The impact of vitamin D supplementation on VDR gene expression and body composition in monozygotic twins: randomized controlled trial.

Tytuł:: The impact of vitamin D supplementation on VDR gene expression and body composition in monozygotic twins: randomized controlled trial.
Autorzy:: Medeiros JFP; Department of Health Sciences, Federal University of Rio Grande Do Norte, Av. General Cordeiro de Farias, S/N - Petrópolis, Natal, RN, 59012-570, Brazil. .
de Oliveira Borges MV; Department of Health Sciences, Federal University of Rio Grande Do Norte, Av. General Cordeiro de Farias, S/N - Petrópolis, Natal, RN, 59012-570, Brazil.
Soares AA; Department of Health Sciences, Federal University of Rio Grande Do Norte, Av. General Cordeiro de Farias, S/N - Petrópolis, Natal, RN, 59012-570, Brazil.
Dos Santos JC; Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande Do Norte, Natal, RN, Brazil.
de Oliveira ABB; Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande Do Norte, Natal, RN, Brazil.
da Costa CHB; Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande Do Norte, Natal, RN, Brazil.
Cruz MS; Department of Health Sciences, Federal University of Rio Grande Do Norte, Av. General Cordeiro de Farias, S/N - Petrópolis, Natal, RN, 59012-570, Brazil.
Bortolin RH; Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil.
de Freitas RCC; Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil.
Dantas PMS; Department of Health Sciences, Federal University of Rio Grande Do Norte, Av. General Cordeiro de Farias, S/N - Petrópolis, Natal, RN, 59012-570, Brazil.
Hirata MH; Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil.
Silbiger VN; Graduate Program in Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande Do Norte, Natal, RN, Brazil.; Department of Clinical and Toxicological Analyses, Federal University of Rio Grande Do Norte, Natal, RN, Brazil.
Luchessi AD; Department of Health Sciences, Federal University of Rio Grande Do Norte, Av. General Cordeiro de Farias, S/N - Petrópolis, Natal, RN, 59012-570, Brazil.; Graduate Program in Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Rio Grande Do Norte, Natal, RN, Brazil.; Department of Clinical and Toxicological Analyses, Federal University of Rio Grande Do Norte, Natal, RN, Brazil.
Źródło:: Scientific reports [Sci Rep] 2020 Jul 20; Vol. 10 (1), pp. 11943. Date of Electronic Publication: 2020 Jul 20.
Typ publikacji:: Journal Article; Randomized Controlled Trial; Twin Study
Język:: English
Imprint Name(s):: Original Publication: London : Nature Publishing Group, copyright 2011-
MeSH Terms:: Dietary Supplements*
Twins, Monozygotic*
Body Composition/*drug effects
Gene Expression Regulation/*drug effects
Receptors, Calcitriol/*genetics
Vitamin D/*administration & dosage
Adult ; Biomarkers ; Female ; Humans ; Male ; Receptors, Calcitriol/metabolism ; Skin ; Sunlight ; Treatment Outcome ; Young Adult
References:: Bhatt, S. P. et al. Vitamin D supplementation in overweight/obese Asian Indian women with prediabetes reduces glycemic measures and truncal subcutaneous fat: a 78 weeks randomized placebo-controlled trial (PREVENT-WIN Trial). Sci Rep. 10(1), 1–13 (2020). (PMID: 10.1038/s41598-019-56847-4)
Cho, Y. Y. & Chung, Y. J. Vitamin D supplementation does not prevent the recurrence of Graves’ disease. Sci Rep. 10(1), 1–7 (2020). (PMID: 10.1038/s41598-019-56847-4)
Feldman, D., Krishnan, A. V., Swami, S., Giovannucci, E. & Feldman, B. J. The role of vitamin D in reducing cancer risk and progression. Nat. Rev. Cancer 14(5), 342–357 (2014). (PMID: 2470565210.1038/nrc3691)
Holick, M. F. et al. Evaluation, treatment, and prevention of vitamin D deficiency: an Endocrine Society clinical practice guideline. J. Clin. Endocrinol. Metab. 96(7), 1911–1930 (2011). (PMID: 2164636810.1210/jc.2011-0385)
Chun, R. F., Liu, P. T., Modlin, R. L., Adams, J. S. & Hewison, M. Impact of vitamin D on immune function: lessons learned from genome-wide analysis. Front. Physiol. 5, 151 (2014). (PMID: 24795646400099810.3389/fphys.2014.00151)
Dastan, F., Salamzadeh, J., Pourrashid, M. H., Edalatifard, M. & Eslaminejad, A. Effects of high-dose Vitamin D replacement on the serum levels of systemic inflammatory biomarkers in patients with acute exacerbation of chronic obstructive pulmonary disease. COPD 16(3–4), 278–283 (2019). (PMID: 3155091510.1080/15412555.2019.1666812)
Bouillon, R. et al. Vitamin D and human health: lessons from vitamin D receptor null mice. Endocr. Rev. 29(6), 726–776 (2008). (PMID: 18694980258338810.1210/er.2008-0004)
Christakos, S., Dhawan, P., Verstuyf, A., Verlinden, L. & Carmeliet, G. Vitamin D: metabolism, molecular mechanism of action, and pleiotropic effects. Physiol. Rev. 96(1), 365–408 (2016). (PMID: 2668179510.1152/physrev.00014.2015)
Gupta, G. K., Agrawal, T., DelCore, M. G., Mohiuddin, S. M. & Agrawal, D. K. Vitamin D deficiency induces cardiac hypertrophy and inflammation in epicardial adipose tissue in hypercholesterolemic swine. Exp. Mol. Pathol. 93(1), 82–90 (2012). (PMID: 22537546341127410.1016/j.yexmp.2012.04.006)
Abbassy, H. A., Elwafa, R. A. A. & Omar, O. M. Bone mineral density and vitamin D receptor genetic variants in egyptian children with beta thalassemia major on vitamin D supplementation. Mediterr. J. Hematol. I 11(1), e2019013 (2019).
Muscogiuri, G. et al. Sex Differences of vitamin D status across BMI classes: an observational prospective cohort study. Nutrients 11(12), 3034 (2019). (PMID: 695036310.3390/nu11123034)
Al-Daghri, N. M. et al. Efficacy of vitamin D supplementation according to vitamin D-binding protein polymorphisms. Nutrition 63, 148–154 (2019). (PMID: 3095938310.1016/j.nut.2019.02.003)
Yao, P. et al. A dose–response study of vitamin D3 supplementation in healthy Chinese: a 5-arm randomized, placebo-controlled trial. Eur. J. Nutr. 55(1), 383–392 (2016). (PMID: 2569435010.1007/s00394-015-0859-4)
Beiguelman, B. The Study of Twins. 1th (ed. Ribeirão, P), pp 20–22 (São Paulo, 2008).
Vukić, M. et al. Relevance of vitamin D receptor target genes for monitoring the vitamin D responsiveness of primary human cells. PLoS ONE 10(4), e0124339 (2015). (PMID: 25875760439514510.1371/journal.pone.0124339)
Carlberg, C. & Haq, A. The concept of the personal vitamin D response index. J. Steroid Biochem. Mol. Biol. 175, 12–17 (2018). (PMID: 2803476410.1016/j.jsbmb.2016.12.011)
Ooki, S. & Asaka, A. Zygosity diagnosis in young twins by questionnaire for twins’ mothers and twins’ self-reports. Twin Res. Hum. Genet. 7(1), 5–12 (2004). (PMID: 10.1375/13690520460741381)
Del Valle, H. B. et al. (eds) Dietary reference intakes for calcium and vitamin D (National Academies Press, Washington, 2011).
Hanwell, H. E. C. et al. Sun exposure questionnaire predicts circulating 25-hydroxyvitamin D concentrations in Caucasian hospital workers in southern Italy. J. Steroid Biochem. Mol. Biol. 121(1–2), 334–337 (2010). (PMID: 2029878210.1016/j.jsbmb.2010.03.023)
Fitzpatrick, T. B. The validity and practicality of sun-reactive skin types I through VI. Arch. Dermatol. 124(6), 869–871 (1988). (PMID: 337751610.1001/archderm.1988.01670060015008)
Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2 method. Methods 25(4), 402–408 (2001). (PMID: 1184660910.1006/meth.2001.1262)
Ferreira, C. E. S. et al. Consensus-reference ranges of vitamin D [25 (OH) D] from the Brazilian medical societies. Brazilian Society of Clinical Pathology/Laboratory Medicine (SBPC/ML) and Brazilian Society of Endocrinology and Metabolism (SBEM). J. Bras. Patol. Med. Lab. 53(6), 377–381 (2017). (PMID: 10.5935/1676-2444.20170060)
Bouillon, R. Comparative analysis of nutritional guidelines for vitamin D. Nat. Rev. Endocrinol. 13(8), 466 (2017). (PMID: 2838731810.1038/nrendo.2017.31)
Rajakumar, K., De Las Heras, J., Lee, S., Holick, M. F. & Arslanian, S. A. 25-Hydroxyvitamin D concentrations and in vivo insulin sensitivity and β-cell function relative to insulin sensitivity in black and white youth. Diabetes Care 35(3), 627–633 (2012). (PMID: 22238280332270110.2337/dc11-1825)
Wamberg, L. et al. Expression of vitamin D-metabolizing enzymes in human adipose tissue—the effect of obesity and diet-induced weight loss. Int. J. Obes. 37(5), 651–657 (2013). (PMID: 10.1038/ijo.2012.112)
Bassatne, A. Vitamin D supplementation in obesity and during weight loss: a review of randomized controlled trials. Metabolism 92, 193–205 (2019). (PMID: 3061594910.1016/j.metabol.2018.12.010)
Walsh, J. S., Bowels, S. & Evans, A. L. Vitamin D in obesity. Curr. Opin. Endocrinol. Diabetes Obes. 24, 389–394 (2017). (PMID: 2891513410.1097/MED.0000000000000371)
Karefylakis, C. et al. Effect of Vitamin D supplementation on body composition and cardiorespiratory fitness in overweight men—a randomized controlled trial. Endocrine 61(3), 388–397 (2018). (PMID: 29978375610523710.1007/s12020-018-1665-6)
Hind, K., Oldroyd, B. & Truscott, J. G. In vivo precision of the GE Lunar iDXA densitometer for the measurement of total body composition and fat distribution in adults. Eur. J. Clin. Nutr. 65(1), 140–142 (2011). (PMID: 2084217110.1038/ejcn.2010.190)
Fuller, J. C. Jr. et al. Vitamin D status affects strength gains in older adults supplemented with a combination of β-hydroxy-β-methylbutyrate, arginine, and lysine: a cohort study. J. Parenter. Enter. Nutr. 35(6), 757–762 (2011). (PMID: 10.1177/0148607111413903)
Sun, X. et al. Effect of vitamin D supplementation on body composition and physical fitness in healthy adults: a double-blind. Randomized Controlled Trial Ann. Nutr. Metab. 75, 231–237 (2019). (PMID: 3180113510.1159/000504873)
Nguyen, V. T. et al. Vitamin D, inflammation, and relations to insulin resistance in premenopausal women with morbid obesity. Obesity. 23(8), 1591–1597 (2015). (PMID: 2614830410.1002/oby.21131)
Landrier, J. F., Karkeni, E., Marcotorchino, J., Bonnet, L. & Tourniaire, F. Vitamin D modulates adipose tissue biology: possible consequences for obesity?. Proc. Nutr. Soc. 75(1), 38–46 (2016). (PMID: 2656433410.1017/S0029665115004164)
Trudler, D., Farfara, D. & Frenkel, D. Toll-like receptors expression and signaling in glia cells in neuroamyloidogenic diseases: towards future therapeutic application. Mediators Inflamm. 2010, 1–12 (2010). (PMID: 10.1155/2010/497987)
Carlberg, C. Nutrigenomics of vitamin D. Nutrients 11(3), 676 (2019). (PMID: 647087410.3390/nu11030676)
Castro, L. C. The endocrinological system vitamin D. Arq. Bras. Endocrinol. Metab. 55(8), 566–575 (2011). (PMID: 10.1590/S0004-27302011000800010)
Carlberg, C. Vitamin D: a micronutrient regulating genes. Curr. Pharm. Des. 25(15), 1740–1746 (2019). (PMID: 3129816010.2174/1381612825666190705193227)
Zeitelhofer, M. et al. Functional genomics analysis of vitamin D effects on CD4+ T cells in vivo in experimental autoimmune encephalomyelitis‬. Proc. Natl. Acad. Sci. 114(9), e1678–e1687 (2017). (PMID: 2819688410.1073/pnas.16157831145338504)
Han, J. et al. Risk factors for vitamin D deficiency in sickle cell disease. Br. J. Haematol. 181(6), 828–835 (2018). (PMID: 29767851600292910.1111/bjh.15270)
Lee, G. Y. et al. Differential effect of dietary vitamin D supplementation on natural killer cell activity in lean and obese mice. J. Nutr. Biochem. 55, 178–184 (2018). (PMID: 2952560910.1016/j.jnutbio.2018.01.004)
Anand, A. et al. Expression of vitamin D receptor and vitamin D status in patients with oral neoplasms and effect of vitamin D supplementation on quality of life in advanced cancer treatment. Contemporary Oncol. 21(2), 145 (2017).
Mazdeh, M. et al. Expression analysis of vitamin D signaling pathway genes in epileptic patients. J. Mol. Neurosci. 64(4), 551–558 (2018). (PMID: 2954959210.1007/s12031-018-1059-5)
Tomaszewska, M. et al. Role of vitamin D and its receptors in the pathophysiology of chronic rhinosinusitis. J. Am. Coll. Nutr. 38(2), 108–118 (2019). (PMID: 3038893510.1080/07315724.2018.1503102)
Munetsuna, E. et al. Anti-proliferative activity of 25-hydroxyvitamin D3 in human prostate cells. Mol. Cell Endocrinol. 382, 960–970 (2014). (PMID: 2429160910.1016/j.mce.2013.11.014)
Verone-Boyle, A. R. et al. Diet-derived 25-hydroxyvitamin D3 activates vitamin D receptor target gene expression and suppresses EGFR mutant non-small cell lung cancer growth in vitro and in vivo. Oncotarget 7, 995–1013 (2016). (PMID: 2665494210.18632/oncotarget.6493)
Giresi, P. G., Kim, J., McDaniell, R. M., Iyer, V. R. & Lieb, J. D. FAIRE (formaldehyde-assisted isolation of regulatory elements) isolates active regulatory elements from human chromatin. Genome Res. 17(6), 877–885 (2007). (PMID: 17179217189134610.1101/gr.5533506)
Breslavsky, A. et al. Effect of high doses of vitamin D on arterial properties, adiponectin, leptin and glucose homeostasis in type 2 diabetic patients. Clin. Nutr. 32(6), 970–975 (2013). (PMID: 2356163710.1016/j.clnu.2013.01.020)
Moghassemi, S. & Marjani, A. The effect of short-term vitamin D supplementation on lipid profile and blood pressure in post-menopausal women: a randomized controlled trial. Iran. J. Nurs. Midw. Res. 19(5), 517 (2014).
Ryu, O. H. et al. The effect of high-dose vitamin D supplementation on insulin resistance and arterial stiffness in patients with type 2 diabetes. Korean J. Intern. Med. 29(5), 620 (2014). (PMID: 25228838416472610.3904/kjim.2014.29.5.620)
Gepner, A. D. et al. A prospective randomized controlled trial of the effects of vitamin D supplementation on cardiovascular disease risk. PLoS ONE 7(5), e36617 (2012). (PMID: 22586483334673610.1371/journal.pone.0036617)
Gepner, A. D. et al. A randomized controlled trial of the effects of vitamin D supplementation on arterial stiffness and aortic blood pressure in native American women. Atherosclerosis 240(2), 526–528 (2015). (PMID: 25955191444155810.1016/j.atherosclerosis.2015.04.795)
Yiu, Y. F. et al. Randomized controlled trial of vitamin D supplement on endothelial function in patients with type 2 diabetes. Atherosclerosis. 227(1), 140–146 (2013). (PMID: 2329882410.1016/j.atherosclerosis.2012.12.013)
Marckmann, P. et al. Randomized controlled trial of cholecalciferol supplementation in chronic kidney disease patients with hypovitaminosis D. Nephrol. Dial. Transplant 27(9), 3523–3531 (2012). (PMID: 2282209210.1093/ndt/gfs138)
Dreyer, G. et al. Ergocalciferol and microcirculatory function in chronic kidney disease and concomitant vitamin d deficiency: an exploratory, double blind, randomised controlled trial. PLoS ONE 9(7), e99461 (2014). (PMID: 25006678409011710.1371/journal.pone.0099461)
Larsen, T., Mose, F. H., Bech, J. N., Hansen, A. B. & Pedersen, E. B. Effect of cholecalciferol supplementation during winter months in patients with hypertension: a randomized, placebo-controlled trial. Am. J. Hypertens. 25(11), 1215–1222 (2012). (PMID: 2285463910.1038/ajh.2012.111)
Mcgreevy, C., Barry, M., Bennett, K. & Williams, D. The effect of vitamin D replacement on arterial stiffness in an elderly community based population. Irish J. Med. Sci. 182, s234–s235 (2013).
Witham, M. D. et al. Cholecalciferol treatment to reduce blood pressure in older patients with isolated systolic hypertension: the VitDISH randomized controlled trial. JAMA Intern. Med. 173(18), 1672–1679 (2013). (PMID: 23939263)
Sadat-Ali, M., Al-Anii, F. M., Al-Turki, H. A., AlBadran, A. A. & AlShammari, S. A. M. Maintenance dose of vitamin D: How much is enough?. J. Bone Metab. 25(3), 161–164 (2018). (PMID: 30237995613565110.11005/jbm.2018.25.3.161)
Trehan, N., Afonso, L., Levine, D. L. & Levy, P. D. Vitamin D deficiency, supplementation, and cardiovascular health. Crit. Pathw. Cardiol. 16(3), 109–118 (2017). (PMID: 2874264810.1097/HPC.0000000000000122)
Mondul, A. M., Weinstein, S. J., Layne, T. M. & Albanes, D. Vitamin D and cancer risk and mortality: state of the science, gaps, and challenges. Epidemiol. Rev. 39(1), 28–48 (2017). (PMID: 28486651585803410.1093/epirev/mxx005)
Ali, M. I., Fawaz, L. A., Sedik, E. E., Nour, Z. A. & Elsayed, R. M. Vitamin D status in diabetic patients (type 2) and its relation to glycemic control & diabetic nephropathy. DMS Clin. Res. Rev. 13(3), 1971–1973 (2019).
Prietl, B., Treiber, G., Pieber, T. R. & Amrein, K. Vitamin D and immune function. Nutrients 5(7), 2502–2521 (2013). (PMID: 23857223373898410.3390/nu5072502)
Zheng, Y. et al. Meta-analysis of long-term vitamin D supplementation on overall mortality. PLoS ONE 8(12), e82109 (2013). (PMID: 24349197385778410.1371/journal.pone.0082109)
Lopez, Y. O. N., Pittas, A. G., Pratley, R. E. & Seyhan, A. A. Circulating levels of miR-7, miR-152 and miR-192 respond to vitamin D supplementation in adults with prediabetes and correlate with improvements in glycemic control. J. Nutr. Biochem. 49, 117–122 (2017). (PMID: 10.1016/j.jnutbio.2017.08.007)
Brennan, A. et al. Dendritic cells from human tissues express receptors for the immunoregulatory vitamin D3 metabolite, dihydroxycholecalciferol. Immunology 61(4), 457 (1987). (PMID: 28323071453440)
Bertolini, D. L. & Tzanno-Martins, C. Review: immunomodulatory effects of vitamin D. J. Bras. Nefrol. 22(3), 157–161 (2000).
Illescas-Montes, R., Melguizo-Rodríguez, L., Ruiz, C. & Costela-Ruiz, V. J. Vitamin D and autoimmune diseases. Life Sci. 233(15), 116744 (2019). (PMID: 3140131410.1016/j.lfs.2019.116744)
Balkwill, F. Tumor necrosis factor or tumor promoting factor?. Cytokine Growth Factor Rev. 13, 135–141 (2002). (PMID: 1190098910.1016/S1359-6101(01)00020-X)
Omidian, M. et al. Effects of vitamin D supplementation on circulatory YKL-40 and MCP-1 biomarkers associated with vascular diabetic complications: a randomized, placebo-controlled, double-blind clinical trial. DSM Clin. Res. Rev. 13(5), 2873–2877 (2019).
Borges, C. C., Salles, A. F., Bringhenti, I. & Mandarim-de-Lacerda, C. A. Aguila MB (2018) Vitamin D deficiency increases lipogenesis and reduces beta-oxidation in the liver of diet-induced obese mice. J. Nutr. Sci. Vitaminol. 64(2), 106–115 (2018). (PMID: 2971002810.3177/jnsv.64.106)
Substance Nomenclature:: 0 (Biomarkers)
0 (Receptors, Calcitriol)
1406-16-2 (Vitamin D)
Entry Date(s):: Date Created: 20200721 Date Completed: 20201221 Latest Revision: 20220418
Update Code:: 20240104
PubMed Central ID:: PMC7371728
DOI:: 10.1038/s41598-020-69128-2
PMID:: 32686744
: Czasopismo naukowe

Pełny tekst

Vitamin D supplementation is widely used. However, there is no consensus on the use and dosage of this supplement and the existing recommendations arise from studies based on the benefits that this nutrient can facilitate in bones. In addition, individual genetics can influence the response to supplementation, therefore, research involving monozygotic twins aims to reduce these differences in phenotypic responses. The objective of this randomised controlled study is to examine the effect of vitamin D supplementation on body composition and the expression of the vitamin D receptor (VDR) mRNA. An intervention was performed through supplementation with cholecalciferol at the concentration of 2000 IU in 90 healthy adult monozygotic twins (male or female pairs) for 2 months. The findings showed that serum vitamin D concentration increased by 65% and VDR gene expression sixty times (p = 0.001). Changes in body composition parameters were observed regarding body fat and lean mass. Our results indicate that an increase in serum vitamin D concentration may have potential therapeutic implications.

Zaloguj się, aby uzyskać dostęp do pełnego tekstu.

Informacja