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Tytuł pozycji:

Next-generation sequencing of residual cytologic fixative preserved DNA from pancreatic lesions: A pilot study.

Tytuł :
Next-generation sequencing of residual cytologic fixative preserved DNA from pancreatic lesions: A pilot study.
Autorzy :
Fulmer CG; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York.; Robert J. Tomsich Pathology and Laboratory Medicine Institute, The Cleveland Clinic, Cleveland, Ohio.
Park K; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York.
Dilcher T; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York.
Ho M; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York.
Mirabelli S; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York.
Alperstein S; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York.
Hissong EM; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York.
Pittman M; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York.
Siddiqui M; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York.
Heymann JJ; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York.
Yantiss RK; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York.
Borczuk AC; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York.
Fernandes H; Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, New York.
Sigel C; Department of Pathology, The Memorial Sloan Kettering Cancer Center, New York, New York.
Song W; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York.
Mosquera JM; Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York.; Englander Institute for Precision Medicine, Weill Cornell Medicine, New York, New York.
Rao R; The Leopold G. Koss Division of Cytology, The Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, New York.
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Źródło :
Cancer cytopathology [Cancer Cytopathol] 2020 Nov; Vol. 128 (11), pp. 840-851. Date of Electronic Publication: 2020 Jun 29.
Typ publikacji :
Journal Article
Język :
English
Imprint Name(s) :
Original Publication: Hoboken, NJ : Wiley-Blackwell
MeSH Terms :
Mutation*
Biomarkers, Tumor/*genetics
Carcinoma, Pancreatic Ductal/*diagnosis
High-Throughput Nucleotide Sequencing/*methods
Neuroendocrine Tumors/*diagnosis
Pancreatic Cyst/*diagnosis
Pancreatic Neoplasms/*diagnosis
Adult ; Aged ; Aged, 80 and over ; Carcinoma, Pancreatic Ductal/genetics ; Carcinoma, Pancreatic Ductal/surgery ; Cytological Techniques ; Endoscopic Ultrasound-Guided Fine Needle Aspiration ; Female ; Humans ; Male ; Middle Aged ; Neuroendocrine Tumors/genetics ; Neuroendocrine Tumors/surgery ; Pancreatic Cyst/genetics ; Pancreatic Cyst/surgery ; Pancreatic Neoplasms/genetics ; Pancreatic Neoplasms/surgery ; Pilot Projects ; Prognosis ; Specimen Handling ; Young Adult
References :
Arnold M, Rutherford MJ, Bardot A, et al. Progress in cancer survival, mortality, and incidence in seven high-income countries 1995-2014 (ICBP SURVMARK-2): a population-based study. Lancet Oncol. 2019;20:1493-1505.
Thierry C, Desseigne F, Ychou M, et al. FOLFIRINOX versus gemcitabine for metastatic pancreatic cancer. N Engl J Med. 2011;364:1817-1825.
Allen PJ, Kuk D, Fernandez-Del Castillo C, et al. Multi-institutional validation study of the American Joint Commission on Cancer (8th edition) changes for T and N staging in patients with pancreatic adenocarcinoma. Ann Surg. 2017;265:185-191.
Saad AM, Turk T, Al-Husseini MJ, Abdel-Rahman O. Trends in pancreatic adenocarcinoma incidence and mortality in the United States in the last four decades; a SEER-based study. BMC Cancer. 2018;18:688.
Kayahara M, Nagakawa T, Ueno K, Ohta T, Takeda T, Miyazaki I. An evaluation of radical resection for pancreatic cancer based on the mode of recurrence as determined by autopsy and diagnostic imaging. Cancer. 1993;72:2118-2123.
Conlon KC, Klimstra DS, Brennan MF. Long-term survival after curative resection for pancreatic ductal adenocarcinoma. Clinicopathologic analysis of 5-year survivors. Ann Surg. 1996;223:273-279.
Wang W, Shpaner A, Krishna SG, et al. Use of EUS-FNA in diagnosing pancreatic neoplasm without a definitive mass on CT. Gastrointest Endosc. 2013;78:73-80.
Puli SR, Bechtold ML, Buxbaum JL, Eloubeidi MA. How good is endoscopic ultrasound-guided fine-needle aspiration in diagnosing the correct etiology for a solid pancreatic mass?: A meta-analysis and systematic review. Pancreas. 2013;42:20-26.
Woolf KMW, Liang H, Sletten ZJ, Russell DK, Bonfiglio TA, Zhou Z. False-negative rate of endoscopic ultrasound-guided fine-needle aspiration for pancreatic solid and cystic lesions with matched surgical resections as the gold standard: one institution's experience. Cancer Cytopathol. 2013;121:449-458.
Collins BT, Murad FM, Wang JF, Bernadt CT. Rapid on-site evaluation for endoscopic ultrasound-guided fine-needle biopsy of the pancreas decreases the incidence of repeat biopsy procedures. Cancer Cytopathol. 2013;121:518-524.
Oh SH, Lee JK, Lee KT, Lee KH, Woo YS, Noh DH. The combination of cyst fluid carcinoembryonic antigen, cytology and viscosity increases the diagnostic accuracy of mucinous pancreatic cysts. Gut Liver. 2017;11:283-289.
Pitman MB, Centeno BA, Genevay M, Fonseca R, Mino-Kenudson M. Grading epithelial atypia in endoscopic ultrasound-guided fine-needle aspiration of intraductal papillary mucinous neoplasms: an international interobserver concordance study. Cancer Cytopathol. 2013;121:729-736.
Pitman MB, Centeno BA, Daglilar ES, Brugge WR, Mino-Kenudson M. Cytological criteria of high-grade epithelial atypia in the cyst fluid of pancreatic intraductal papillary mucinous neoplasms. Cancer Cytopathol. 2014;122:40-47.
Ogura T, Yamao K, Sawaki A, et al. Clinical impact of K-ras mutation analysis in EUS-guided FNA specimens from pancreatic masses. Gastrointest Endosc. 2012;75:769-774.
Khalid A, Dewitt J, Ohori NP, et al. EUS-FNA mutational analysis in differentiating autoimmune pancreatitis and pancreatic cancer. Pancreatology. 2011;11:482-486.
Khalid A, Zahid M, Finkelstein SD, et al. Pancreatic cyst fluid DNA analysis in evaluating pancreatic cysts: a report of the PANDA study. Gastrointest Endosc. 2009;69:1095-1102.
Nikiforova MN, Khalid A, Fasanella KE, et al. Integration of KRAS testing in the diagnosis of pancreatic cystic lesions: a clinical experience of 618 pancreatic cysts. Mod Pathol. 2013;26:1478-1487.
Wei S, Lieberman D, Morrissette JJD, et al. Using “residual” FNA rinse and body fluid specimens for next-generation sequencing: an institutional experience. Cancer Cytopathol. 2016;124:324-329.
Fuller MY, Mody D, Hull A, Pepper K, Hendrickson H, Olsen R. Next-generation sequencing identifies gene mutations that are predictive of malignancy in residual needle rinses collected from fine-needle aspirations of thyroid nodules. Arch Pathol Lab Med. 2018;142:178-183.
Cheng DT, Mitchell TN, Zehir A, et al. Memorial Sloan Kettering-Integrated Mutation Profiling of Actionable Cancer Targets (MSK-IMPACT): a hybridization capture-based next-generation sequencing clinical assay for solid tumor molecular oncology. J Mol Diagn. 2015;17:251-264.
Rosenbaum MW, Jones M, Dudley JC, Le LP, Iafrate IJ, Pitman MB. Next-generation sequencing adds value to the preoperative diagnosis of pancreatic cysts. Cancer Cytopathol. 2017;125:41-47.
Jones M, Zheng Z, Wang J, et al. Impact of next-generation sequencing on the clinical diagnosis of pancreatic cysts. Gastrointest Endosc. 2016;83:140-148.
Springer S, Wang Y, Dal Molin M, et al. A combination of molecular markers and clinical features improve the classification of pancreatic cysts. Gastroenterology. 2015;149:1501-1510.
Reynolds JP, Zhou Y, Jakubowski MA, et al. Next-generation sequencing of liquid-based cytology non-small cell lung cancer samples. Cancer Cytopathol. 2017;125:178-187.
Dudley JC, Zheng Z, McDonald T, et al. Next-generation sequencing and fluorescence in situ hybridization have comparable performance characteristics in the analysis of pancreaticobiliary brushings for malignancy. J Mol Diagn. 2016;18:124-130.
Roy-Chowdhuri S, Mehrotra M, Bolivar AM, et al. Salvaging the supernatant: next generation cytopathology for solid tumor mutation profiling. Mod Pathol. 2018;31:1036-1045.
Zehir A, Benayed R, Shar RH, et al. Mutational landscape of metastatic cancer revealed from prospective clinical sequencing of 10,000 patients. Nat Med. 2017;23:703-713. Published correction appears in Nat Med. 2017;23:1004.
Suenaga M, Yu J, Shindo K, et al. Pancreatic juice mutation concentrations can help predict the grade of dysplasia in patients undergoing pancreatic surveillance. Clin Cancer Res. 2018;24:2963-2974.
Goyal A, Abdul-Karim FW, Yang B, Patel JB, Brainard JA. Interobserver agreement in the cytologic grading of atypia in neoplastic pancreatic mucinous cysts with the 2-tiered approach. Cancer Cytopathol. 2016;124:909-916.
AACR Project GENIE Consortium. AACR Project GENIE: Powering Precision Medicine through an International Consortium. Cancer Discov. 2017;7:818-831.
Schlitter AM, Segler A, Steiger K, et al. Molecular, morphological and survival analysis of 177 resected pancreatic ductal adenocarcinomas (PDACs): identification of prognostic subtypes. Sci Rep. 2017;7:41064.
Piskorz AM, Ennis D, Macintyre G, et al. Methanol-based fixation is superior to buffered formalin for next-generation sequencing of DNA from clinical cancer samples. Ann Oncol. 2016;27:532-539.
Ludgate JL, Wright J, Stockwell PA, Morison IM, Eccles MR, Chatterjee A. A streamlined method for analysing genome-wide DNA methylation patterns from low amounts of FFPE DNA. BMC Med Genomics. 2017;10:54.
Mishra NK, Guda C. Genome-wide DNA methylation analysis reveals molecular subtypes of pancreatic cancer. Oncotarget. 2017;8:28990-29012.
Nones K, Waddell N, Song S, et al. Genome-wide DNA methylation patterns in pancreatic ductal adenocarcinoma reveal epigenetic deregulation of SLIT-ROBO, ITGA2 and MET signaling. Int J Cancer. 2014;135:1110-1118.
Negri G, Rigo B, Vittadello F, Egarter-Vigl E, Mian C. Human papillomavirus typing with hybrid capture II on archived liquid-based cytologic specimens: is HPV typing always reproducible? Am J Clin Pathol. 2004;122:90-93.
Castle PE, Solomon D, Hildesheim A, et al. Stability of archived liquid-based cervical cytologic specimens. Cancer. 2003;99:89-96.
Agreda PM, Beitman GH, Gutierrez EC, et al. Long-term stability of human genomic and human papillomavirus DNA stored in BD SurePath and Hologic PreservCyt liquid-based cytology media. J Clin Microbiol. 2013;51:2702-2706.
Jiao Y, Shi C, Edil BH, et al. DAXX/ATRX, MEN1, and mTOR pathway genes are frequently altered in pancreatic neuroendocrine tumors. Science. 2011;331:1199-1203.
Chai SM, Herba K, Kumarasinghe MP, et al. Optimizing the multimodal approach to pancreatic cyst fluid diagnosis. Cancer Cytopathol. 2013;121:86-100.
Contributed Indexing :
Keywords: DNA*; endoscopic ultrasound-guided fine needle aspiration*; pancreatic carcinoma*; pancreatic ductal carcinoma*; pancreatic neoplasms*; sequence analysis*
Substance Nomenclature :
0 (Biomarkers, Tumor)
Entry Date(s) :
Date Created: 20200630 Date Completed: 20210223 Latest Revision: 20210223
Update Code :
20210309
DOI :
10.1002/cncy.22315
PMID :
32598087
Czasopismo naukowe
Background: Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) is a sensitive and specific tool in the risk stratification of pancreatic lesions, including cysts. The sensitivity and specificity of EUS-FNA has been shown to improve when cytology is combined with next-generation sequencing (NGS). Ideally, fresh cyst fluid is used for NGS. In this pilot study, we explore the possibility of sequencing DNA derived from residual alcohol-fixed pancreatic aspirates.
Methods: Residual cytologic fixatives (n = 42) from 39 patients who underwent EUS-FNA for pancreatic lesions were collected along with demographics, imaging, and laboratory studies. Samples were designated as nonneoplastic/nonmucinous benign (NB), mucinous cyst (MC), pancreatic ductal adenocarcinoma (PDAC), or well-differentiated neuroendocrine tumor (NET) on the basis of cytopathologic evaluation and sequenced on the Oncomine platform (ThermoFisher Scientific, Waltham, Massachusetts).
Results: Ten of 14 (71.4%) MCs exhibited clinically significant variants, including KRAS, GNAS, and TP53. Ten of 15 (66.7%) PDACs had KRAS alterations, and 9 of 15 (60%) showed variants in TP53. No variants were detected in any NETs. Only 1 of 9 (11.1%) NB aspirates showed variants in KRAS and MAP2K. Sequencing of formalin-fixed, paraffin-embedded tissue revealed variants identical to those detected in fixative-derived DNA in 4 of 5 cases (80%).
Conclusion: Residual DNA from alcohol-fixed aspirates are an underutilized source for NGS. Sequencing residual fixative-derived DNA has the potential to be integrated into the workup of pancreatic aspirates, possibly impacting management.
(© 2020 American Cancer Society.)

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