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Tytuł:
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Sulfated N-acetylglucosamino-glucuronopyranosyl-arabinopyranan from seafood Amphioctopus neglectus attenuates angiotensin-II prompted cardiac hypertrophy.
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Autorzy:
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Chakraborty K; Marine Biotechnology Division, Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin 682018, Kerala, India. Electronic address: .
Krishnan S; Marine Biotechnology Division, Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin 682018, Kerala, India.
Joy M; Marine Biotechnology Division, Central Marine Fisheries Research Institute, Ernakulam North, P.B. No. 1603, Cochin 682018, Kerala, India.
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Źródło:
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International journal of biological macromolecules [Int J Biol Macromol] 2020 Nov 15; Vol. 163, pp. 1223-1232. Date of Electronic Publication: 2020 Jul 09.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Amsterdam : Elsevier
Original Publication: Guildford, Eng., IPC Science and Technology Press.
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MeSH Terms:
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Acetylglucosamine/*chemistry
Acetylglucosamine/*pharmacology
Seafood/*analysis
Sulfates/*chemistry
Acetylglucosamine/isolation & purification ; Angiotensin II/adverse effects ; Angiotensin II/metabolism ; Animals ; Antihypertensive Agents/chemistry ; Antihypertensive Agents/isolation & purification ; Antihypertensive Agents/pharmacology ; Antioxidants/chemistry ; Antioxidants/isolation & purification ; Antioxidants/pharmacology ; Cardiomegaly/drug therapy ; Cardiomegaly/etiology ; Cardiomegaly/metabolism ; Cephalopoda/chemistry ; Disease Susceptibility ; Humans ; Magnetic Resonance Spectroscopy ; Molecular Docking Simulation ; Molecular Dynamics Simulation ; Monosaccharides/chemistry ; Peptidyl-Dipeptidase A/metabolism ; Polysaccharides/chemistry ; Polysaccharides/isolation & purification ; Structure-Activity Relationship
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Contributed Indexing:
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Keywords: Amphioctopus neglectus; Angiotensin-II induced cardiac hypertrophy; Sulfated N-acetylglucosamino-glucuronopyranosyl-arabinopyranan
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Substance Nomenclature:
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0 (Antihypertensive Agents)
0 (Antioxidants)
0 (Monosaccharides)
0 (Polysaccharides)
0 (Sulfates)
11128-99-7 (Angiotensin II)
EC 3.4.15.1 (Peptidyl-Dipeptidase A)
V956696549 (Acetylglucosamine)
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Entry Date(s):
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Date Created: 20200712 Date Completed: 20210402 Latest Revision: 20210402
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Update Code:
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20240105
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DOI:
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10.1016/j.ijbiomac.2020.07.037
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PMID:
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32652154
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Angiotensin converting enzyme (ACE) is a multifunctional enzyme involved in translation of angiotensin-I (Ang I ) to vasoconstrictor angiotensin-II (Ang II ). A sulfated N-acetylglucosamino-glucuronopyranosyl-arabinopyranan characterized as poly-[(2-methoxy-β-arabinopyranosyl)-(1 → 3)-(β-glucurono)-(1 → 4)-(2-acetamido-2-deoxy-3,6-di-O-sulfonato-β-glucopyranose)] was purified and reported first time from the edible portion of Amphioctopus neglectus and evaluated for various pharmacological properties. The polysaccharide exhibited potential ACE attenuation property (IC 50 0.11 mg mL -1 ), whereas molecular docking simulations displayed its efficient binding at the ACE active site with lesser inhibitory constant (K i ) of 17.36 nM and binding energy (-10.59 kcal mol -1 ). The in-vitro analysis showed that the studied polysacharide attenuated Ang II prompted cardiac hypertrophy at 50 μg mL -1 in the cardiomyoblast cells, whereas 48% reduction in cellular surface area with extended viability could be correlated with anti-hypertrophic properties of the studied polysaccharide. The sulfated N-acetylglucosamino-glucuronopyranosyl-arabinopyranan purified from A. neglectus could function as a prospective functional lead against the pathophysiological conditions leading to hypertension.
Competing Interests: Declaration of competing interest No conflict of interest was reported by the authors.
(Copyright © 2020 Elsevier B.V. All rights reserved.)