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Tytuł:
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PC4 serves as a negative regulator of skin wound healing in mice.
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Autorzy:
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Liao F; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Chen L; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Luo P; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Jiang Z; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Chen Z; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Wang Z; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Zhang C; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Wang Y; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
He J; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Wang Q; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.; Institute of Clinical Medicine, Southwest Medical University, 646000 Luzhou, China.
Wang Y; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Liu L; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.; Department of Toxicology, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, 550025 Guiyang, China.
Huang Y; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.; Department of Toxicology, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, 550025 Guiyang, China.
Wang H; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.; Institute of Clinical Medicine, Southwest Medical University, 646000 Luzhou, China.
Jiang Q; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.; Institute of Clinical Medicine, Southwest Medical University, 646000 Luzhou, China.
Luo M; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.; Department of Toxicology, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, 550025 Guiyang, China.
Gan Y; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Liu Y; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Wang Y; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Wu J; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Xie W; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Cheng Z; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Dai Y; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Li J; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Liu Z; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Yang F; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Shi C; Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University (Army Medical University), Chongqing 400038, China.
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Źródło:
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Burns & trauma [Burns Trauma] 2020 May 05; Vol. 8, pp. tkaa010. Date of Electronic Publication: 2020 May 05 (Print Publication: 2020).
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: 2020- : Oxford : Oxford University Press
Original Publication: [Mumbai] : published by Wolters Kluwer Health - Medknow, Institute of Burn Research, Southwest Hospital, Third Military Medical University, China ; [2013]-
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References:
-
Cell. 1994 Aug 12;78(3):525-34. (PMID: 8062392)
Cell Tissue Res. 2016 Aug;365(2):343-56. (PMID: 26987821)
J Am Chem Soc. 2014 Feb 26;136(8):2948-51. (PMID: 24524683)
PLoS Genet. 2015 Mar 30;11(3):e1005105. (PMID: 25822411)
Nucleic Acids Res. 2016 Feb 18;44(3):1203-15. (PMID: 26740582)
Nat Cell Biol. 2019 Jan;21(1):18-24. (PMID: 30602767)
J Mol Cell Cardiol. 2016 Feb;91:151-9. (PMID: 26776318)
In Vitro Cell Dev Biol Anim. 2007 Sep-Oct;43(8-9):290-6. (PMID: 17876677)
Exp Cell Res. 1998 Feb 25;239(1):1-10. (PMID: 9511718)
Cell Commun Signal. 2018 Nov 16;16(1):82. (PMID: 30445978)
Brain Res. 1996 Jan 29;707(2):293-7. (PMID: 8919308)
Cell. 1994 Aug 12;78(3):513-23. (PMID: 8062391)
Mol Cell Biol. 2006 Nov;26(22):8303-15. (PMID: 16982701)
Sci Transl Med. 2014 Dec 3;6(265):265sr6. (PMID: 25473038)
EMBO J. 2005 Mar 9;24(5):1009-20. (PMID: 15692559)
J Invest Dermatol. 2019 Mar;139(3):702-711. (PMID: 30393083)
Oncogene. 2016 Feb 11;35(6):761-70. (PMID: 25961912)
Cell Commun Signal. 2019 Apr 16;17(1):36. (PMID: 30992017)
Curr Genet. 2017 Dec;63(6):1023-1035. (PMID: 28567479)
Cancer Gene Ther. 2012 Oct;19(10):690-6. (PMID: 22918472)
Physiol Rev. 2019 Jan 1;99(1):665-706. (PMID: 30475656)
Plast Reconstr Surg. 2015 Nov;136(5):1004-13. (PMID: 26505703)
Mol Cell. 1998 Apr;1(5):749-57. (PMID: 9660958)
Nature. 2018 Sep;561(7722):243-247. (PMID: 30185909)
J Mol Biol. 2010 Mar 19;397(1):1-12. (PMID: 20080105)
Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14265-70. (PMID: 19706510)
J Cell Biochem. 2019 Jun;120(6):10847-10854. (PMID: 30681184)
Sci Rep. 2015 Mar 05;5:8789. (PMID: 25739870)
Chem Commun (Camb). 2015 Apr 28;51(33):7242-4. (PMID: 25813861)
J Neurol Sci. 2014 Nov 15;346(1-2):293-8. (PMID: 25262015)
Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2192-7. (PMID: 9482861)
Genes Cells. 2014 Dec;19(12):879-90. (PMID: 25308091)
Am J Cancer Res. 2019 Apr 01;9(4):682-698. (PMID: 31105996)
Burns Trauma. 2019 Nov 8;7:31. (PMID: 31720300)
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Contributed Indexing:
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Keywords: Migration, Positive cofactor 4; PC4; Proliferation; Skin; Wound healing
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Entry Date(s):
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Date Created: 20200507 Latest Revision: 20220414
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Update Code:
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20240105
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PubMed Central ID:
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PMC7198317
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DOI:
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10.1093/burnst/tkaa010
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PMID:
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32373645
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Background: Human positive cofactor 4 (PC4) was initially characterized as a multifunctional transcriptional cofactor, but its role in skin wound healing is still unclear. The purpose of this study was to explore the role of PC4 in skin wound healing through PC4 knock-in mouse model.
Methods: A PC4 knock-in mouse model (PC4 +/+ ) with a dorsal full-thickness wound was used to investigate the biological functions of PC4 in skin wound healing. Quantitative PCR, Western blot analysis and immunohistochemistry were performed to evaluate the expression of PC4; Sirius red staining and immunofluorescence were performed to explore the change of collagen deposition and angiogenesis. Proliferation and apoptosis were detected using Ki67 staining and TUNEL assay. Primary dermal fibroblasts were isolated from mouse skin to perform cell scratch experiments, cck-8 assay and colony formation assay.
Results: The PC4 +/+ mice were fertile and did not display overt abnormalities but showed an obvious delay in cutaneous healing of dorsal skin. Histological staining showed insufficient re-epithelialization, decreased angiogenesis and collagen deposition, increased apoptosis and decreased cell proliferation in PC4 +/+ skin. Our data also showed decreased migration rate and proliferation ability in cultured primary fibroblasts from PC4 +/+ mice in vitro .
Conclusions: This study suggests that PC4 might serve as a negative regulator of skin wound healing in mice.
(© The Author(s) 2020. Published by Oxford University Press.)
