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Tytuł pozycji:

MiR-34a suppresses the proliferation and invasion of gastric cancer by modulating PDL1 in the immune microenvironment.

Tytuł :
MiR-34a suppresses the proliferation and invasion of gastric cancer by modulating PDL1 in the immune microenvironment.
Autorzy :
Yong H; Department of Oncology, The Affiliated Huai'an Hospital of Xuzhou Medical University and the Second People's Hospital of Huai'an, Huai'an, 223002, China; Cancer Institute, Xuzhou Medical University, 84 West Huaihai Road, Xuzhou, 221002, China.
Fu J; Department of Intensive Care Unit, The Affiliated Huai'an Hospital of Xuzhou Medical University and the Second People's Hospital of Huai'an, Huai'an, 223002, China.
Gao G; Department of Oncology, The Affiliated Huai'an Hospital of Xuzhou Medical University and the Second People's Hospital of Huai'an, Huai'an, 223002, China.
Shi H; Department of Oncology, The Affiliated Huai'an Hospital of Xuzhou Medical University and the Second People's Hospital of Huai'an, Huai'an, 223002, China.
Zheng D; Department of Nephrology, The Affiliated Huai'an Hospital of Xuzhou Medical University and the Second People's Hospital of Huai'an, Huaian, 223002, China. Electronic address: .
Zhou X; Department of Oncology, The Affiliated Huai'an Hospital of Xuzhou Medical University and the Second People's Hospital of Huai'an, Huai'an, 223002, China. Electronic address: .
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Źródło :
Molecular and cellular probes [Mol Cell Probes] 2020 Oct; Vol. 53, pp. 101601. Date of Electronic Publication: 2020 May 20.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
Język :
English
Imprint Name(s) :
Original Publication: London ; New York : Academic Press, c1987-
Contributed Indexing :
Keywords: Gastric cancer*; Immunotherapy*; PDL1*; miR-34a*
Entry Date(s) :
Date Created: 20200524 Latest Revision: 20201106
Update Code :
20201218
DOI :
10.1016/j.mcp.2020.101601
PMID :
32445780
Czasopismo naukowe
Objectives: As one of the most serious malignant carcinomas that threaten the life of sufferers constantly, gastric cancer has attracted a lot of interest among researchers. miR-34a, a member of hundreds of microRNAs (miRNAs), has been elucidated to exert a suppressive role in gastric cancer tumorgenesis based on previous extensive studies. Our study was performed with the aim to explore the functional effects of miR-34a and its predictive target programmed death ligand 1 (PDL1) in gastric cancer development.
Methods: We employed reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western Blot analysis to investigate the regulatory effect of miR-34a on PDL1 mRNA and the corresponding protein expression. The CCK-8 and colony formation assays were used to validate the influence of the combination of miR-34a and PDL1 on the proliferation of gastric tumor cells. Meanwhile, the migration and invasion of gastric tumor cells were measured using Transwell assay.
Results: PDL1 was targeted and negatively modulated by miR-34a. In addition, the re-expression of miR-34a suppressed the proliferation as well as the migration and invasion of gastric tumor cells, whereas PDL1 reduced the aforementioned inhibitory effect.
Conclusions: PDL1 is the downstream gene of miR-34a, which can act as an anti-oncogene in gastric cancer. The miR-34a/PDL1 axis might provide a promising anticancer therapeutic approach for the clinical diagnosis, treatment, and prognosis of gastric cancer.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)

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