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Tytuł:
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A 16-plex Y-SNP typing system based on allele-specific PCR for the genotyping of Chinese Y-chromosomal haplogroups.
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Autorzy:
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Zhou Z; Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
Zhou Y; Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
Yao Y; Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
Qian J; Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
Liu B; Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
Yang Q; Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
Shao C; Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
Li H; Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China; Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Research Institute of Criminal Science and Technology, Shanghai 200083, China.
Sun K; Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
Tang Q; Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
Xie J; Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China. Electronic address: .
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Źródło:
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Legal medicine (Tokyo, Japan) [Leg Med (Tokyo)] 2020 Sep; Vol. 46, pp. 101720. Date of Electronic Publication: 2020 May 30.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: <2003->: East Park, Co. Clare, Ireland : Elsevier Science Ireland Ltd.
Original Publication: Tokyo : Japanese Society of Legal Medicine, c1999-
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MeSH Terms:
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Alleles*
Genotyping Techniques*
Haplotypes*
Polymerase Chain Reaction*
Chromosomes, Human, Y/*genetics
Polymorphism, Single Nucleotide/*genetics
Asian People/genetics ; Female ; Humans ; Male
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Contributed Indexing:
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Keywords: Allele-specific PCR; Multiplex typing system; Y-chromosomal SNPs; Y-chromosomal haplogroup
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Entry Date(s):
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Date Created: 20200608 Date Completed: 20200907 Latest Revision: 20221207
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Update Code:
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20240104
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DOI:
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10.1016/j.legalmed.2020.101720
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PMID:
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32505804
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Y-chromosomal SNP (Y-SNP), with its stable inheritance and low mutation, can provide Supplementary information in forensic investigation. While commonly used Y-chromosomal STR haplotypes show their limitations, typing of Y-SNP would become a powerful complement. In this study, a 16-plex Y-SNP typing system based on allele-specific PCR (AS-PCR) was developed to discriminate four dominant Y-chromosomal haplogroups (C-M130, D-CTS3946, N-M231, and O-M175) and 12 predominant sub-haplogroups of O-M175 (O1a-M119, O1a1a1a-CTS3265, O1b-M268, O1b1a2-Page59, O2-M122, O2a1-L127.1, O2a1b-F240, O2a1b1a1-CTS5820, O2a2-P201, O2a2b1a1-M177, O2a2b1a1a1a-Y17728, O2a2b1a2-F114). A series of experimental validation studies including sensitivity, species specificity, male-female mixture and inhibition were performed. The discrimination of the typing system was preliminarily proved with a haplogroup diversity of 0.9239. Altogether, the Y-SNP typing system based on AS-PCR should be capable of distinguishing China's dominant Y-chromosomal haplogroups in a rapid and reliable manner, thus can be employed as a useful complement in forensic casework.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
