Ortho-silicic Acid Inhibits RANKL-Induced Osteoclastogenesis and Reverses Ovariectomy-Induced Bone Loss In Vivo.

Tytuł:: Ortho-silicic Acid Inhibits RANKL-Induced Osteoclastogenesis and Reverses Ovariectomy-Induced Bone Loss In Vivo.
Autorzy:: Ma W; Department of Spine Surgery, Qilu Hospital of Shandong University, Jinan, China.
Wang F; Department of Spine Surgery, Qilu Hospital of Shandong University, Jinan, China.
You Y; Department of Spine Surgery, Qilu Hospital of Shandong University, Jinan, China.
Wu W; Department of Spine Surgery, Qilu Hospital of Shandong University, Jinan, China.
Chi H; Department of traumatic Orthopedics, West Branch of Shandong Provincial Hospital, Jinan, China.
Jiao G; Department of Spine Surgery, Qilu Hospital of Shandong University, Jinan, China.
Zhang L; Department of Spine Surgery, Qilu Hospital of Shandong University, Jinan, China.
Zhou H; Department of Spine Surgery, Linyi Central Hospital, Linyi, China.
Wang H; Department of Spine Surgery, Qilu Hospital of Shandong University, Jinan, China.
Chen Y; Department of Spine Surgery, Qilu Hospital of Shandong University, Jinan, China. .
Źródło:: Biological trace element research [Biol Trace Elem Res] 2021 May; Vol. 199 (5), pp. 1864-1876. Date of Electronic Publication: 2020 Jul 16.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: [London, Clifton, N. J.] Humana Press.
MeSH Terms:: Bone Resorption*/drug therapy
RANK Ligand*
Animals ; Cell Differentiation ; Female ; Humans ; NF-kappa B ; Osteogenesis ; Ovariectomy ; Rats ; Silicic Acid
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Grant Information:: 2017GSF18160 Department of Science and Technology of Shandong Province; 2018WS328 Shandong Province Medical and Health Science and Technology Development Plan; 2017QLQN05 Qilu Hospital Youth Fund
Contributed Indexing:: Keywords: Ortho-silicic acid; Osteoclast; Osteoporosis; Ovariectomy; RANKL
Substance Nomenclature:: 0 (NF-kappa B)
0 (RANK Ligand)
1343-98-2 (Silicic Acid)
Entry Date(s):: Date Created: 20200718 Date Completed: 20210618 Latest Revision: 20210618
Update Code:: 20240105
DOI:: 10.1007/s12011-020-02286-6
PMID:: 32676940
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Numerous experiments in vitro and in vivo have shown that an appropriate increase intake of silicon can facilitate the synthesis of collagen and its stabilization and promote the differentiation and mineralization of osteoblasts. In this study, we examined whether ortho-silicic acid restrains the differentiation of osteoclast through the receptor activator of nuclear factor κB ligand (RANKL)/receptor activator of nuclear factor κB (RANK)/osteoprotegerin (OPG) signaling pathway by investigating its effect in vitro and in vivo. Bone marrow macrophage (BMM) cells were isolated and cultured with or without ortho-silicic acid, and then TRAP staining and immunofluorescence were performed to detect the differentiation of osteoclast. The RANKL-induced osteoclast marker gene and protein expression including c-Fos, nuclear factor of activated T cells cl (NFATcl), tumor necrosis factor receptor-associated factor 6 (TRAF6), nuclear factor kappa B P50 (NF-κB P50), NF-κB P52, RANK, integrin β3, cathepsin K (CTSK), DC-STAMP, and TRAP were quantitatively detected by western blot and RT-PCR. Ovariectomized (OVX) rats were injected with ortho-silicic acid (OVX+Si group) and normal saline (OVX group), and sham-operated rats were injected with normal saline (Sham group). And micro-CT, H&E, and TRAP staining, ELISA, and western blot were performed. Ortho-silicic acid could inhibit the differentiation of osteoclast, and the marker genes and proteins were decreased. The OVX-induced bone loss could be reversed by ortho-silicic acid. Our finding demonstrated that ortho-silicic acid suppresses RANKL-induced osteoclastogenesis and has potential value as a therapeutic agent for OVX-induced bone loss.

Erratum in: Biol Trace Elem Res. 2020 Sep 7;:. (PMID: 32893332)

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