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Tytuł:
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ACTH 4-10 protects the ADR-injured podocytes by stimulating B lymphocytes to secrete interleukin-10.
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Autorzy:
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Wang K; Department of Surgical Urology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
Du H; Suzhou Ninth People's Hospital, Suzhou, Jiangsu, China.
Chen Z; Department of Surgical Urology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
Lu H; Department of Surgical Urology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
Xu R; Department of Surgical Urology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.
Xue D; Department of Surgical Urology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China. Electronic address: .
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Źródło:
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International immunopharmacology [Int Immunopharmacol] 2020 Oct; Vol. 87, pp. 106769. Date of Electronic Publication: 2020 Jul 15.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Amsterdam ; New York : Elsevier Science, c2001-
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MeSH Terms:
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Antibiotics, Antineoplastic*
Doxorubicin*
Adrenocorticotropic Hormone/*pharmacology
B-Lymphocytes/*drug effects
Peptide Fragments/*pharmacology
Podocytes/*drug effects
Animals ; B-Lymphocytes/metabolism ; Cells, Cultured ; Interleukin-10/metabolism ; Mice
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Contributed Indexing:
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Keywords: ACTH(4-10); B cell; IL-10; Podocyte
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Substance Nomenclature:
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0 (Antibiotics, Antineoplastic)
0 (IL10 protein, mouse)
0 (Peptide Fragments)
130068-27-8 (Interleukin-10)
80168379AG (Doxorubicin)
9002-60-2 (Adrenocorticotropic Hormone)
J48AU3I790 (ACTH (4-10))
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Entry Date(s):
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Date Created: 20200719 Date Completed: 20210527 Latest Revision: 20210527
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Update Code:
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20240105
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DOI:
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10.1016/j.intimp.2020.106769
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PMID:
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32682256
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Objectives: In the present study, we aimed to assess whether adrenocorticotropic hormone (ACTH) could protect the podocytes from adriamycin (ADR)-induced injury by stimulating B lymphocytes to secrete the associated cytokines.
Methods: Proliferation assay was used to assess the proliferation and activity of podocytes. Enzyme-linked immunosorbent assay was used to examine the secretion of IL-10 and IL-4. TUNEL apoptosis detection kit was used to detect the apoptosis of podocytes. Real-time PCR and Western blotting analysis were used to examine the expressions of nephrin and podocin at the mRNA and protein levels.
Results: Compared with the normal control group, the podocyte proliferation of ADR group was significantly inhibited. However, compared with the ADR group, the podocyte proliferation of the supernatant (1 µg/L, 10 µg/L or 100 µg/L ACTH 4-10 ) + ADR groups was generally increased, and the pro-proliferative effect of the supernatant containing 10 µg/L ACTH 4-10 was the highest. Moreover, we found that after B lymphocytes were intervened by 10 µg/L ACTH4-10, the IL-10 level in the cell supernatant was significantly elevated (p < 0.05). When anti-IL-10R was added, the podocyte proliferation of the supernatant (10 µg/L ACTH 4-10 ) + ADR group was significantly inhibited. Furthermore, the supernatant of B cells stimulated with 10 µg/L ACTH 4-10 could better decrease the apoptosis rate of injured podocytes and increase the expressions of nephrin and podocin at the mRNA and protein levels by elevating the secretion of IL-10.
Conclusion: Compared with ACTH 4-10, the supernatant of B cells stimulated with ACTH 4-10 could better protect the podocytes from ADR-induced injury by elevating the secretion of IL-10.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
