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Tytuł pozycji:

Combination of multicolor flow cytometry for circulating lymphoma cells and tests for the RHOA G17V and IDH2 R172 hot-spot mutations in plasma cell-free DNA as liquid biopsy for the diagnosis of angioimmunoblastic T-cell lymphoma.

Tytuł:
Combination of multicolor flow cytometry for circulating lymphoma cells and tests for the RHOA hot-spot mutations in plasma cell-free DNA as liquid biopsy for the diagnosis of angioimmunoblastic T-cell lymphoma.
Autorzy:
Hayashida M; Tenri Institute of Medical Research, Tenri, Japan.; Department of Clinical Laboratory and Biomedical Sciences, Osaka University Graduate School of Medicine, Suita, Japan.
Maekawa F; Tenri Institute of Medical Research, Tenri, Japan.
Chagi Y; Tenri Institute of Medical Research, Tenri, Japan.
Iioka F; Department of Hematology, Tenri Hospital, Tenri, Japan.
Kobashi Y; Department of Diagnostic Surgical Pathology, Tenri Hospital, Tenri, Japan.
Watanabe M; Department of Clinical Laboratory and Biomedical Sciences, Osaka University Graduate School of Medicine, Suita, Japan.
Ohno H; Tenri Institute of Medical Research, Tenri, Japan.; Department of Hematology, Tenri Hospital, Tenri, Japan.
Źródło:
Leukemia & lymphoma [Leuk Lymphoma] 2020 Oct; Vol. 61 (10), pp. 2389-2398. Date of Electronic Publication: 2020 May 30.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Publication: [Philadelphia, PA] : Taylor & Francis
Original Publication: Chur ; New York : London, UK : Harwood Academic Publishers ; Distributed by STBS, 1989-
MeSH Terms:
Cell-Free Nucleic Acids*
Immunoblastic Lymphadenopathy*
Lymphoma, T-Cell*/diagnosis
Lymphoma, T-Cell*/genetics
Biomarkers, Tumor/analysis ; Flow Cytometry ; Humans ; Liquid Biopsy ; Mutation ; Plasma/chemistry ; Plasma/metabolism ; rhoA GTP-Binding Protein/genetics ; rhoA GTP-Binding Protein/metabolism
Contributed Indexing:
Keywords: Angioimmunoblastic T-cell lymphoma; IDH2R172 mutations; RHOAG17V mutation; cell-free DNA; liquid biopsy; multicolor flow cytometry
Substance Nomenclature:
0 (Biomarkers, Tumor)
0 (Cell-Free Nucleic Acids)
124671-05-2 (RHOA protein, human)
EC 3.6.5.2 (rhoA GTP-Binding Protein)
Entry Date(s):
Date Created: 20200602 Date Completed: 20210427 Latest Revision: 20210427
Update Code:
20240104
DOI:
10.1080/10428194.2020.1768382
PMID:
32476550
Czasopismo naukowe
We applied two-step multicolor flow cytometry (FCM) for circulating lymphoma cells in the blood of 20 patients with angioimmunoblastic T-cell lymphoma (AITL) and confirmed neoplastic T-cells in all. Eleven exhibited dim expression of CD3 and 7 lost its expression. The proportion of CD10 + lymphoma cells ranged widely from 0 to 100%, with a median of 15.7%. Ten patients demonstrated expansion of a single T-cell receptor β-chain repertoire. Lymphoma cells comprised 0.01 to 18.22% (median, 0.26%) of white cells and the absolute numbers ranged from 0.5 to 1491.6 cells (median, 29.3 cells) per microliter of blood. We next found that 14 (70%) and 3 (15%) patients carried RHOA G17V and IDH2 R172 mutations, respectively, in cell-free DNA (cfDNA) in the plasma. The combination of multicolor FCM of the blood, and tests for RHOA G17V and IDH2 R172 hot-spot mutations in plasma cfDNA provides a blood-based 'liquid biopsy' for the diagnosis of AITL.
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