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Tytuł:
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Haploid pluripotent stem cells: twofold benefits with half the effort in genetic screening and reproduction.
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Autorzy:
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Li Y; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Li W; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Zhou Q; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: .
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Źródło:
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Current opinion in genetics & development [Curr Opin Genet Dev] 2020 Oct; Vol. 64, pp. 6-12. Date of Electronic Publication: 2020 Jun 18.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't; Review
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Język:
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English
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Imprint Name(s):
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Publication: London : Elsevier
Original Publication: London, UK : Current Biology, c1991-
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MeSH Terms:
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Genome*
Genomic Imprinting*
Haploidy*
Reproduction*
Genetic Testing/*methods
Pluripotent Stem Cells/*cytology
Animals ; Cell Differentiation ; Gene Editing ; Humans ; Pluripotent Stem Cells/metabolism
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Entry Date(s):
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Date Created: 20200622 Date Completed: 20210702 Latest Revision: 20210702
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Update Code:
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20240105
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DOI:
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10.1016/j.gde.2020.05.013
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PMID:
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32563751
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Haploid pluripotent stem cells, which are capable of self-renewal and differentiation into other cell types with only one set of chromosomes, have been established in several species from haploid embryos. Compared with diploid embryonic stem cells (ESCs), haploid embryonic stem cells (haESCs) are smaller in size, have a prolonged metaphase, and undergo self-doubling during culture. The monoallelic expression of haESCs provides great convenience for recessive inheritance research. Genetically modified haESCs also provide benefits in replacement of the gamete genomes, which not only facilitates the study of the function of imprinted genes but also potentially removes barriers to same-sex reproduction. In this review, we focus on strategies for obtaining haESCs and their potential applications in genetic screening, genomic imprinting, and unisexual reproduction.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)