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Tytuł:
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Application of qPCR for multicopper oxidase gene (MCO) in biogenic amines degradation by Lactobacillus casei.
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Autorzy:
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Pištěková H; Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 76001, Zlín, Czech Republic.
Jančová P; Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 76001, Zlín, Czech Republic. Electronic address: .
Berčíková L; Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 76001, Zlín, Czech Republic.
Buňka F; Department of Food Technology, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 76001, Zlín, Czech Republic.
Sokolová I; Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 76001, Zlín, Czech Republic.
Šopík T; Department of Food Technology, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 76001, Zlín, Czech Republic.
Maršálková K; Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 76001, Zlín, Czech Republic.
Amaral OMRP; Department of Technologies and Applied Sciences, Escola Superior Agária, Instituto Politécnico de Beja, Rua Pedro Soares S/N, Apartado 6155, 7800-295, Beja, Portugal.
Buňková L; Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 76001, Zlín, Czech Republic.
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Źródło:
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Food microbiology [Food Microbiol] 2020 Oct; Vol. 91, pp. 103550. Date of Electronic Publication: 2020 May 23.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: London : Elsevier
Original Publication: London ; Orlando : Academic Press, c1984-
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MeSH Terms:
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Bacterial Proteins/*genetics
Biogenic Amines/*metabolism
Lacticaseibacillus casei/*metabolism
Oxidoreductases/*genetics
Animals ; Ascorbic Acid/analysis ; Ascorbic Acid/metabolism ; Bacterial Proteins/metabolism ; Biogenic Amines/analysis ; Chromatography, High Pressure Liquid ; Culture Media/chemistry ; Cysteine/analysis ; Cysteine/metabolism ; Gene Expression Regulation, Bacterial ; Glyceraldehyde-3-Phosphate Dehydrogenases/genetics ; Lactobacillus/enzymology ; Lactobacillus/genetics ; Lactobacillus/growth & development ; Lactobacillus/metabolism ; Lacticaseibacillus casei/enzymology ; Lacticaseibacillus casei/genetics ; Lacticaseibacillus casei/growth & development ; Milk/chemistry ; Oxidoreductases/metabolism ; Real-Time Polymerase Chain Reaction
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Contributed Indexing:
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Keywords: Biogenic amines degradation; Histamine; Lactobacillus casei; Primers; qPCR
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Substance Nomenclature:
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0 (Bacterial Proteins)
0 (Biogenic Amines)
0 (Culture Media)
EC 1.- (Oxidoreductases)
EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
K848JZ4886 (Cysteine)
PQ6CK8PD0R (Ascorbic Acid)
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Entry Date(s):
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Date Created: 20200617 Date Completed: 20210121 Latest Revision: 20221207
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Update Code:
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20240105
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DOI:
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10.1016/j.fm.2020.103550
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PMID:
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32539976
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Degradation of undesirable biogenic amines (BAs) in foodstuffs by microorganisms is considered one of the most effective ways of eliminating their toxicity. In this study, we designed two sets of primers for the detection and quantification of the multicopper oxidase gene (MCO), which encodes an enzyme involved in BAs degradation, and endogenous (glyceraldehyde-3-phosphate dehydrogenase) gene (GAPDH) in Lactobacillus casei group by real-time PCR (qPCR). We tested 15 Lactobacillus strains in the screening assays (thus, MCO gene possessing assay (PCR) and monitoring of BAs degradation by HPLC-UV), in which Lactobacillus casei CCDM 198 exhibited the best degradation abilities. For this strain, we monitored the expression of the target gene (MCO) in time (qPCR), the effect of redox treatments (cysteine, ascorbic acid) on the expression of the gene, and the ability to degrade BAs not only in a modified MRS medium (MRS/2) but also in a real food sample (milk). Moreover, decarboxylase activity (ability to form BAs) of this strain was excluded. According to the results, CCDM 198 significantly (P < 0.05) reduced BAs (putrescine, histamine, tyramine, cadaverine), up to 25% decline in 48 h. The highest level of relative expression of MCO (5.21 ± 0.14) was achieved in MRS/2 media with cysteine.
Competing Interests: Declaration of competing interest None.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
