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Tytuł pozycji:

[Determination of UV-327 and UV-328 in mouse plasma by high performance liquid chromatography].

Tytuł :
[Determination of UV-327 and UV-328 in mouse plasma by high performance liquid chromatography].
Autorzy :
Zhu MQ; Department of Occupational and Environmental Health Science, Peking University School of Public Health, Beijing 100191, China.
Cui R; Department of Occupational and Environmental Health Science, Peking University School of Public Health, Beijing 100191, China.
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Źródło :
Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences [Beijing Da Xue Xue Bao Yi Xue Ban] 2020 Jun 18; Vol. 52 (3), pp. 591-596.
Typ publikacji :
Journal Article
Język :
Chinese
Imprint Name(s) :
Original Publication: Beijing : Beijing da xue, 2001-
MeSH Terms :
Chromatography, High Pressure Liquid*
Animals ; Calibration ; Mice ; Plasma ; Reproducibility of Results ; Ultraviolet Rays
References :
Drug Chem Toxicol. 2008;31(3):383-98. (PMID: 18622872)
J Chromatogr A. 2015 Mar 6;1384:97-106. (PMID: 25637008)
J Chromatogr A. 2016 Aug 26;1461:51-8. (PMID: 27425756)
Environ Sci Technol. 2006 Dec 1;40(23):7157-66. (PMID: 17180962)
Drug Chem Toxicol. 2007;30(4):327-41. (PMID: 17934922)
Sci Total Environ. 2019 Jan 10;647:182-190. (PMID: 30081360)
Drug Chem Toxicol. 2008;31(1):81-96. (PMID: 18161509)
Environ Sci Technol. 2009 Sep 15;43(18):6920-6. (PMID: 19806721)
Environ Int. 2015 Jul;80:1-7. (PMID: 25827264)
Environ Res. 2015 Jul;140:466-73. (PMID: 25988989)
J Environ Monit. 2010 Nov;12(11):2088-92. (PMID: 20959922)
Chemosphere. 2011 Oct;85(5):751-8. (PMID: 21741069)
Contributed Indexing :
Keywords: High performance liquid chromatography; Mouse; Plasma; UV-327; UV-328
Entry Date(s) :
Date Created: 20200617 Date Completed: 20200806 Latest Revision: 20200917
Update Code :
20201023
PubMed Central ID :
PMC7433428
DOI :
10.19723/j.issn.1671-167X.2020.03.030
PMID :
32541998
Czasopismo naukowe
Objective: To establish a high performance liquid chromatography (HPLC) method for the determination of ultraviolet (UV) absorbers UV-327 and UV-328 in mouse plasma.
Methods: N-hexane-acetone (volume ratio 1 ∶1) was added to a mouse plasma sample as the extraction solvent for vortex extraction, and the supernatant was dried at 50 ℃ with nitrogen. Thereafter the residue was redissolved with methanol, centrifuged and filtered. The separation was performed on a Waters SymmetryC 18 column (250 mm×4.6 mm, 5 μm), and the concentrations of UV-327 and UV-328 in the mouse plasma were determined by HPLC with an UV detector. The elution was isocratic at a flow rate of 1.0 mL/min with a mobile phase composed of 100% methanol, and the UV detection wavelength was 340 nm. The retention time was used for qualitative analysis, and the internal standard method was used for quantitative analysis using UV-320 as the internal standard.
Results: The calibration curves of UV-327 and UV-328 were linear with correlation coefficients of 0.999 7 over the concentration range of 0.05 to 10.0 mg/L. The limit of detection was 0.01 mg/L, and the limit of quantitation was 0.03 mg/L. The average recoveries at low, medium and high three concentrations (0.50, 1.00, 2.00 mg/L) in the mouse plasma were 91.7%-101.0% for UV-327, and 97.5%-103.9% for UV-328. The intra-day precisions ( n =6) of UV-327 were 2.9%-6.6%, and 2.7%-7.4% for UV-328. The inter-day precisions ( n =3) of UV-327 were 6.0%-9.3%, and 6.6%-8.6% for UV-328. The extraction recoveries of UV-327 were 98.8%-103.8%, and 99.8%-100.9% for UV-328. The measured relative deviations of UV-327 in the mouse plasma samples placed at room temperature for 6 hours and -40 ℃ for 15 days were 0.9%-3.5% and 7.4%-15.0%, and the measured relative deviations of UV-328 were 2.0%-4.3% and 2.1%-13.8%, respectively. The mouse plasma samples could be stored at room temperature for 6 hours at least and -40 ℃ for 15 days at three spiked concentration levels.
Conclusion: The method was simple and fast with high accuracy, precision and sensitivity, and could be applied to the determination of UV-327 and UV-328 in mouse plasma.

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