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Tytuł pozycji:

Phase separation of Arabidopsis EMB1579 controls transcription, mRNA splicing, and development.

Tytuł :
Phase separation of Arabidopsis EMB1579 controls transcription, mRNA splicing, and development.
Autorzy :
Zhang Y; Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, China.
Li Z; Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, China.
Chen N; Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
Huang Y; Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, China.
Huang S; Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, China.
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Źródło :
PLoS biology [PLoS Biol] 2020 Jul 21; Vol. 18 (7), pp. e3000782. Date of Electronic Publication: 2020 Jul 21 (Print Publication: 2020).
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
Język :
English
Imprint Name(s) :
Original Publication: San Francisco, CA : Public Library of Science, [2003]-
MeSH Terms :
Transcription, Genetic*
Arabidopsis/*genetics
Arabidopsis/*growth & development
Arabidopsis Proteins/*metabolism
Calcium-Binding Proteins/*metabolism
Plant Development/*genetics
RNA Splicing/*genetics
Arabidopsis Proteins/genetics ; Calcium-Binding Proteins/genetics ; Cell Nucleus/metabolism ; Flowers/physiology ; Histones/metabolism ; Loss of Function Mutation ; Lysine/metabolism ; Methylation ; Nuclear Proteins/metabolism ; Phenotype ; Plant Roots/cytology ; Protein Binding ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Repetitive Sequences, Amino Acid
References :
Elife. 2018 Aug 16;7:. (PMID: 30113309)
Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):6050-3. (PMID: 11607479)
Nature. 2018 Jun;558(7709):318-323. (PMID: 29849146)
Cell. 2016 Jun 16;165(7):1686-1697. (PMID: 27212236)
Science. 2018 Sep 14;361(6407):1112-1115. (PMID: 30213912)
Cell. 2015 Aug 27;162(5):1066-77. (PMID: 26317470)
Plant J. 2015 Jul;83(1):121-32. (PMID: 25762111)
Trends Genet. 2011 Aug;27(8):295-306. (PMID: 21680045)
Plant Cell. 2011 Oct;23(10):3565-76. (PMID: 21984696)
Nature. 1990 Feb 1;343(6257):437-41. (PMID: 2137203)
Cytoskeleton (Hoboken). 2013 Oct;70(10):550-71. (PMID: 23761374)
Mol Cell. 2015 Mar 5;57(5):936-947. (PMID: 25747659)
Plant Cell. 2005 Jun;17(6):1723-36. (PMID: 15863515)
Plant Physiol. 2004 Jul;135(3):1206-20. (PMID: 15266054)
Plant Physiol. 2010 Feb;152(2):516-28. (PMID: 19783648)
Proc Natl Acad Sci U S A. 2015 Jun 9;112(23):7189-94. (PMID: 26015579)
Mol Cell Biol. 2004 Aug;24(15):6811-23. (PMID: 15254247)
Gene. 1999 Apr 16;230(2):145-54. (PMID: 10216252)
Plant Cell Physiol. 2000 Aug;41(8):920-31. (PMID: 11038052)
PLoS Genet. 2017 Mar 8;13(3):e1006663. (PMID: 28273088)
Plant Mol Biol. 2002 Dec;50(6):915-24. (PMID: 12516862)
Mol Cell. 2010 Oct 22;40(2):216-27. (PMID: 20965417)
Nature. 2017 Jul 13;547(7662):241-245. (PMID: 28636597)
Plant J. 1998 Dec;16(6):735-43. (PMID: 10069079)
Science. 2018 Jul 27;361(6400):. (PMID: 29930091)
Trends Genet. 2017 Aug;33(8):529-539. (PMID: 28647055)
Cell. 1983 Jun;33(2):509-18. (PMID: 6190573)
Curr Opin Plant Biol. 2004 Dec;7(6):614-20. (PMID: 15491908)
Biochemistry. 2018 May 1;57(17):2462-2469. (PMID: 29473743)
Nat Genet. 2004 Feb;36(2):162-6. (PMID: 14745447)
Plant Physiol. 2007 Jul;144(3):1587-97. (PMID: 17513485)
Nat Rev Mol Cell Biol. 2017 May;18(5):285-298. (PMID: 28225081)
Mol Cell. 2007 Jun 22;26(6):775-80. (PMID: 17588513)
Curr Opin Struct Biol. 2019 Oct;58:88-96. (PMID: 31252218)
PLoS Genet. 2013 Aug;9(8):e1003755. (PMID: 24009530)
Development. 2005 Sep;132(17):3963-76. (PMID: 16079157)
Proc Natl Acad Sci U S A. 2011 Feb 22;108(8):3430-5. (PMID: 21282611)
J Cell Biol. 2009 Jan 26;184(2):269-80. (PMID: 19171759)
Plant J. 2006 Nov;48(4):592-605. (PMID: 17059405)
Cell. 2018 Dec 13;175(7):1842-1855.e16. (PMID: 30449618)
Cell. 2002 Oct 18;111(2):197-208. (PMID: 12408864)
Nat Commun. 2020 Jan 14;11(1):270. (PMID: 31937751)
Nat Protoc. 2007;2(7):1565-72. (PMID: 17585298)
Science. 2018 Jul 27;361(6400):412-415. (PMID: 29930094)
Science. 2009 Jun 26;324(5935):1729-32. (PMID: 19460965)
Nat Genet. 2011 Jun 05;43(7):715-9. (PMID: 21642989)
Nature. 2019 May;569(7755):265-269. (PMID: 31043738)
Curr Biol. 2017 Oct 23;27(20):R1097-R1102. (PMID: 29065286)
Mol Cell. 2015 Oct 15;60(2):208-19. (PMID: 26412307)
Cell. 2019 Jan 24;176(3):419-434. (PMID: 30682370)
Cell. 2009 Feb 20;136(4):701-18. (PMID: 19239890)
Plant Cell. 2010 Jan;22(1):108-23. (PMID: 20061554)
J Cell Biol. 2000 Sep 4;150(5):949-62. (PMID: 10973987)
Nucleic Acids Res. 2018 Feb 28;46(4):1777-1792. (PMID: 29228330)
Bioinformatics. 2009 May 1;25(9):1105-11. (PMID: 19289445)
Cold Spring Harb Perspect Biol. 2010 Dec;2(12):a000711. (PMID: 21068152)
Annu Rev Cell Dev Biol. 2014;30:39-58. (PMID: 25288112)
Mol Cell. 2014 Apr 10;54(1):156-165. (PMID: 24725596)
Mol Biol Cell. 2004 Jul;15(7):3233-43. (PMID: 15133128)
Plant Cell. 2013 Oct;25(10):3657-83. (PMID: 24179125)
Front Plant Sci. 2018 Aug 15;9:1174. (PMID: 30158945)
Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11224-8. (PMID: 1454802)
Plant Cell. 2008 Feb;20(2):292-306. (PMID: 18296627)
Plant Cell. 2001 Nov;13(11):2471-81. (PMID: 11701882)
Exp Cell Res. 2008 Oct 15;314(17):3175-86. (PMID: 18674533)
Semin Cell Dev Biol. 2019 Aug;92:126-133. (PMID: 30974171)
Cell Commun Signal. 2016 Jan 05;14:1. (PMID: 26727894)
Reproduction. 2005 Oct;130(4):453-65. (PMID: 16183863)
Science. 2018 Jan 5;359(6371):. (PMID: 29301985)
EMBO J. 2013 Jul 17;32(14):2073-85. (PMID: 23778966)
Methods. 2001 Dec;25(4):402-8. (PMID: 11846609)
Plant Cell. 2016 Jan;28(1):55-73. (PMID: 26721863)
Cell. 2010 Nov 12;143(4):606-16. (PMID: 21074051)
Genes Dev. 2019 Jul 1;33(13-14):799-813. (PMID: 31171700)
J Exp Bot. 2019 Feb 5;70(3):739-745. (PMID: 30445526)
EMBO J. 2008 Apr 23;27(8):1277-88. (PMID: 18388858)
Mol Plant. 2014 Aug;7(8):1397-1401. (PMID: 24711291)
BMC Plant Biol. 2019 Oct 16;19(1):429. (PMID: 31619182)
Curr Opin Plant Biol. 2017 Feb;35:45-53. (PMID: 27866125)
Trends Biochem Sci. 2017 Jul;42(7):531-542. (PMID: 28483375)
Proc Natl Acad Sci U S A. 2017 Feb 28;114(9):2131-2136. (PMID: 28202730)
Sci Rep. 2019 Mar 1;9(1):3234. (PMID: 30824726)
EMBO J. 2018 Mar 1;37(5):. (PMID: 29438978)
Nat Cell Biol. 2015 Nov;17(11):1504-11. (PMID: 26458246)
Substance Nomenclature :
0 (Arabidopsis Proteins)
0 (At2g03150 protein, Arabidopsis)
0 (Calcium-Binding Proteins)
0 (Histones)
0 (Nuclear Proteins)
0 (RNA, Messenger)
K3Z4F929H6 (Lysine)
Entry Date(s) :
Date Created: 20200722 Date Completed: 20200824 Latest Revision: 20200826
Update Code :
20201023
PubMed Central ID :
PMC7413564
DOI :
10.1371/journal.pbio.3000782
PMID :
32692742
Czasopismo naukowe
Tight regulation of gene transcription and mRNA splicing is essential for plant growth and development. Here we demonstrate that a plant-specific protein, EMBRYO DEFECTIVE 1579 (EMB1579), controls multiple growth and developmental processes in Arabidopsis. We demonstrate that EMB1579 forms liquid-like condensates both in vitro and in vivo, and the formation of normal-sized EMB1579 condensates is crucial for its cellular functions. We found that some chromosomal and RNA-related proteins interact with EMB1579 compartments, and loss of function of EMB1579 affects global gene transcription and mRNA splicing. Using floral transition as a physiological process, we demonstrate that EMB1579 is involved in FLOWERING LOCUS C (FLC)-mediated repression of flowering. Interestingly, we found that EMB1579 physically interacts with a homologue of Drosophila nucleosome remodeling factor 55-kDa (p55) called MULTIPLE SUPPRESSOR OF IRA 4 (MSI4), which has been implicated in repressing the expression of FLC by forming a complex with DNA Damage Binding Protein 1 (DDB1) and Cullin 4 (CUL4). This complex, named CUL4-DDB1MSI4, physically associates with a CURLY LEAF (CLF)-containing Polycomb Repressive Complex 2 (CLF-PRC2). We further demonstrate that EMB1579 interacts with CUL4 and DDB1, and EMB1579 condensates can recruit and condense MSI4 and DDB1. Furthermore, emb1579 phenocopies msi4 in terms of the level of H3K27 trimethylation on FLC. This allows us to propose that EMB1579 condensates recruit and condense CUL4-DDB1MSI4 complex, which facilitates the interaction of CUL4-DDB1MSI4 with CLF-PRC2 and promotes the role of CLF-PRC2 in establishing and/or maintaining the level of H3K27 trimethylation on FLC. Thus, we report a new mechanism for regulating plant gene transcription, mRNA splicing, and growth and development.
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