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Tytuł:
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Synergistic bactericidal effect and mechanism of X-ray irradiation and citric acid combination against food-borne pathogens on spinach leaves.
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Autorzy:
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Jeon MJ; Department of Food Science and Biotechnology, Global K-Food Research Center, Hankyong National University, Anseong-si, 17579, South Korea.
Ha JW; Department of Food Science and Biotechnology, Global K-Food Research Center, Hankyong National University, Anseong-si, 17579, South Korea. Electronic address: .
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Źródło:
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Food microbiology [Food Microbiol] 2020 Oct; Vol. 91, pp. 103543. Date of Electronic Publication: 2020 May 06.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: London : Elsevier
Original Publication: London ; Orlando : Academic Press, c1984-
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MeSH Terms:
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Citric Acid/*pharmacology
Disinfectants/*pharmacology
Food Irradiation/*methods
Spinacia oleracea/*microbiology
Cell Membrane/drug effects ; Cell Membrane/radiation effects ; Colony Count, Microbial ; Escherichia coli O157/drug effects ; Escherichia coli O157/growth & development ; Escherichia coli O157/metabolism ; Escherichia coli O157/radiation effects ; Food Microbiology ; Listeria monocytogenes/drug effects ; Listeria monocytogenes/growth & development ; Listeria monocytogenes/metabolism ; Listeria monocytogenes/radiation effects ; Plant Leaves/microbiology ; Reactive Oxygen Species/metabolism ; X-Rays
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Contributed Indexing:
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Keywords: Citric acid; Foodborne pathogens; Inactivation; Mechanism; Spinach; X-ray irradiation
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Substance Nomenclature:
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0 (Disinfectants)
0 (Reactive Oxygen Species)
2968PHW8QP (Citric Acid)
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Entry Date(s):
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Date Created: 20200617 Date Completed: 20210121 Latest Revision: 20210121
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Update Code:
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20240105
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DOI:
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10.1016/j.fm.2020.103543
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PMID:
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32539944
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In this study, we investigated the antimicrobial activity of the X-ray irradiation and citric acid (CA) combination against Escherichia coli O157:H7 and Listeria monocytogenes on the surface of spinach leaves and elucidated the mechanisms underlying their synergistic interaction. Upon treatment with 0.3 kGy X-ray irradiation and 1% CA combination, the cell counts of E. coli O157:H7 and L. monocytogenes reduced by 4.23 and 3.69 log CFU/mL on spinach leaves, respectively. The synergistic reduction in the cell counts of E. coli O157:H7 and L. monocytogenes by the combination treatment was 0.95 and 1.14 log units, respectively. The X-ray and CA combination exerts its antimicrobial effect by damaging the bacterial cell membrane and enhancing the generation of intracellular reactive oxygen species in the pathogens. The enhanced bactericidal effect of the combination treatment may not be due to the loss of intracellular enzyme activity. We also evaluated the effect of the combination treatment on the quality attributes of spinach leaves. The combination treatment did not result in adverse changes in color and texture of spinach leaves. These results demonstrate the potential of citric acid and X-ray irradiation combination for decontaminating foodborne pathogens on fresh produce.
Competing Interests: Declaration of competing interest The authors (Min-Jin Jeon, Jae-Won Ha) declare that they have no competing interest.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
