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Tytuł:
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Bioactive structural basis of proteoglycans from Sarcandra glabra based on spectrum-effect relationship.
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Autorzy:
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Sun X; Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, PR China.
Zhao Q; Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, PR China.
Si Y; Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, PR China.
Li K; Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, PR China.
Zhu J; Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, PR China.
Gao X; Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, PR China. Electronic address: .
Liu W; Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, PR China. Electronic address: .
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Źródło:
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Journal of ethnopharmacology [J Ethnopharmacol] 2020 Sep 15; Vol. 259, pp. 112941. Date of Electronic Publication: 2020 May 07.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Limerick : Elsevier Sequoia
Original Publication: Lausanne, Elsevier Sequoia.
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MeSH Terms:
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Magnoliopsida*/chemistry
Spectroscopy, Fourier Transform Infrared*
Antineoplastic Agents, Phytogenic/*pharmacology
Antioxidants/*pharmacology
Cell Proliferation/*drug effects
Neoplasms/*drug therapy
Plant Extracts/*pharmacology
Proteoglycans/*pharmacology
Antineoplastic Agents, Phytogenic/isolation & purification ; Antioxidants/isolation & purification ; Cell Line, Tumor ; Dose-Response Relationship, Drug ; Humans ; Inhibitory Concentration 50 ; Molecular Structure ; Molecular Weight ; Neoplasms/pathology ; Plant Extracts/isolation & purification ; Proteoglycans/isolation & purification ; Structure-Activity Relationship
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Contributed Indexing:
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Keywords: Fingerprint; Proteoglycans; Sarcandra glabra; Spectrum-effect relationship
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Substance Nomenclature:
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0 (Antineoplastic Agents, Phytogenic)
0 (Antioxidants)
0 (Plant Extracts)
0 (Proteoglycans)
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Entry Date(s):
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Date Created: 20200512 Date Completed: 20210225 Latest Revision: 20210225
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Update Code:
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20240105
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DOI:
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10.1016/j.jep.2020.112941
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PMID:
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32389856
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Ethnopharmacological Relevance: Proteoglycans are one of the active ingredients of great importance in Sarcandra glabra. The biological activities of proteoglycans extracted from Sarcandra glabra including suppressing tumor growth and antioxidant activity were studied. However, raw materials from different regions may cause differences in the activity of natural extracts, especially for bioactive biomacromolecules. Conventional identification of S.glabra cannot accurately reflect the distinguishing relationship between internal components and the pharmacological activity. The identification of biologically active structures was obtained by constructing multiple fingerprint and spectrum-effect relationship.
Aim of the Study: To evaluate the bioactive structural basis of proteoglycans from S.glabra based on spectrum-effect relationship and chemometric methods.
Materials and Methods: Multiple fingerprinting including HPSEC, PMP-HPLC, and FT-IR of proteoglycans was established from 18 batches of samples based on the structural characteristics. Both antitumor activity and antioxidant activity were determined. Mathematical analysis was used to analyze the spectrum-effect relationship.
Results: PCA results showed monosaccharides including Xly, Rha, and GlcA, carboxyl group in acidic sugars, peptide bond in proteins, and methylene groups could be used as markers for distinguishing the samples from different sources. The results of the spectrum-effect relationship analysis indicated that the bioactive markers of inhibitory activity on MG63 and U2OS cells by PLS-DA were related to GlcA, Xyl, Fuc, β-glycosidic bonds, peptide linkage, and methylene groups. Markers composing monosaccharide for antioxidant activity were Xyl, GlcA, and GlcN. Meanwhile, the group markers were pyranose ring, carboxyl group, peptide linkage, and methylene structure.
Conclusions: The material basis that affects the pharmacological efficacy could be found according to the spectrum-effect relationship analysis. This study could lay a foundation for further exploring the relationship between structural characteristics and pharmacodynamics of macromolecular glycoconjugates in Traditional Chinese Medicine.
Competing Interests: Declaration of competing interest All authors declare that there are no conflicts of interest.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
