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Tytuł pozycji:

One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses.

Tytuł :
One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses.
Autorzy :
Wang R; MOE Joint International Research Laboratory of Animal Health and Food Safety, Engineering Laboratory of Animal Immunity of Jiangsu Province, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Zhang W; MOE Joint International Research Laboratory of Animal Health and Food Safety, Engineering Laboratory of Animal Immunity of Jiangsu Province, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Ye R; MOE Joint International Research Laboratory of Animal Health and Food Safety, Engineering Laboratory of Animal Immunity of Jiangsu Province, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Pan Z; MOE Joint International Research Laboratory of Animal Health and Food Safety, Engineering Laboratory of Animal Immunity of Jiangsu Province, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Li G; MOE Joint International Research Laboratory of Animal Health and Food Safety, Engineering Laboratory of Animal Immunity of Jiangsu Province, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Su S; MOE Joint International Research Laboratory of Animal Health and Food Safety, Engineering Laboratory of Animal Immunity of Jiangsu Province, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China. Electronic address: .
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Źródło :
Molecular and cellular probes [Mol Cell Probes] 2020 Oct; Vol. 53, pp. 101618. Date of Electronic Publication: 2020 Jun 10.
Typ publikacji :
Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
Język :
English
Imprint Name(s) :
Original Publication: London ; New York : Academic Press, c1987-
MeSH Terms :
DNA Probes*
Diarrhea/*veterinary
Dog Diseases/*virology
Real-Time Polymerase Chain Reaction/*veterinary
Animals ; Avastrovirus ; Coronavirus, Canine ; Diarrhea/diagnosis ; Diarrhea/virology ; Dog Diseases/diagnosis ; Dogs ; Kobuvirus ; Parvovirus, Canine ; Real-Time Polymerase Chain Reaction/methods ; Reproducibility of Results ; Sensitivity and Specificity
References :
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Contributed Indexing :
Keywords: Canine diarrhea*; Multiplex real-time PCR*; TaqMan probe*
Substance Nomenclature :
0 (DNA Probes)
Entry Date(s) :
Date Created: 20200614 Date Completed: 20200929 Latest Revision: 20200929
Update Code :
20201218
PubMed Central ID :
PMC7286240
DOI :
10.1016/j.mcp.2020.101618
PMID :
32534013
Czasopismo naukowe
Viral canine diarrhea has high morbidity and mortality and is prevalent worldwide, resulting in severe economic and spiritual losses to pet owners. However, diarrhea pathogens have similar clinical symptoms and are difficult to diagnose clinically. Thus, fast and accurate diagnostic methods are of great significance for prevention and accurate treatment. In this study, we developed a one-step multiplex TaqMan probe-based real-time PCR for the differential diagnosis of four viruses causing canine diarrhea including, CPV (Canine Parvovirus), CCoV (Canine Coronavirus), CAstV (Canine Astrovirus), and CaKoV (Canine Kobuviruses). The limit of detection was up to 10 2 copies/μL and performed well with high sensitivity and specificity. This assay was optimized and used to identify possible antagonistic relationships between viruses. From this, artificial pre-experiments were performed for mixed infections, and a total of 82 canine diarrhea field samples were collected from different animal hospitals in Zhejiang, China to assess the method. The virus prevalence was significantly higher than what previously reported based on RT-PCR (Reverse Transcription-Polymerase Chain Reaction). Taken together, these results suggest that the method can be used as a preferred tool for monitoring laboratory epidemics, timely prevention, and effective monitoring of disease progression.
(Copyright © 2020. Published by Elsevier Ltd.)

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