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Tytuł pozycji:

In-cell architecture of an actively transcribing-translating expressome.

Tytuł :
In-cell architecture of an actively transcribing-translating expressome.
Autorzy :
O'Reilly FJ; Bioanalytics Unit, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany.
Xue L; Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany.; Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences, 69120 Heidelberg, Germany.
Graziadei A; Bioanalytics Unit, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany.
Sinn L; Bioanalytics Unit, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany.
Lenz S; Bioanalytics Unit, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany.
Tegunov D; Department of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, 37077 Göttingen, Germany.
Blötz C; Department of General Microbiology, Institute of Microbiology and Genetics, GZMB, Georg-August-University Göttingen, 37077 Göttingen, Germany.
Singh N; Department of General Microbiology, Institute of Microbiology and Genetics, GZMB, Georg-August-University Göttingen, 37077 Göttingen, Germany.
Hagen WJH; Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany.
Cramer P; Department of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, 37077 Göttingen, Germany.
Stülke J; Department of General Microbiology, Institute of Microbiology and Genetics, GZMB, Georg-August-University Göttingen, 37077 Göttingen, Germany.
Mahamid J; Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany. .
Rappsilber J; Bioanalytics Unit, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany. .; Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh, EH9 3BF, UK.
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Źródło :
Science (New York, N.Y.) [Science] 2020 Jul 31; Vol. 369 (6503), pp. 554-557.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
Język :
English
Imprint Name(s) :
Publication: : Washington, DC : American Association for the Advancement of Science
Original Publication: New York, N.Y. : [s.n.] 1880-
MeSH Terms :
Peptide Chain Elongation, Translational*
Protein Interaction Maps*
Transcription, Genetic*
Mycoplasma pneumoniae/*metabolism
Mycoplasma pneumoniae/*ultrastructure
Bacterial Proteins/metabolism ; DNA-Directed RNA Polymerases/metabolism ; Genome, Bacterial ; Humans ; Mycoplasma pneumoniae/genetics ; Peptide Elongation Factors/metabolism ; Ribosomes/metabolism ; Transcriptome
References :
Elife. 2017 Oct 13;6:. (PMID: 29027901)
Proc Natl Acad Sci U S A. 1985 Jul;82(14):4663-7. (PMID: 2991886)
Nat Protoc. 2016 Nov;11(11):2054-65. (PMID: 27685097)
J Struct Biol. 2015 May;190(2):143-54. (PMID: 25770733)
Int J Mol Sci. 2019 May 27;20(10):. (PMID: 31137816)
Elife. 2016 Oct 04;5:. (PMID: 27697152)
Mol Cell Proteomics. 2012 Mar;11(3):M111.014126. (PMID: 22286754)
Anal Chem. 2017 Apr 4;89(7):3829-3833. (PMID: 28267312)
Structure. 2015 Sep 1;23(9):1743-1753. (PMID: 26256537)
Mol Cell. 2017 Oct 19;68(2):388-397.e6. (PMID: 28988932)
RNA. 2019 May;25(5):600-606. (PMID: 30733327)
Nat Methods. 2017 Aug;14(8):793-796. (PMID: 28671674)
Curr Protoc Bioinformatics. 2016 Jun 20;54:5.6.1-5.6.37. (PMID: 27322406)
EMBO Rep. 2009 Sep;10(9):997-1002. (PMID: 19680289)
Biophys J. 2015 May 5;108(9):2097-102. (PMID: 25954868)
J Struct Biol. 2005 Mar;149(3):227-34. (PMID: 15721576)
Nucleic Acids Res. 2018 Jul 2;46(W1):W329-W337. (PMID: 29860432)
Structure. 2012 Dec 5;20(12):2003-13. (PMID: 23217682)
Nat Biotechnol. 2012 Oct;30(10):918-20. (PMID: 23051804)
J Struct Biol. 2007 Jan;157(1):38-46. (PMID: 16859925)
Nat Methods. 2018 Nov;15(11):955-961. (PMID: 30349041)
J Struct Biol. 2017 Feb;197(2):191-198. (PMID: 27313000)
J Struct Biol. 2009 Nov;168(2):352-6. (PMID: 19580872)
Science. 2017 Apr 14;356(6334):194-197. (PMID: 28408604)
J Comput Chem. 2004 Oct;25(13):1605-12. (PMID: 15264254)
Cell. 2018 Jun 14;173(7):1650-1662.e14. (PMID: 29887376)
Mol Cell. 2019 Jul 25;75(2):298-309.e4. (PMID: 31103420)
J Struct Biol. 2006 Nov;156(2):342-54. (PMID: 16875842)
J Struct Biol. 2014 Aug;187(2):174-186. (PMID: 24937760)
EMBO Rep. 2017 Feb;18(2):264-279. (PMID: 27974378)
Science. 2009 Nov 27;326(5957):1235-40. (PMID: 19965468)
Cell. 2009 Jan 23;136(2):261-71. (PMID: 19167328)
Science. 2009 Nov 27;326(5957):1268-71. (PMID: 19965477)
ACS Synth Biol. 2020 Jul 17;9(7):1693-1704. (PMID: 32502342)
Proc Natl Acad Sci U S A. 1998 May 12;95(10):5752-6. (PMID: 9576956)
Nat Rev Microbiol. 2011 May;9(5):319-29. (PMID: 21478900)
EMBO J. 2005 Oct 19;24(20):3576-87. (PMID: 16193062)
Mol Cell. 2019 Apr 4;74(1):143-157.e5. (PMID: 30795892)
J Bacteriol. 2004 Dec;186(23):7936-43. (PMID: 15547265)
FEMS Microbiol Rev. 2015 Jul;39(4):541-54. (PMID: 25878038)
J Struct Biol. 2011 Sep;175(3):288-99. (PMID: 21616153)
J Struct Biol. 2015 Mar;189(3):177-83. (PMID: 25661704)
PLoS Genet. 2012;8(3):e1002520. (PMID: 22412379)
Wiley Interdiscip Rev RNA. 2016 Sep;7(5):620-36. (PMID: 27117863)
Science. 2010 Apr 23;328(5977):501-4. (PMID: 20413501)
Nat Commun. 2015 Apr 23;6:6941. (PMID: 25903689)
Mol Cell Proteomics. 2014 Nov;13(11):2927-43. (PMID: 25161197)
Proc Natl Acad Sci U S A. 2013 Dec 24;110(52):20994-9. (PMID: 24324137)
Nucleic Acids Res. 2015 Jul 1;43(W1):W389-94. (PMID: 25883141)
Nat Struct Mol Biol. 2018 Nov;25(11):1000-1008. (PMID: 30374081)
Elife. 2018 Jun 01;7:. (PMID: 29856314)
J Struct Biol. 2005 Oct;152(1):36-51. (PMID: 16182563)
Nucleic Acids Res. 2019 Jan 8;47(D1):D607-D613. (PMID: 30476243)
PLoS Biol. 2012 Jan;10(1):e1001244. (PMID: 22272186)
Bioinformatics. 2009 May 1;25(9):1189-91. (PMID: 19151095)
Biophys J. 2017 Dec 5;113(11):2344-2353. (PMID: 29211988)
Nucleic Acids Res. 2019 Jul 2;47(W1):W636-W641. (PMID: 30976793)
Mol Syst Biol. 2012 Feb 28;8:571. (PMID: 22373819)
Elife. 2018 Nov 09;7:. (PMID: 30412051)
Genes Dev. 2000 Oct 15;14(20):2664-75. (PMID: 11040219)
Cell. 2017 Jun 15;169(7):1240-1248.e23. (PMID: 28622509)
Nucleic Acids Res. 2018 Jul 2;46(W1):W296-W303. (PMID: 29788355)
J Proteome Res. 2019 Mar 1;18(3):1363-1370. (PMID: 30693776)
J Struct Biol. 2012 May;178(2):177-88. (PMID: 22193517)
iScience. 2020 Aug 21;23(8):101352. (PMID: 32726726)
Mol Syst Biol. 2019 Sep;15(9):e8994. (PMID: 31556486)
Science. 2010 Apr 23;328(5977):504-8. (PMID: 20413502)
J Struct Biol. 2012 May;178(2):139-51. (PMID: 22245546)
Structure. 2011 Jul 13;19(7):945-54. (PMID: 21742261)
Nature. 2011 May 19;473(7347):337-42. (PMID: 21593866)
Curr Opin Microbiol. 2013 Apr;16(2):112-7. (PMID: 23433801)
EMBO J. 2010 Feb 17;29(4):717-26. (PMID: 20094031)
Mol Cell. 2009 Jan 16;33(1):97-108. (PMID: 19150431)
Nat Methods. 2019 Nov;16(11):1146-1152. (PMID: 31591575)
Trends Cell Biol. 2016 Nov;26(11):825-837. (PMID: 27671779)
Structure. 2019 Jan 2;27(1):175-188.e6. (PMID: 30393052)
Mol Cell. 2018 Mar 1;69(5):816-827.e4. (PMID: 29499136)
Mol Syst Biol. 2015 Jan 21;11(1):780. (PMID: 25609650)
Cell Syst. 2019 Aug 28;9(2):143-158.e13. (PMID: 31445891)
J Struct Biol. 1996 Jan-Feb;116(1):71-6. (PMID: 8742726)
Anal Chem. 2017 May 16;89(10):5311-5318. (PMID: 28402676)
Protein Sci. 2018 Jan;27(1):14-25. (PMID: 28710774)
PLoS Genet. 2014 May 08;10(5):e1004363. (PMID: 24809820)
Mol Syst Biol. 2017 Jul 25;13(7):936. (PMID: 28743795)
Nat Commun. 2017 Sep 20;8(1):629. (PMID: 28931821)
Nucleic Acids Res. 2011 Sep 1;39(17):7803-15. (PMID: 21652641)
J Struct Biol. 2012 Dec;180(3):519-30. (PMID: 23000701)
Grant Information :
203149 United Kingdom WT_ Wellcome Trust; 693023 International ERC_ European Research Council; International Deutsche Forschungsgemeins; 103139 United Kingdom WT_ Wellcome Trust; 760067 International ERC_ European Research Council
Substance Nomenclature :
0 (Bacterial Proteins)
0 (Peptide Elongation Factors)
EC 2.7.7.6 (DNA-Directed RNA Polymerases)
Entry Date(s) :
Date Created: 20200801 Date Completed: 20200914 Latest Revision: 20200914
Update Code :
20201023
PubMed Central ID :
PMC7115962
DOI :
10.1126/science.abb3758
PMID :
32732422
Czasopismo naukowe
Structural biology studies performed inside cells can capture molecular machines in action within their native context. In this work, we developed an integrative in-cell structural approach using the genome-reduced human pathogen Mycoplasma pneumoniae We combined whole-cell cross-linking mass spectrometry, cellular cryo-electron tomography, and integrative modeling to determine an in-cell architecture of a transcribing and translating expressome at subnanometer resolution. The expressome comprises RNA polymerase (RNAP), the ribosome, and the transcription elongation factors NusG and NusA. We pinpointed NusA at the interface between a NusG-bound elongating RNAP and the ribosome and propose that it can mediate transcription-translation coupling. Translation inhibition dissociated the expressome, whereas transcription inhibition stalled and rearranged it. Thus, the active expressome architecture requires both translation and transcription elongation within the cell.
(Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.)
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