-
Tytuł:
-
Stimulatory effects of Porphyromonas gingivalis GroEL protein on interleukin-6 and interleukin-8 in human osteoblasts.
-
Autorzy:
-
Lin HH; Department of Dentistry, National Yang-Ming University, Taipei, Taiwan.
Lin YW; Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan.
Wu CY; Department of Dentistry, National Yang-Ming University, Taipei, Taiwan; Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan; Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan.
Hsiao FP; Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan.
Lai YL; Department of Dentistry, National Yang-Ming University, Taipei, Taiwan; Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan. Electronic address: .
Hung SL; Department of Dentistry, National Yang-Ming University, Taipei, Taiwan; Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan; Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan. Electronic address: .
-
Źródło:
-
Journal of the Formosan Medical Association = Taiwan yi zhi [J Formos Med Assoc] 2021 Jan; Vol. 120 (1 Pt 1), pp. 150-156. Date of Electronic Publication: 2020 Apr 30.
-
Typ publikacji:
-
Journal Article
-
Język:
-
English
-
Imprint Name(s):
-
Publication: 2003- : Singapore : Formosan Medical Association, Elsevier
Original Publication: Taipei, Taiwan : Formosan Medical Association, [1991-
-
MeSH Terms:
-
Porphyromonas gingivalis*
Humans ; Interleukin-6 ; Interleukin-8/genetics ; Osteoblasts ; p38 Mitogen-Activated Protein Kinases
-
Contributed Indexing:
-
Keywords: GroEL protein; Interleukin-6; Interleukin-8; Osteoblasts; Porphyromonas gingivalis
-
Substance Nomenclature:
-
0 (CXCL8 protein, human)
0 (IL6 protein, human)
0 (Interleukin-6)
0 (Interleukin-8)
EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
-
Entry Date(s):
-
Date Created: 20200504 Date Completed: 20210216 Latest Revision: 20210216
-
Update Code:
-
20240105
-
DOI:
-
10.1016/j.jfma.2020.04.010
-
PMID:
-
32360175
-
Background/purpose: Porphyromonas gingivalis is an oral pathogen associated with periodontal diseases. P. gingivalis GroEL protein is a stimulator of inflammatory cytokines in macrophages. This study inspected effects of P. gingivalis GroEL protein on production of interleukin (IL)-6 and IL-8 by human osteoblasts.
Methods: Viability of GroEL-treated osteoblasts was analyzed with 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide. Secretion of IL-6 and IL-8 was analyzed using the enzyme-linked immunosorbent assay. Levels of mRNA were analyzed using the reverse transcription and real-time polymerase chain reaction. The antioxidant (curcumin), the p38 mitogen-activated protein kinase (MAPK) inhibitor (SB203580) and the c-Jun N-terminal kinase (JNK) inhibitor (SP600125) were employed to elucidate possible signaling pathways involved.
Results: Treatment with GroEL did not affect morphology and viability of osteoblasts. GroEL significantly induced the secretion of IL-6 and IL-8 by osteoblasts in a concentration-dependent pattern. Moreover, the mRNA levels of IL-6 and IL-8 were stimulated by GroEL. The application of SP600125 (10 μM) significantly suppressed the induction of IL-6 and IL-8 by GroEL-treated cells. However, curcumin (20 μM) and SB203580 (20 μM) only down-regulated the stimulatory effects of GroEL on IL-6.
Conclusion: GroEL protein stimulated the inflammatory reaction of osteoblasts, probably through the activation of p38 MAPK or JNK pathway. The findings suggest that P. gingivalis GroEL may influence the immune functions of osteoblasts and endanger the periodontal health.
(Copyright © 2020 Formosan Medical Association. Published by Elsevier B.V. All rights reserved.)
