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Tytuł:
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EBV Rta-induced IL-6 Promotes Migration of Bystander Tumor Cells Through IL-6R/JAK/STAT3 Pathway In Vitro .
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Autorzy:
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Tung KL; Department of Dental Technology, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan, R.O.C.
Wu YT; Department of Pathology, Golden Hospital, Pingtung, Taiwan, R.O.C.
Liu C; Department of Dental Technology, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan, R.O.C.; Department of Health and Beauty, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan, R.O.C.
Lin SC; Department of Dental Technology, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan, R.O.C.
Wu CH; Department of Dental Technology, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan, R.O.C.
Wu SY; Department of Pathology, Golden Hospital, Pingtung, Taiwan, R.O.C.
Chang Y; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Tainan, Taiwan, R.O.C.
Lan YY; Department of Physical Therapy, Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan, R.O.C. .
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Źródło:
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Anticancer research [Anticancer Res] 2020 Jun; Vol. 40 (6), pp. 3255-3264.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Attiki, Greece : International Institute of Anticancer Research
Original Publication: Athens, Greece : Potamitis Press
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MeSH Terms:
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Immediate-Early Proteins/*metabolism
Interleukin-6/*metabolism
Janus Kinases/*metabolism
Neoplasms/*metabolism
Receptors, Interleukin-6/*metabolism
STAT3 Transcription Factor/*metabolism
Trans-Activators/*metabolism
Breast Neoplasms/genetics ; Breast Neoplasms/metabolism ; Breast Neoplasms/pathology ; Breast Neoplasms/virology ; Cell Line, Tumor ; Cell Movement/physiology ; Humans ; Immediate-Early Proteins/genetics ; Interleukin-6/biosynthesis ; Interleukin-6/genetics ; MAP Kinase Signaling System ; MCF-7 Cells ; Nasopharyngeal Carcinoma/genetics ; Nasopharyngeal Carcinoma/metabolism ; Nasopharyngeal Carcinoma/pathology ; Nasopharyngeal Carcinoma/virology ; Nasopharyngeal Neoplasms/genetics ; Nasopharyngeal Neoplasms/metabolism ; Nasopharyngeal Neoplasms/pathology ; Nasopharyngeal Neoplasms/virology ; Neoplasms/genetics ; Neoplasms/pathology ; Neoplasms/virology ; Signal Transduction ; Stomach Neoplasms/genetics ; Stomach Neoplasms/metabolism ; Stomach Neoplasms/pathology ; Stomach Neoplasms/virology ; Trans-Activators/genetics ; Transcription Factor AP-1/metabolism ; Transfection ; Up-Regulation
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Contributed Indexing:
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Keywords: Epstein-Barr virus; Rta; cell migration; interleukin-6; nasopharyngeal carcinoma
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Substance Nomenclature:
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0 (BRLF1 protein, Human herpesvirus 4)
0 (IL6 protein, human)
0 (IL6R protein, human)
0 (Immediate-Early Proteins)
0 (Interleukin-6)
0 (Receptors, Interleukin-6)
0 (STAT3 Transcription Factor)
0 (STAT3 protein, human)
0 (Trans-Activators)
0 (Transcription Factor AP-1)
EC 2.7.10.2 (Janus Kinases)
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Entry Date(s):
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Date Created: 20200604 Date Completed: 20200716 Latest Revision: 20200716
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Update Code:
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20240104
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DOI:
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10.21873/anticanres.14307
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PMID:
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32487620
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Background/aim: Rta, a transactivator of Epstein-Barr virus, is associated with progression of nasopharyngel carcinoma (NPC); however, its mechanism of contribution to the pathogenesis of NPC remains unclear. Interleukin-6 (IL-6), a tumor promoter, is detected in NPC. This in vitro study examined whether and how Rta promotes NPC progression by up-regulating IL-6.
Materials and Methods: Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), quantitative real-time PCR, ELISA, immunoblotting assays, reporter gene assays, and transwell migration assays were performed.
Results: In NPC cells, Rta up-regulated IL-6 expression at the mRNA and protein levels, and the Rta's C-terminus was essential for promoter activation and expression of IL-6. The induction of IL-6 by Rta also required activation of extracellular signal-regulated kinase 1/2 and activator protein-1. Furthermore, IL-6 secreted from Rta-expressing NPC cells promoted migration of Rta-negative NPC cells by activating IL-6 receptor/Janus kinase/signal transducer and activator of transcription 3 pathway.
Conclusion: Rta contributes to progression of NPC cells through induction of IL-6 in vitro.
(Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)