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Tytuł:
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Purification and identification of novel antioxidant peptides from watermelon seed protein hydrolysates and their cytoprotective effects on H 2 O 2 -induced oxidative stress.
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Autorzy:
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Wen C; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: .
Zhang J; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: .
Feng Y; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Duan Y; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Institute of Food Physical Processing, Jiangsu University, Zhenjiang 212013, China. Electronic address: .
Ma H; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; Institute of Food Physical Processing, Jiangsu University, Zhenjiang 212013, China. Electronic address: .
Zhang H; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: .
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Źródło:
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Food chemistry [Food Chem] 2020 Oct 15; Vol. 327, pp. 127059. Date of Electronic Publication: 2020 May 15.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Barking : Elsevier Applied Science Publishers
Original Publication: Barking, Eng., Applied Science Publishers.
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MeSH Terms:
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Citrullus/*chemistry
Hydrogen Peroxide/*pharmacology
Oxidative Stress/*drug effects
Peptides/*chemistry
Protein Hydrolysates/*chemistry
Amino Acid Sequence ; Antioxidants/chemistry ; Antioxidants/isolation & purification ; Antioxidants/pharmacology ; Chromatography, High Pressure Liquid ; Citrullus/metabolism ; Hep G2 Cells ; Humans ; Oxidation-Reduction ; Peptides/isolation & purification ; Peptides/pharmacology ; Plant Proteins/metabolism ; Protective Agents/chemistry ; Protective Agents/isolation & purification ; Protective Agents/pharmacology ; Reactive Oxygen Species/metabolism ; Seeds/chemistry ; Seeds/metabolism ; Structure-Activity Relationship ; Tandem Mass Spectrometry
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Contributed Indexing:
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Keywords: Antioxidant activity; Cytoprotective effect; In silico analysis; Structure-activity relationship; Watermelon seed peptides
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Substance Nomenclature:
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0 (Antioxidants)
0 (Peptides)
0 (Plant Proteins)
0 (Protective Agents)
0 (Protein Hydrolysates)
0 (Reactive Oxygen Species)
BBX060AN9V (Hydrogen Peroxide)
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Entry Date(s):
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Date Created: 20200525 Date Completed: 20200831 Latest Revision: 20200831
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Update Code:
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20240105
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DOI:
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10.1016/j.foodchem.2020.127059
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PMID:
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32447138
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The aim of this study was to purify and identify antioxidant peptides from watermelon seed protein hydrolysates (WSPHs-I: Mw < 1 kDa) and further evaluate their cytoprotective effects against H 2 O 2 -induced oxidative stress in HepG2 cells. After purification by Sephadex G-15 and semi-preparative reversed-phase high performance liquid chromatography (RP-HPLC), five peptides, RDPEER (P1), KELEEK (P2), DAAGRLQE (P3), LDDDGRL (P4), and GFAGDDAPRA (P5) were sequenced by LC-MS/MS and synthesized with solid-phase synthesis method. These peptides showed desirable 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity (IC 50 : 0.216 ± 0.01-0.435 ± 0.03), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging capacity (IC 50 : 0.54 ± 0.02-1.23 ± 0.03), and oxygen radical absorbance capacity (ORAC) (82.36 ± 1.2-130.67 ± 2.2 μM TE/mg). Among them, peptide P1 exhibited the strongest antioxidant capacity. Moreover, the results suggested that peptide P1 may protect HepG2 cells from H 2 O 2 -induced oxidative damage by significantly inhibiting reactive oxygen species (ROS), [Ca 2+ ]i, malondialdehyde (MDA) levels and increasing antioxidative enzyme activities.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
