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Tytuł:
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Induction of ZCCHC6 expression in peripheral blood mononuclear cells by HNSCC secretions.
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Autorzy:
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Arayataweegool A; Center of Excellence in Molecular Genetics of Cancer and Human Diseases, Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Srisuttee R; Faculty of Medicine, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
Bin-Alee F; Program of Medical Science, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Mahattanasakul P; Department of Otolaryngology, Head and Neck Surgery, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand; Department of Otolaryngology, Head and Neck Surgery, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok 10330, Thailand.
Tangjaturonrasme N; Department of Otolaryngology, Head and Neck Surgery, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Kerekhanjanarong V; Department of Otolaryngology, Head and Neck Surgery, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Mutirangura A; Center of Excellence in Molecular Genetics of Cancer and Human Diseases, Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Kitkumthorn N; Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand. Electronic address: .
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Źródło:
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Gene [Gene] 2020 Sep 05; Vol. 754, pp. 144880. Date of Electronic Publication: 2020 Jun 09.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Amsterdam, Elsevier/North-Holland, 1976-
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MeSH Terms:
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Transcriptome*
Biomarkers, Tumor/*blood
Head and Neck Neoplasms/*blood
Leukocytes, Mononuclear/*metabolism
RNA Nucleotidyltransferases/*blood
Squamous Cell Carcinoma of Head and Neck/*blood
Adult ; Aged ; Aged, 80 and over ; Case-Control Studies ; Female ; Follow-Up Studies ; Head and Neck Neoplasms/genetics ; Head and Neck Neoplasms/pathology ; Humans ; Leukocytes, Mononuclear/pathology ; Male ; Middle Aged ; Prognosis ; Squamous Cell Carcinoma of Head and Neck/genetics ; Squamous Cell Carcinoma of Head and Neck/pathology
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Contributed Indexing:
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Keywords: Expression array; Head and neck squamous cell carcinoma (HNSCC); Peripheral blood mononuclear cells (PBMCs); RNA sequencing; ZCCHC6 expression
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Substance Nomenclature:
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0 (Biomarkers, Tumor)
EC 2.7.7.- (RNA Nucleotidyltransferases)
EC 2.7.7.52 (TUT7 protein, human)
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Entry Date(s):
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Date Created: 20200612 Date Completed: 20200805 Latest Revision: 20200805
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Update Code:
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20240105
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DOI:
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10.1016/j.gene.2020.144880
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PMID:
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32526260
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Cancer secretion can change the properties of adjacent cells, including peripheral blood mononuclear cells (PBMCs). We investigated whether such secretion influences messenger RNA expression in PBMCs of patients with head and neck squamous cell carcinoma (HNSCC). In the present study, co-culture model of normal PBMCs and HNSCC cell lines were established. The PBMCs were subsequently subjected to RNA sequencing for transcriptome analysis. Furthermore, expression data from the Gene Expression Omnibus repository, platform GPL4133, series GSE39400, were gathered to analyze, afterward identify zinc finger CysCysHisCys (CCHC)-type domain-containing protein 6 (ZCCHC6) as the main gene involved in HNSCC. This gene was then validated by a quantitative real-time polymerase chain reaction. The results showed that ZCCHC6 was expressed at significantly higher levels in the patients with HNSCC than in the healthy controls, and the sensitivity and specificity of these findings for diagnostic purposes were 100.00% and 70.83%, respectively. In summary, our findings demonstrated that the secretion of HNSCC cells could cause the alterations in messenger RNA expression by PBMCs. The ZCCHC6 expression level may apply in HNSCC screening.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
