies using traps (1 Ngu and 2 biconical), and then sorted them on sex and species. Trypanosomes present in tsetse flies were detected by first extracting all genomic DNA, and then performing PCR reactions with established primers of the internal transcribed spacer regions. Polymorphisms associated with trypanocide resistance in the TbAT1 gene were also detected by performing PCR reactions with established primers.
Results: Our findings suggest low trypanosome prevalence (3.7%), low trypanocide resistance, and low infectivity by multiple mammalian hosts to tsetse flies in Shimba hills. We conclude that enhanced surveillance is crucial for informing disease management practices that help prevent the spread of drug-resistant trypanosomiasis.
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