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Tytuł pozycji:

The role of sodium thiocyanate supplementation during dextran sodium sulphate-stimulated experimental colitis.

Tytuł:
The role of sodium thiocyanate supplementation during dextran sodium sulphate-stimulated experimental colitis.
Autorzy:
Liu Y; Discipline of Pathology, Charles Perkins Centre, Faculty of Medicine and Health, The University of Sydney, NSW, 2006, Australia. Electronic address: .
Burton T; Animal Behavioural Facility, Charles Perkins Centre, School of Medical Sciences and the Bosch Institute, The University of Sydney, NSW, 2006, Australia. Electronic address: .
Rayner BS; Heart Research Institute, Sydney Medical School, The University of Sydney, NSW, 2006, Australia. Electronic address: .
San Gabriel PT; Discipline of Pathology, Charles Perkins Centre, Faculty of Medicine and Health, The University of Sydney, NSW, 2006, Australia. Electronic address: .
Shi H; Discipline of Pathology, Charles Perkins Centre, Faculty of Medicine and Health, The University of Sydney, NSW, 2006, Australia. Electronic address: .
El Kazzi M; Discipline of Pathology, Charles Perkins Centre, Faculty of Medicine and Health, The University of Sydney, NSW, 2006, Australia. Electronic address: .
Wang X; Discipline of Pathology, Charles Perkins Centre, Faculty of Medicine and Health, The University of Sydney, NSW, 2006, Australia. Electronic address: .
Dennis JM; Discipline of Pathology, Charles Perkins Centre, Faculty of Medicine and Health, The University of Sydney, NSW, 2006, Australia. Electronic address: .
Ahmad G; Discipline of Pathology, Charles Perkins Centre, Faculty of Medicine and Health, The University of Sydney, NSW, 2006, Australia. Electronic address: .
Schroder AL; Discipline of Pathology, Charles Perkins Centre, Faculty of Medicine and Health, The University of Sydney, NSW, 2006, Australia. Electronic address: .
Gao A; Discipline of Pathology, Charles Perkins Centre, Faculty of Medicine and Health, The University of Sydney, NSW, 2006, Australia. Electronic address: .
Witting PK; Discipline of Pathology, Charles Perkins Centre, Faculty of Medicine and Health, The University of Sydney, NSW, 2006, Australia. Electronic address: .
Chami B; Discipline of Pathology, Charles Perkins Centre, Faculty of Medicine and Health, The University of Sydney, NSW, 2006, Australia. Electronic address: .
Źródło:
Archives of biochemistry and biophysics [Arch Biochem Biophys] 2020 Oct 15; Vol. 692, pp. 108490. Date of Electronic Publication: 2020 Jul 25.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Publication: <2000- > : San Diego, CA : Elsevier
Original Publication: New York, NY : Academic Press
MeSH Terms:
Colitis*/chemically induced
Colitis*/drug therapy
Colitis*/enzymology
Colitis*/pathology
Colon*/enzymology
Colon*/pathology
Dextran Sulfate/*toxicity
Peroxidase/*metabolism
Thiocyanates/*pharmacology
Animals ; Disease Models, Animal ; Female ; Mice
Contributed Indexing:
Keywords: Inflammation; Myeloperoxidase; Thiocyanate; Ulcerative colitis
Substance Nomenclature:
0 (Thiocyanates)
5W0K9HKA05 (sodium thiocyanate)
9042-14-2 (Dextran Sulfate)
EC 1.11.1.7 (Peroxidase)
Entry Date(s):
Date Created: 20200730 Date Completed: 20201119 Latest Revision: 20201119
Update Code:
20240104
DOI:
10.1016/j.abb.2020.108490
PMID:
32721434
Czasopismo naukowe
Ulcerative colitis is a condition characterised by the infiltration of leukocytes into the gastrointestinal wall. Leukocyte-MPO catalyses hypochlorous acid (HOCl) and hypothiocyanous acid (HOSCN) formation from chloride (Cl - ) and thiocyanous (SCN - ) anions, respectively. While HOCl indiscriminately oxidises biomolecules, HOSCN primarily targets low-molecular weight protein thiols. Oxidative damage mediated by HOSCN may be reversible, potentially decreasing MPO-associated host tissue destruction. This study investigated the effect of SCN - supplementation in a model of acute colitis. Female mice were supplemented dextran sodium sulphate (DSS, 3% w/v) in the presence of 10 mM Cl - or SCN - in drinking water ad libitum, or with salts (NaCl and NaSCN only) or water only (controls). Behavioural studies showed mice tolerated NaSCN and NaCl-treated water with water-seeking frequency. Ion-exchange chromatography showed increased fecal and plasma SCN - levels in thiocyanate supplemented mice; plasma SCN - reached similar fold-increase for smokers. Overall there was no difference in weight loss and clinical score, mucin levels, crypt integrity and extent of cellular infiltration between DSS/SCN - and DSS/Cl - groups. Neutrophil recruitment remained unchanged in DSS-treated mice, as assessed by fecal calprotectin levels. Total thiol and tyrosine phosphatase activity remained unchanged between DSS/Cl - and DSS/SCN - groups, however, colonic tissue showed a trend in decreased 3-chlorotyrosine (1.5-fold reduction, p < 0.051) and marked increase in colonic GCLC, the rate-limiting enzyme in glutathione synthesis. These data suggest that SCN - administration can modulate MPO activity towards a HOSCN-specific pathway, however, this does not alter the development of colitis within a DSS murine model.
(Crown Copyright © 2020. Published by Elsevier Inc. All rights reserved.)

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