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Tytuł:
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Regulation of poly ADP-ribosylation of VEGF by an interplay between PARP-16 and TNKS-2.
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Autorzy:
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Kunhiraman H; Department of Biochemistry and Molecular Biology, Central University of Kerala, Tejaswini Hills, Periya, Kasargod, Kerala, India.; Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, School of Medicine, John Hopkins University, 1650 Orleans St., Baltimore, MD, 21231, USA.; Cancer and Blood Disorder Institute, Johns Hopkins All Children's Hospital, 600 5th St. South, St. Petersburg, FL, 33701, USA.
Ramachandran V; Department of Biochemistry and Molecular Biology, Central University of Kerala, Tejaswini Hills, Periya, Kasargod, Kerala, India.
Edatt L; Department of Biochemistry and Molecular Biology, Central University of Kerala, Tejaswini Hills, Periya, Kasargod, Kerala, India.
Sameer Kumar VB; Department of Biochemistry and Molecular Biology, Central University of Kerala, Tejaswini Hills, Periya, Kasargod, Kerala, India. .
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Źródło:
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Molecular and cellular biochemistry [Mol Cell Biochem] 2020 Aug; Vol. 471 (1-2), pp. 15-27. Date of Electronic Publication: 2020 May 30.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: New York : Springer
Original Publication: The Hague, Dr. W. Junk B. V. Publishers.
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MeSH Terms:
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Poly ADP Ribosylation*
Protein Processing, Post-Translational*
Poly(ADP-ribose) Polymerases/*metabolism
Tankyrases/*metabolism
Vascular Endothelial Growth Factor A/*metabolism
Animals ; Cells, Cultured ; Chick Embryo ; Humans ; Poly(ADP-ribose) Polymerases/genetics ; Tankyrases/genetics
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Grant Information:
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SB/YS/LS-367/2013 DST-SERB, India
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Contributed Indexing:
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Keywords: ADP-ribosylation; Angiogenesis; PARP-16; TNKS-2; VEGF
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Substance Nomenclature:
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0 (VEGFA protein, human)
0 (Vascular Endothelial Growth Factor A)
EC 2.4.2.30 (PARP16 protein, human)
EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
EC 2.4.2.30 (TNKS2 protein, human)
EC 2.4.2.30 (Tankyrases)
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Entry Date(s):
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Date Created: 20200531 Date Completed: 20210202 Latest Revision: 20210202
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Update Code:
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20240104
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DOI:
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10.1007/s11010-020-03746-2
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PMID:
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32472322
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The biological activity of vascular endothelial growth factor (VEGF), the major cytokine regulating the process of angiogenesis is tightly controlled at multiple levels including processes involving post-translational modification such as ADP-ribosylation and glycosylation. ADP-ribosylation is a reversible NAD + -dependent modification, catalyzed by poly ADP-ribose polymerase (PARP) or ADP-ribosyl transferase (ADPRTs) and has been reported by us and others as a modification that reduces the biological activity of VEGF. The factors responsible for any such modification should occur in the secretory pathway, i.e., in the endoplasmic reticulum and Golgi. Our investigation carried out in this direction revealed that ADP-ribosylation of VEGF requires the interplay between members of poly ADP-ribose polymerase (PARP) family in the secretory pathway, viz., ER associated PARP-16 and Golgi associated Tankyrase-2 (TNKS-2). The data presented in this manuscript suggest that PARP-16 catalysis the priming mono ADP-ribosylation of VEGF which is a prerequisite for poly ADP-ribosylation of VEGF by TNKS-2.