Recombinant Lactococcus Lactis Displaying Omp31 Antigen of Brucella melitensis Can Induce an Immunogenic Response in BALB/c Mice.

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Tytuł:: Recombinant Lactococcus Lactis Displaying Omp31 Antigen of Brucella melitensis Can Induce an Immunogenic Response in BALB/c Mice.
Autorzy:: Shirdast H; Department of Medical Biotechnology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Ebrahimzadeh F; Department of Medical Biotechnology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Taromchi AH; Department of Medical Biotechnology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. .; Cancer Gene Therapy Research Center, Zanjan University of Medical Sciences, Zanjan, Iran. .
Mortazavi Y; Department of Medical Biotechnology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.; Cancer Gene Therapy Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.
Esmaeilzadeh A; Cancer Gene Therapy Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.; Department of Immunology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Sekhavati MH; Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Nedaei K; Department of Medical Biotechnology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Mirabzadeh E; Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Źródło:: Probiotics and antimicrobial proteins [Probiotics Antimicrob Proteins] 2021 Feb; Vol. 13 (1), pp. 80-89.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: New York, NY. : Springer
MeSH Terms:: Bacterial Outer Membrane Proteins*/genetics
Bacterial Outer Membrane Proteins*/immunology
Brucella Vaccine*/genetics
Brucella Vaccine*/immunology
Brucellosis*/immunology
Brucellosis*/prevention & control
Lactococcus lactis*/genetics
Lactococcus lactis*/immunology
Microorganisms, Genetically-Modified*/genetics
Microorganisms, Genetically-Modified*/immunology
Brucella melitensis/*genetics
Animals ; Brucella melitensis/immunology ; Female ; Mice ; Mice, Inbred BALB C
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Contributed Indexing:: Keywords: Brucella; Brucellosis; Lactococcus lactis; Omp31; Vaccine; pNZ7021
Substance Nomenclature:: 0 (Bacterial Outer Membrane Proteins)
0 (Brucella Vaccine)
0 (Omp31 protein, Brucella melitensis)
Entry Date(s):: Date Created: 20200715 Date Completed: 20210616 Latest Revision: 20210616
Update Code:: 20240105
DOI:: 10.1007/s12602-020-09684-1
PMID:: 32661939
: Czasopismo naukowe

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Since Brucella infection mostly occurs through the mucosal surfaces, immune response induced by vaccine that is delivered by a way of mucosal route can be drastically enhanced to control the brucellosis. Omp31is the major outer membrane protein of Brucella, and is considered as a protective antigen against Brucella infection. Accordingly, Lactococcus lactis has been used as an antigen-delivering vector to develop a vaccine-induced mucosal response for having a safer vaccination against brucellosis. A designed omp31 gene fused to the usp45 signal peptide and M6 cell wall anchor was sub cloned in the pNZ7021 expression vector, and a recombinant L. lactis displaying Omp31 was constructed. Omp31 protein expression was confirmed using Western blotting and immunofluorescence analysis. Animals were orally and intraperitoneally immunized with live or killed L. lactis expressing Omp31, respectively. The humoral and cellular immune responses were evaluated by measuring the specific cytokines and antibodies. sIgA, serum IgA, IgM, and total IgG antibodies significantly increased in the mice immunized with live recombinant L. lactis expressing Omp31 and also serum IgM, and total IgG antibodies significantly increased in mice immunized with killed recombinant L. lactis expressing Omp31. Among IgG subtypes, IgG2a response was significantly higher in both groups compared to IgG1. In mice groups immunized with recombinant L. lactis, the IFN-γ and IL-10 level elevated; however, there was no change in the level of IL-4. These results indicated that recombinants L. lactis induce both humoral and cellular immune responses in mice, and also vaccines based on L. lactis-derived live carriers are promising interventions against Brucella melitensis infections.

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