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Tytuł:
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Identification of a Continuously Stable and Commercially Available Cell Line for the Identification of Infectious African Swine Fever Virus in Clinical Samples.
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Autorzy:
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Rai A; ARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USA.; Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN 37830, USA.
Pruitt S; ARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USA.; Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN 37830, USA.
Ramirez-Medina E; ARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USA.; Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN 37830, USA.
Vuono EA; ARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USA.; Department of Pathobiology and Population Medicine, Mississippi State University, P.O. Box 6100, Starkville, MS 39762, USA.
Silva E; ARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USA.; Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 66506, USA.
Velazquez-Salinas L; ARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USA.; Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 66506, USA.
Carrillo C; APHIS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USA.
Borca MV; ARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USA.
Gladue DP; ARS, USDA, Plum Island Animal Disease Center, Greenport, New York, NY 11944, USA.
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Źródło:
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Viruses [Viruses] 2020 Jul 28; Vol. 12 (8). Date of Electronic Publication: 2020 Jul 28.
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Typ publikacji:
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Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
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Język:
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English
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Imprint Name(s):
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Original Publication: Basel, Switzerland : MDPI
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MeSH Terms:
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African Swine Fever/*virology
African Swine Fever Virus/*isolation & purification
Disease Outbreaks/*veterinary
Epithelial Cells/*virology
African Swine Fever Virus/physiology ; Animals ; Cell Culture Techniques ; Cell Line ; Chlorocebus aethiops ; Macrophages/virology ; Sus scrofa/virology ; Swine
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References:
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Contributed Indexing:
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Keywords: ASF; ASFV; diagnostics
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Entry Date(s):
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Date Created: 20200801 Date Completed: 20210224 Latest Revision: 20210224
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Update Code:
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20240104
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PubMed Central ID:
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PMC7472077
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DOI:
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10.3390/v12080820
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PMID:
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32731642
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African swine fever virus (ASFV) is causing outbreaks both in domestic pigs and wild boar in Europe and Asia. In 2018, the largest pig producing country, China, reported its first outbreak of African swine fever (ASF). Since then, the disease has quickly spread to all provinces in China and to other countries in southeast Asia, and most recently to India. Outbreaks of the disease occur in Europe as far west as Poland, and one isolated outbreak has been reported in Belgium. The current outbreak strain is highly contagious and can cause a high degree of lethality in domestic pigs, leading to widespread and costly losses to the industry. Currently, detection of infectious ASFV in field clinical samples requires accessibility to primary swine macrophage cultures, which are infrequently available in most regional veterinary diagnostic laboratories. Here, we report the identification of a commercially available cell line, MA-104, as a suitable substrate for virus isolation of African swine fever virus.
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