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Tytuł pozycji:

Washing-free centrifugal microchip fluorescence immunoassay for rapid and point-of-care detection of protein.

Tytuł :
Washing-free centrifugal microchip fluorescence immunoassay for rapid and point-of-care detection of protein.
Autorzy :
Lin Q; Department of Chemistry, Institutes of Biomedical Sciences, Fudan University, Shanghai, 200433, PR China.
Wu J; Shanghai Suxin Biotechnology Co. Ltd, and IgeneTec Diagnostic Products Co. Ltd., Shanghai, 201318, PR China.
Fang X; Department of Chemistry, Institutes of Biomedical Sciences, Fudan University, Shanghai, 200433, PR China. Electronic address: .
Kong J; Department of Chemistry, Institutes of Biomedical Sciences, Fudan University, Shanghai, 200433, PR China. Electronic address: .
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Źródło :
Analytica chimica acta [Anal Chim Acta] 2020 Jun 29; Vol. 1118, pp. 18-25. Date of Electronic Publication: 2020 Apr 13.
Typ publikacji :
Journal Article
Język :
English
Imprint Name(s) :
Publication: Amsterdam : Elsevier
Original Publication: Amsterdam.
MeSH Terms :
Centrifugation*
Immunoassay*
Lab-On-A-Chip Devices*
Point-of-Care Systems*
Procalcitonin/*analysis
Humans
Contributed Indexing :
Keywords: Fluorescence immunoassay; Point-of-care detection; Protein; Washing-free centrifugal microchip
Substance Nomenclature :
0 (Procalcitonin)
Entry Date(s) :
Date Created: 20200519 Date Completed: 20201229 Latest Revision: 20201229
Update Code :
20210210
DOI :
10.1016/j.aca.2020.04.031
PMID :
32418600
Czasopismo naukowe
Simplifying the procedure of immunoassay is still a challenge due to problems such as multiple washing processes, complicated chemical modification and expensive cost. In this study, we developed a portable centrifugal microchip fluorescence immunoassay for washing-free, rapid, quantitative and point-of-care (POC) detection of protein. The designed microchip was fabricated by polycarbonate and assembled by double-sided adhesive tape using injecting molding with high scalability and low cost. The centrifugal strategy is capable of washing-out the bio-fluid and improving signal-to-noise ratio. Matrix nano-spotting method was employed to facilitate satisfactory immunological binding sites with the advantage of high capture efficiency and reproducibility. The proposed approach was capable of sensitively detecting procalcitonin (PCT) with a wide dynamic ranging from 0.10 ng/mL to 70.00 ng/mL within 10 min. Furthermore, this novel integrated diagnostic tool was successfully applied to detect PCT in 101 clinical samples with good consistency with Roche's method, indicating its attractive practical application capability. With favorable simplicity, rapidity, low cost and excellent analytical performance, our method holds great promise for POC diagnostics of proteins.
(Copyright © 2020. Published by Elsevier B.V.)

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