-
Tytuł:
-
Attenuation of subcutaneous insulin induced amyloid mass in vivo using Lumbrokinase and Serratiopeptidase.
-
Autorzy:
-
Metkar SK; Medical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Chettinad Health City, Kelambakkam, Chennai 603103, India.
Girigoswami A; Medical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Chettinad Health City, Kelambakkam, Chennai 603103, India.
Vijayashree R; Department of Pathology, Chettinad Hospital and Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Chettinad Health City, Kelambakkam, Chennai 603103, India.
Girigoswami K; Medical Bionanotechnology, Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Chettinad Health City, Kelambakkam, Chennai 603103, India. Electronic address: .
-
Źródło:
-
International journal of biological macromolecules [Int J Biol Macromol] 2020 Nov 15; Vol. 163, pp. 128-134. Date of Electronic Publication: 2020 Jun 29.
-
Typ publikacji:
-
Journal Article
-
Język:
-
English
-
Imprint Name(s):
-
Publication: Amsterdam : Elsevier
Original Publication: Guildford, Eng., IPC Science and Technology Press.
-
MeSH Terms:
-
Endopeptidases/*pharmacology
Insulin/*adverse effects
Peptide Hydrolases/*pharmacology
Plaque, Amyloid/*metabolism
Plaque, Amyloid/*prevention & control
Protein Aggregation, Pathological/*metabolism
Protein Aggregation, Pathological/*prevention & control
Animals ; Benzothiazoles/chemistry ; Congo Red/chemistry ; Disease Models, Animal ; Injection Site Reaction/pathology ; Injection Site Reaction/prevention & control ; Injections, Subcutaneous ; Insulin/administration & dosage ; Male ; Microscopy, Fluorescence ; Microscopy, Polarization ; Plaque, Amyloid/chemically induced ; Plaque, Amyloid/pathology ; Protein Aggregation, Pathological/chemically induced ; Protein Aggregation, Pathological/pathology ; Rats ; Rats, Wistar ; Subcutaneous Tissue/drug effects ; Subcutaneous Tissue/pathology
-
Contributed Indexing:
-
Keywords: Amyloid degradation; Amyloidosis; Congo red; Insulin amyloid mass; Wistar rat
-
Substance Nomenclature:
-
0 (Benzothiazoles)
0 (Insulin)
2390-54-7 (thioflavin T)
3U05FHG59S (Congo Red)
EC 3.4.- (Endopeptidases)
EC 3.4.- (Peptide Hydrolases)
EC 3.4.99.- (lumbrokinase)
NL053ABE4J (serratiopeptidase)
-
Entry Date(s):
-
Date Created: 20200703 Date Completed: 20210409 Latest Revision: 20210409
-
Update Code:
-
20240105
-
DOI:
-
10.1016/j.ijbiomac.2020.06.256
-
PMID:
-
32615214
-
The protein misfolded structure called amyloids is related with extensive range of pathologies like local amyloidosis and neurodegenerative diseases. Several studies have reported the potential of insulin to generate local amyloidosis under certain state. Reports also showed that fibrils of insulin generated local amyloid mass due to continuous subcutaneous injection in mouse as well as rat. The present study was designed to examine the consequence of insulin fibril injections in rats, as well as the ability of enzymes, Lumbrokinase (LK) and Serratiopeptidase (SP) in diminishing this amyloid mass progression. The results showed that insulin fibrils generated amyloid masses in rats after subcutaneous injection for two weeks which was significantly condensed in size for the groups injected with insulin fibrils combined with LK or SP. At higher doses of LK and SP, the absence of amyloid structure was observed in histopathological studies. Light microscopy, polarized microscopy as well as Lumia live in vivo imaging system was used to analyze the results. In conclusion, the overall outcome of this study showed the anti-amyloid potential of enzyme LK and SP in the attenuation of local amyloidosis.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
