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Tytuł pozycji:

Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT-qPCR primer-probe sets.

Tytuł :
Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT-qPCR primer-probe sets.
Autorzy :
Vogels CBF; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA. .
Brito AF; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA.
Wyllie AL; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA.
Fauver JR; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA.
Ott IM; Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT, USA.
Kalinich CC; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA.
Petrone ME; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA.
Casanovas-Massana A; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA.
Catherine Muenker M; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA.
Moore AJ; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA.
Klein J; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Lu P; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Lu-Culligan A; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Jiang X; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Kim DJ; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Kudo E; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Mao T; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Moriyama M; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Oh JE; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Park A; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Silva J; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Song E; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Takahashi T; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Taura M; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Tokuyama M; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Venkataraman A; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Weizman OE; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Wong P; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Yang Y; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.
Cheemarla NR; Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT, USA.
White EB; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA.
Lapidus S; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA.
Earnest R; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA.
Geng B; Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, New Haven, CT, USA.
Vijayakumar P; Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, New Haven, CT, USA.
Odio C; Department of Medicine, Northeast Medical Group, Yale-New Haven Health, New Haven, CT, USA.
Fournier J; Department of Medicine, Section of Infectious Diseases, Yale University School of Medicine, New Haven, CT, USA.
Bermejo S; Department of Internal Medicine, Section of Pulmonary, Critical Care, and Sleep Medicine, Yale University School of Medicine, New Haven, CT, USA.
Farhadian S; Department of Medicine, Section of Infectious Diseases, Yale University School of Medicine, New Haven, CT, USA.
Dela Cruz CS; Department of Internal Medicine, Section of Pulmonary, Critical Care, and Sleep Medicine, Yale University School of Medicine, New Haven, CT, USA.
Iwasaki A; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA.
Ko AI; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA.
Landry ML; Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT, USA.; Department of Medicine, Section of Infectious Diseases, Yale University School of Medicine, New Haven, CT, USA.; Clinical Virology Laboratory, Yale-New Haven Hospital, New Haven, CT, USA.
Foxman EF; Department of Immunobiology, Yale University School of Medicine, New Haven, CT, USA.; Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT, USA.
Grubaugh ND; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA. .
Pokaż więcej
Źródło :
Nature microbiology [Nat Microbiol] 2020 Oct; Vol. 5 (10), pp. 1299-1305. Date of Electronic Publication: 2020 Jul 10.
Typ publikacji :
Comparative Study; Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
Język :
English
Imprint Name(s) :
Original Publication: [London] : Nature Publishing Group, [2016]-
MeSH Terms :
Betacoronavirus/*genetics
Clinical Laboratory Techniques/*methods
Coronavirus Infections/*diagnosis
Coronavirus Infections/*virology
Pneumonia, Viral/*diagnosis
Pneumonia, Viral/*virology
RNA, Viral/*analysis
RNA, Viral/*genetics
Reverse Transcriptase Polymerase Chain Reaction/*methods
Betacoronavirus/isolation & purification ; Clinical Laboratory Techniques/statistics & numerical data ; Coronavirus Infections/epidemiology ; Genetic Variation ; Genome, Viral ; Humans ; Molecular Probe Techniques/statistics & numerical data ; Pandemics ; Pneumonia, Viral/epidemiology ; RNA/genetics ; RNA Probes/genetics ; Reverse Transcriptase Polymerase Chain Reaction/statistics & numerical data ; Sensitivity and Specificity
Substance Nomenclature :
0 (RNA Probes)
0 (RNA primers)
0 (RNA, Viral)
63231-63-0 (RNA)
SCR Disease Name :
COVID-19
Entry Date(s) :
Date Created: 20200712 Date Completed: 20201001 Latest Revision: 20201001
Update Code :
20201023
DOI :
10.1038/s41564-020-0761-6
PMID :
32651556
Czasopismo naukowe
The recent spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exemplifies the critical need for accurate and rapid diagnostic assays to prompt clinical and public health interventions. Currently, several quantitative reverse transcription-PCR (RT-qPCR) assays are being used by clinical, research and public health laboratories. However, it is currently unclear whether results from different tests are comparable. Our goal was to make independent evaluations of primer-probe sets used in four common SARS-CoV-2 diagnostic assays. From our comparisons of RT-qPCR analytical efficiency and sensitivity, we show that all primer-probe sets can be used to detect SARS-CoV-2 at 500 viral RNA copies per reaction. The exception for this is the RdRp-SARSr (Charité) confirmatory primer-probe set which has low sensitivity, probably due to a mismatch to circulating SARS-CoV-2 in the reverse primer. We did not find evidence for background amplification with pre-COVID-19 samples or recent SARS-CoV-2 evolution decreasing sensitivity. Our recommendation for SARS-CoV-2 diagnostic testing is to select an assay with high sensitivity and that is regionally used, to ease comparability between outcomes.

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