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Tytuł:
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The netrin receptor UNC-40/DCC assembles a postsynaptic scaffold and sets the synaptic content of GABA A receptors.
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Autorzy:
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Zhou X; Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR 5310, INSERM U 1217, Institut NeuroMyoGène, 69008, Lyon, France.
Gueydan M; Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR 5310, INSERM U 1217, Institut NeuroMyoGène, 69008, Lyon, France.
Jospin M; Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR 5310, INSERM U 1217, Institut NeuroMyoGène, 69008, Lyon, France.
Ji T; Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR 5310, INSERM U 1217, Institut NeuroMyoGène, 69008, Lyon, France.; Instrumental analysis center, Shanghai Jiao Tong University, 200240, Shanghai, China.
Valfort A; Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR 5310, INSERM U 1217, Institut NeuroMyoGène, 69008, Lyon, France.; Center for clinical pharmacology, Saint Louis College of Pharmacy, 2 Pharmacy Place, Saint-Louis, MO 63110, USA.
Pinan-Lucarré B; Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR 5310, INSERM U 1217, Institut NeuroMyoGène, 69008, Lyon, France. .
Bessereau JL; Univ Lyon, Université Claude Bernard Lyon 1, CNRS UMR 5310, INSERM U 1217, Institut NeuroMyoGène, 69008, Lyon, France. .
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Źródło:
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Nature communications [Nat Commun] 2020 May 29; Vol. 11 (1), pp. 2674. Date of Electronic Publication: 2020 May 29.
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Typ publikacji:
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Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: [London] : Nature Pub. Group
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MeSH Terms:
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Caenorhabditis elegans/*metabolism
Caenorhabditis elegans Proteins/*metabolism
Cell Adhesion Molecules/*metabolism
Receptors, Cell Surface/*metabolism
Receptors, GABA-A/*metabolism
Synaptic Transmission/*physiology
Animals ; Axon Guidance/physiology ; Cell Adhesion Molecules, Neuronal/metabolism ; Cytoskeletal Proteins/metabolism ; Helminth Proteins/metabolism ; Membrane Proteins/metabolism ; Nerve Tissue Proteins/metabolism ; Neuromuscular Junction/metabolism ; Synapses/physiology
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References:
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Grant Information:
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P40 OD010440 United States OD NIH HHS
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Substance Nomenclature:
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0 (Caenorhabditis elegans Proteins)
0 (Cell Adhesion Molecules)
0 (Cell Adhesion Molecules, Neuronal)
0 (Cytoskeletal Proteins)
0 (Helminth Proteins)
0 (Lin-2 protein, C elegans)
0 (MADD-4 protein, C elegans)
0 (Membrane Proteins)
0 (Nerve Tissue Proteins)
0 (Receptors, Cell Surface)
0 (Receptors, GABA-A)
0 (UNC-40 protein, C elegans)
0 (neuroligin 1)
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Entry Date(s):
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Date Created: 20200531 Date Completed: 20200824 Latest Revision: 20210529
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Update Code:
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20240104
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PubMed Central ID:
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PMC7260190
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DOI:
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10.1038/s41467-020-16473-5
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PMID:
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32471987
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Increasing evidence indicates that guidance molecules used during development for cellular and axonal navigation also play roles in synapse maturation and homeostasis. In C. elegans the netrin receptor UNC-40/DCC controls the growth of dendritic-like muscle cell extensions towards motoneurons and is required to recruit type A GABA receptors (GABA A Rs) at inhibitory neuromuscular junctions. Here we show that activation of UNC-40 assembles an intracellular synaptic scaffold by physically interacting with FRM-3, a FERM protein orthologous to FARP1/2. FRM-3 then recruits LIN-2, the ortholog of CASK, that binds the synaptic adhesion molecule NLG-1/Neuroligin and physically connects GABA A Rs to prepositioned NLG-1 clusters. These processes are orchestrated by the synaptic organizer CePunctin/MADD-4, which controls the localization of GABA A Rs by positioning NLG-1/neuroligin at synapses and regulates the synaptic content of GABA A Rs through the UNC-40-dependent intracellular scaffold. Since DCC is detected at GABA synapses in mammals, DCC might also tune inhibitory neurotransmission in the mammalian brain.