IGFBP5 enhances the dentinogenesis potential of dental pulp stem cells via JNK and ErK signalling pathways.

Szczegóły
Abstrakt

Tytuł:: IGFBP5 enhances the dentinogenesis potential of dental pulp stem cells via JNK and ErK signalling pathways.
Autorzy:: Hao J; Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China.; Department of Endodontics, Capital Medical University School of Stomatology, Beijing, China.
Yang H; Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China.
Cao Y; Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China.
Zhang C; Department of Endodontics, Capital Medical University School of Stomatology, Beijing, China.
Fan Z; Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China.
Źródło:: Journal of oral rehabilitation [J Oral Rehabil] 2020 Dec; Vol. 47 (12), pp. 1557-1565. Date of Electronic Publication: 2020 Jul 22.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Oxford, Blackwell Scientific Publications.
MeSH Terms:: MAP Kinase Signaling System*
Osteogenesis*
Animals ; Cell Differentiation ; Cells, Cultured ; Dental Pulp ; Dentinogenesis ; Humans ; Mice ; Mice, Nude ; Stem Cells
References:: Bakhtiar H, Mazidi SA, Mohammadi AS et al The role of stem cell therapy in regeneration of dentine-pulp complex: a systematic review. Prog Biomater. 2018;7(4):249-268.
Stambolsky C, Rodriguez-Benitez S, Gutierrez-Perez JL et al Histologic characterization of regenerated tissues after pulp revascularization of immature dog teeth with apical periodontitis using tri-antibiotic paste and platelet-rich plasma. Arch Oral Biol. 2016;71:122-128.
Wang X, Thibodeau B, Trope M et al Histologic characterization of regenerated tissues in canal space after the revitalization/revascularization procedure of immature dog teeth with apical periodontitis. J Endod. 2010;36(1):56-63.
Cooper PR, Takahashi Y, Graham LW et al Inflammation-regeneration interplay in the dentine-pulp complex. J Dent. 2010;38(9):687-697.
Sonoyama W, Liu Y, Fang D et al Mesenchymal stem cell-mediated functional tooth regeneration in swine. PLoS ONE. 2006;1:e79.
Saito MT, Silverio KG, Casati MZ et al Tooth-derived stem cells: update and perspectives. World J Stem Cells. 2015;7(2):399-407.
Simon SR, Berdal A, Cooper PR et al Dentin-pulp complex regeneration: from lab to clinic. Adv Dent Res. 2011;23(3):340-345.
Zhang W, Yelick PC. Vital pulp therapy-current progress of dental pulp regeneration and revascularization. Int J Dent. 2010;2010:856087.
Ding H, Wu T. Insulin-like growth factor binding proteins in autoimmune diseases. Front Endocrinol (Lausanne). 2018;9:499.
Gullu G, Karabulut S, Akkiprik M. Functional roles and clinical values of insulin-like growth factor-binding protein-5 in different types of cancers. Chin J Cancer. 2012;31(6):266-280.
Miyakoshi N, Richman C, Kasukawa Y et al Evidence that IGF-binding protein-5 functions as a growth factor. J Clin Invest. 2001;107(1):73-81.
Tripathi G, Salih DA, Drozd AC et al IGF-independent effects of insulin-like growth factor binding protein-5 (Igfbp5) in vivo. FASEB J. 2009;23(8):2616-2626.
Baxter RC. Nuclear actions of insulin-like growth factor binding protein-3. Gene. 2015;569(1):7-13.
Sun M, Long J, Yi Y et al Importin alpha-importin beta complex mediated nuclear translocation of insulin-like growth factor binding protein-5. Endocr J. 2017;64(10):963-975.
Fleming JM, Brandimarto JA, Cohick WS. The mitogen-activated protein kinase pathway tonically inhibits both basal and IGF-I-stimulated IGF-binding protein-5 production in mammary epithelial cells. J Endocrinol. 2007;194(2):349-359.
Wang Y, Jia Z, Diao S et al IGFBP5 enhances osteogenic differentiation potential of periodontal ligament stem cells and Wharton's jelly umbilical cord stem cells, via the JNK and MEK/Erk signaling pathways. Cell Prolif. 2016;49(5):618-627.
Liu D, Wang Y, Jia Z et al Demethylation of IGFBP5 by Histone Demethylase KDM6B Promotes Mesenchymal Stem Cell-Mediated Periodontal Tissue Regeneration by Enhancing Osteogenic Differentiation and Anti-Inflammation Potentials. Stem Cells. 2015;33(8):2523-2536.
Han N, Zhang F, Li G et al Local application of IGFBP5 protein enhanced periodontal tissue regeneration via increasing the migration, cell proliferation and osteo/dentinogenic differentiation of mesenchymal stem cells in an inflammatory niche. Stem Cell Res Ther. 2017;8(1):210.
Cao Y, Liu Z, Xie Y et al Adenovirus-mediated transfer of hepatocyte growth factor gene to human dental pulp stem cells under good manufacturing practice improves their potential for periodontal regeneration in swine. Stem Cell Res Ther. 2015;6:249.
Yang H, Liang Y, Cao Y et al Homeobox C8 inhibited the osteo-/dentinogenic differentiation and migration ability of stem cells of the apical papilla via activating KDM1A. J Cell Physiol. 2020.
Hu L, Gao Z, Xu J et al Decellularized Swine Dental Pulp as a Bioscaffold for Pulp Regeneration. Biomed Res Int. 2017;2017:9342714.
Song JS, Takimoto K, Jeon M et al Decellularized human dental pulp as a scaffold for regenerative endodontics. J Dent Res. 2017;96(6):640-646.
Murray PE, Garcia-Godoy F, Hargreaves KM. Regenerative endodontics: a review of current status and a call for action. J Endod. 2007;33(4):377-390.
Gronthos S, Mankani M, Brahim J et al Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A. 2000;97(25):13625-13630.
Yao L, Flynn N. Dental pulp stem cell-derived chondrogenic cells demonstrate differential cell motility in type I and type II collagen hydrogels. Spine J. 2018;18(6):1070-1080.
Gronthos S, Brahim J, Li W et al Stem cell properties of human dental pulp stem cells. J Dent Res. 2002;81(8):531-535.
Shui C, Scutt A. Mild heat shock induces proliferation, alkaline phosphatase activity, and mineralization in human bone marrow stromal cells and Mg-63 cells in vitro. J Bone Miner Res. 2001;16(4):731-741.
Seo MS, Hwang KG, Lee J et al The effect of mineral trioxide aggregate on odontogenic differentiation in dental pulp stem cells. J Endod. 2013;39(2):242-248.
Suzuki S, Haruyama N, Nishimura F et al Dentin sialophosphoprotein and dentin matrix protein-1: Two highly phosphorylated proteins in mineralized tissues. Arch Oral Biol. 2012;57(9):1165-1175.
Narayanan K, Ramachandran A, Hao J et al Dual functional roles of dentin matrix protein 1. Implications in biomineralization and gene transcription by activation of intracellular Ca2+ store. J Biol Chem. 2003;278(19):17500-17508.
Guo S, Lim D, Dong Z et al Dentin sialophosphoprotein: a regulatory protein for dental pulp stem cell identity and fate. Stem Cells Dev. 2014;23(23):2883-2894.
Wu L, Zhu F, Wu Y et al Dentin sialophosphoprotein-promoted mineralization and expression of odontogenic genes in adipose-derived stromal cells. Cells Tissues Organs. 2008;187(2):103-112.
Kuemmerle JF, Zhou H. Insulin-like growth factor-binding protein-5 (IGFBP-5) stimulates growth and IGF-I secretion in human intestinal smooth muscle by Ras-dependent activation of p38 MAP kinase and Erk1/2 pathways. J Biol Chem. 2002;277(23):20563-20571.
Sun Y, Liu WZ, Liu T et al Signaling pathway of MAPK/ERK in cell proliferation, differentiation, migration, senescence and apoptosis. J Recept Signal Transduct Res. 2015;35(6):600-604.
Grant Information:: 81200783 National Natural Science Foundation of China; Dongcheng District Outstanding Talents Training and Assistance; 19-09-01 Discipline Construction Foundation of Beijing Stomatological Hospital; 81625005 National Natural Science Foundation of China
Contributed Indexing:: Keywords: Insulin-like growth factor-binding protein 5; dental pulp stem cells; dentinogenesis; dentinogenic differentiation
Entry Date(s):: Date Created: 20200706 Date Completed: 20201127 Latest Revision: 20201127
Update Code:: 20240104
DOI:: 10.1111/joor.13047
PMID:: 32623775
: Czasopismo naukowe

Full Text Finder

Background: Dental stem cell transplantation has become a new method for tooth tissue regeneration. However, its molecular mechanism of the dentinogenic differentiation is still unclear, limited its application. Our previous studies found that insulin-like growth factor-binding protein 5 (IGFBP5) can promote the osteogenic differentiation of periodontal ligament stem cells and the regeneration of periodontal tissues. This study aims to clarify the effect and mechanism of IGFBP5 on the dentinogenesis of dental pulp stem cells (DPSCs).
Objective and Methods: Lentiviral IGFBP5 shRNA was used to knock-down of IGFBP5. And recombinant human IGFBP5 protein (rhIGFBP5) was used to treat DPSCs. Alkaline phosphatase (ALP) staining, Alizarin red staining, quantitative calcium analysis, real-time RT-PCR and Western Blot were used to detect dentinogenic differentiation markers and related signalling pathways. Transplantation in nude mice was used to detect the dentin regeneration in vivo.
Results: Depletion of IGFBP5 inhibited ALP activity and the mineralisation and reduced the expressions of osteo/dentinogenic differentiation markers BSP, DMP-1 and DSPP in DPSCs. 0.05 ng/mL rhIGFBP5 promoted ALP activity, the mineralisation and the expressions of BSP, DMP-1 and DSPP in DPSCs. In addition, 0.05 ng/mL rhIGFBP5 could promote DPSC-mediated dentin-like tissues formation in vivo. Western blot results showed that IGFBP5 activated JNK and Erk signalling pathways in DPSCs. Furthermore, inhibition of JNK pathway by SP600125, the expression of p-JNK and p-Erk was reduced, while inhibition of Erk pathway by PD98059, only p-Erk expression was decreased.
Conclusions: Our results demonstrated that IGFBP5 could promote the dentinogenic differentiation and dentinogenesis potential of DPSCs via JNK and ErK signalling pathways.
(© 2020 John Wiley & Sons Ltd.)

Informacja