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Tytuł:
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Recipient interleukin 6 gene polymorphism and expression predict HCV recurrence post liver transplantation.
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Autorzy:
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Dawood RM; Department of Microbial Biotechnology, Genetic Engineering Division, National Research Centre, 33 EL Bohouth Street Dokki, Giza 12622, Egypt. Electronic address: .
Salum GM; Department of Microbial Biotechnology, Genetic Engineering Division, National Research Centre, 33 EL Bohouth Street Dokki, Giza 12622, Egypt.
Abd El-Meguid M; Department of Microbial Biotechnology, Genetic Engineering Division, National Research Centre, 33 EL Bohouth Street Dokki, Giza 12622, Egypt.
Shemis M; Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Egypt.
Abdel Aziz AO; Department of Endemic Medicine and Hepatogastroenterology, Faculty of Medicine, Cairo University, Egypt.
El Awady MK; Department of Microbial Biotechnology, Genetic Engineering Division, National Research Centre, 33 EL Bohouth Street Dokki, Giza 12622, Egypt.
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Źródło:
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Gene [Gene] 2020 Sep 05; Vol. 754, pp. 144887. Date of Electronic Publication: 2020 Jun 10.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Amsterdam, Elsevier/North-Holland, 1976-
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MeSH Terms:
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Polymorphism, Single Nucleotide*
Hepacivirus/*physiology
Hepatitis C/*blood
Interleukin-6/*blood
Interleukin-6/*genetics
Leukocytes, Mononuclear/*metabolism
Liver Transplantation/*adverse effects
Female ; Hepatitis C/surgery ; Hepatitis C/virology ; Humans ; Leukocytes, Mononuclear/virology ; Male ; Middle Aged ; Recurrence ; Transplant Recipients
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Contributed Indexing:
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Keywords: HCV; Hepatocellular carcinoma; Interleukin 6; Living donor liver transplantation; Recurrence
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Substance Nomenclature:
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0 (IL6 protein, human)
0 (Interleukin-6)
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Entry Date(s):
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Date Created: 20200614 Date Completed: 20200805 Latest Revision: 20200805
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Update Code:
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20240105
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DOI:
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10.1016/j.gene.2020.144887
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PMID:
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32534059
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Background: Liver transplantation (LTX)is a lifesaving- effective protocol for patients suffering end stage liver disease (ESLD) and its complications post HCV infection. Recurrence of disease is a frequent clinical complication that is observed in patients undergoing LTX. Cytokines play a central role in the immunological events occurring after the surgery.
Methods: Using Allelic Discrimination PCR, the allelic variation G174C of IL-6 gene was investigated. The abundance of IL6- mRNA and plasma IL6 cytokine levels were evaluated by using qRT-PCR in peripheral blood mononuclear cells (PBMCs) and enzyme-linked immunosorbent assay (ELISA) respectively in 76 liver transplant recipients recruited from Al Sahel teaching hospital, Ministry of Health and Population Cairo Egypt within the period between June 2015 and October 2017.
Results: The frequencies of IL-6 GG genotype and the G allele were significantly detected more in LTX recipients who experienced HCV recurrence versus those who did not suffer recurrence when compared to healthy controls (P = 0.001) and (P = 0.006), respectively. On the contrary, levels of IL-6 related transcripts in PBMC's of recurrent patients were indifferent from non-recurrent patients and healthy controls (P ≥ 0.124). Interestingly, the circulating IL-6 protein in plasma was significantly elevated in recurrent as compared to the non-recurrent recipients (P = 0.002).
Conclusion: HCV recurrence post liver transplantation occur more frequently in patients with -174 G/G IL-6 genotype and elevated plasma IL-6 levels.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
