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Tytuł:
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Isolation and identification of potential probiotic Lactobacillus species from feces of infants in southwest Iran.
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Autorzy:
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Jomehzadeh N; Abadan Faculty of Medical Sciences, Abadan, Iran; Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Javaherizadeh H; Abuzar Children's Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Alimentary Tract Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Electronic address: .
Amin M; Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Saki M; Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Electronic address: .
Al-Ouqaili MTS; Department of Microbiology, College of Medicine, University of Anbar, Iraq.
Hamidi H; Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Seyedmahmoudi M; Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Gorjian Z; Abadan Faculty of Medical Sciences, Abadan, Iran.
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Źródło:
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International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases [Int J Infect Dis] 2020 Jul; Vol. 96, pp. 524-530. Date of Electronic Publication: 2020 May 19.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Hamilton, ON : Elsevier
Original Publication: Hamilton, ON : Decker, c1996-
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MeSH Terms:
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Lactobacillus/*isolation & purification
Probiotics/*chemistry
Anti-Bacterial Agents/pharmacology ; Bacterial Adhesion ; Child, Preschool ; Feces/microbiology ; Female ; Humans ; Infant ; Iran ; Lactobacillus/classification ; Lactobacillus/genetics ; Lactobacillus/physiology ; Male ; Probiotics/classification ; Probiotics/isolation & purification ; Probiotics/pharmacology ; RNA, Ribosomal, 16S/genetics ; Yersinia enterocolitica/drug effects ; Yersinia enterocolitica/growth & development
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Contributed Indexing:
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Keywords: Adhesion; Enteropathogens; Lactobacillus; Probiotic
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Substance Nomenclature:
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0 (Anti-Bacterial Agents)
0 (RNA, Ribosomal, 16S)
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Entry Date(s):
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Date Created: 20200523 Date Completed: 20200908 Latest Revision: 20200908
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Update Code:
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20240105
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DOI:
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10.1016/j.ijid.2020.05.034
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PMID:
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32439543
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Objectives: To evaluate the potential probiotic properties of Lactobacillus strains isolated from feces of infants and also to determine their antimicrobial activity against some enteropathogenic bacteria.
Methods: The Fecal samples were prepared from 120 infants aged less than 24 months. In total, 105 Lactobacillus strains were identified by phenotypic tests. Thirty isolates were randomly selected to study their potential probiotic properties. These isolates were examined for resistance to acid (pH: 2.5, 2 h) and bile (oxgall 0.3%, 8 h), adhesion to HT-29 cells, antibiotic susceptibility, and antimicrobial activities.
Results: On basis of 16S rRNA sequencing, 30 isolates identified as Lactobacillus fermentum (n = 11; 36.7%), Lactobacillus plantarum (n = 9; 30%), Lactobacillus rhamnosus (n = 6; 20%), and Lactobacillus paracasei (n = 4; 13.3%). All tested strains survived at acid and bile conditions. Six Lactobacillus strains revealed high adherence to HT-29 cells. Three strains including the L. fermentum (N2, N7), and the L. plantarum (N20) showed good probiotic potential and inhibited the growth of Yersinia enterocolitica ATCC 23715, Shigella flexneri ATCC 12022, Salmonella enterica ATCC 9270, and enteropathogenic Escherichia coli (EPEC) ATCC 43887. The antibiotic resistance test showed that all the isolates were susceptible to tetracycline, and chloramphenicol.
Conclusions: Lactobacillus strains like L. fermentum (N2, N7), and the L. plantarum (N20), could be potential probiotic, but further in vitro and in vivo studies on these probiotic strains are still required.
(Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
