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Tytuł:
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Dual expression of transgenic delta-5 and delta-6 desaturase in tilapia alters gut microbiota and enhances resistance to Vibrio vulnificus infection.
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Autorzy:
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Chiang KY; Department of Life Science, National Taiwan University, Taipei, Taiwan.; Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei, Taiwan.
Lin WC; Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, Ilan, Taiwan.
Tsai TY; Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, Ilan, Taiwan.
Lin CW; Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei, Taiwan.
Huang SJ; Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei, Taiwan.
Huang CY; Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei, Taiwan.
Wu SH; Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan.
Ken CF; Institute of Biotechnology, National Changhua University of Education, Changhua, Taiwan.
Gong HY; Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan.; Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan.
Chen JY; Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, Ilan, Taiwan.
Wu JL; Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei, Taiwan.
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Źródło:
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PloS one [PLoS One] 2020 Jul 30; Vol. 15 (7), pp. e0236601. Date of Electronic Publication: 2020 Jul 30 (Print Publication: 2020).
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: San Francisco, CA : Public Library of Science
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MeSH Terms:
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Disease Resistance*/genetics
Gastrointestinal Microbiome*
Fatty Acid Desaturases/*metabolism
Fish Proteins/*metabolism
Linoleoyl-CoA Desaturase/*metabolism
Tilapia/*microbiology
Vibrio vulnificus/*pathogenicity
Animals ; Animals, Genetically Modified/genetics ; Animals, Genetically Modified/microbiology ; Delta-5 Fatty Acid Desaturase ; Diet/veterinary ; Discriminant Analysis ; Docosahexaenoic Acids/metabolism ; Fatty Acid Desaturases/genetics ; Fatty Acids, Omega-3/metabolism ; Fish Diseases/microbiology ; Fish Diseases/pathology ; Fish Proteins/genetics ; Gene Expression ; Least-Squares Analysis ; Linoleoyl-CoA Desaturase/genetics ; Tilapia/genetics ; Vibrio Infections/pathology ; Vibrio Infections/veterinary
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References:
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Substance Nomenclature:
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0 (Delta-5 Fatty Acid Desaturase)
0 (Fatty Acids, Omega-3)
0 (Fish Proteins)
25167-62-8 (Docosahexaenoic Acids)
EC 1.14.19.- (Fatty Acid Desaturases)
EC 1.14.19.3 (Linoleoyl-CoA Desaturase)
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SCR Disease Name:
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Vibrio vulnificus infection
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Entry Date(s):
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Date Created: 20200731 Date Completed: 20200929 Latest Revision: 20211204
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Update Code:
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20240104
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PubMed Central ID:
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PMC7392239
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DOI:
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10.1371/journal.pone.0236601
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PMID:
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32730353
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Omega-3 polyunsaturated fatty acids (n-3 PUFAs), such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), exhibit antibacterial and anti-inflammatory activities. Furthermore, diets rich in n-3 PUFAs are known to improve disease resistance and limit pathogen infection in commercial aquaculture fishes. In this study, we examined the effects of transgenic overexpression of n-3 PUFA biosynthesis genes on the physiological response to bacterial infection in tilapia. We first established tilapia strains with single or dual expression of salmon delta-5 desaturase and/or delta-6 desaturase and then challenged the fish with Vibrio vulnificus infection. Interestingly, our data suggest that n-3 PUFA-mediated alterations in gut microbiota may be important in determining disease outcome via effects on immune response of the host. Both liver- and muscle-specific single and dual expression of delta-5 desaturase and delta-6 desaturase resulted in higher n-3 PUFA content in transgenic fish fed with a LO basal diet. The enrichment of n-3 PUFAs in dual-transgenic fish is likely responsible for their improved survival rate and comparatively reduced expression of inflammation- and immune-associated genes after V. vulnificus infection. Gut microbiome analysis further revealed that dual-transgenic tilapia had high gut microbiota diversity, with low levels of inflammation-associated microbiota (i.e., Prevotellaceae). Thus, our findings indicate that dual expression of transgenic delta-5 and delta-6 desaturase in tilapia enhances disease resistance, an effect that is associated with increased levels of n-3 PUFAs and altered gut microbiota composition.
Competing Interests: The authors have declared that no competing interests exist.
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