Structural basis for assembly and function of a diatom photosystem I-light-harvesting supercomplex.

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Tytuł:: Structural basis for assembly and function of a diatom photosystem I-light-harvesting supercomplex.
Autorzy:: Nagao R; Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University, Okayama, 700-8530, Japan.
Kato K; Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University, Okayama, 700-8530, Japan.
Ifuku K; Graduate School of Biostudies, Kyoto University, Kyoto, 606-8502, Japan.
Suzuki T; Biomolecular Characterization Unit, RIKEN Center for Sustainable Resource Science, Saitama, 351-0198, Japan.
Kumazawa M; Faculty of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.
Uchiyama I; National Institute for Basic Biology, National Institutes of Natural Sciences, Aichi, 444-8585, Japan.
Kashino Y; Graduate School of Life Science, University of Hyogo, Hyogo, 678-1297, Japan.
Dohmae N; Biomolecular Characterization Unit, RIKEN Center for Sustainable Resource Science, Saitama, 351-0198, Japan.
Akimoto S; Graduate School of Science, Kobe University, Hyogo, 657-8501, Japan.
Shen JR; Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University, Okayama, 700-8530, Japan.
Miyazaki N; Institute for Protein Research, Osaka University, Osaka, 565-0871, Japan. .; Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Ibaraki, 305-8577, Japan. .
Akita F; Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University, Okayama, 700-8530, Japan. fusamichi_.; Japan Science and Technology Agency, PRESTO, Saitama, 332-0012, Japan. fusamichi_.
Źródło:: Nature communications [Nat Commun] 2020 May 18; Vol. 11 (1), pp. 2481. Date of Electronic Publication: 2020 May 18.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: [London] : Nature Pub. Group
MeSH Terms:: Diatoms/*metabolism
Light-Harvesting Protein Complexes/*chemistry
Light-Harvesting Protein Complexes/*metabolism
Photosystem I Protein Complex/*chemistry
Photosystem I Protein Complex/*metabolism
Chlorophyll/metabolism ; Chlorophyll Binding Proteins/chemistry ; Chlorophyll Binding Proteins/ultrastructure ; Energy Transfer ; Light-Harvesting Protein Complexes/ultrastructure ; Models, Molecular ; Photosystem I Protein Complex/ultrastructure ; Protein Binding ; Protein Subunits/metabolism ; Structure-Activity Relationship
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Substance Nomenclature:: 0 (Chlorophyll Binding Proteins)
0 (Light-Harvesting Protein Complexes)
0 (Photosystem I Protein Complex)
0 (Protein Subunits)
1406-65-1 (Chlorophyll)
Entry Date(s):: Date Created: 20200520 Date Completed: 20200812 Latest Revision: 20210518
Update Code:: 20240105
PubMed Central ID:: PMC7235021
DOI:: 10.1038/s41467-020-16324-3
PMID:: 32424145
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Photosynthetic light-harvesting complexes (LHCs) play a pivotal role in collecting solar energy for photochemical reactions in photosynthesis. One of the major LHCs are fucoxanthin chlorophyll a/c-binding proteins (FCPs) present in diatoms, a group of organisms having important contribution to the global carbon cycle. Here, we report a 2.40-Å resolution structure of the diatom photosystem I (PSI)-FCPI supercomplex by cryo-electron microscopy. The supercomplex is composed of 16 different FCPI subunits surrounding a monomeric PSI core. Each FCPI subunit showed different protein structures with different pigment contents and binding sites, and they form a complicated pigment-protein network together with the PSI core to harvest and transfer the light energy efficiently. In addition, two unique, previously unidentified subunits were found in the PSI core. The structure provides numerous insights into not only the light-harvesting strategy in diatom PSI-FCPI but also evolutionary dynamics of light harvesters among oxyphototrophs.

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