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Tytuł pozycji:

GOLGA4, A Golgi matrix protein, is dispensable for spermatogenesis and male fertility in mice.

Tytuł:
GOLGA4, A Golgi matrix protein, is dispensable for spermatogenesis and male fertility in mice.
Autorzy:
Guo S; Institute Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China.
Chunyu Lv; Institute Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China.
Ouyang S; Institute Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China.
Wang X; Institute Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China.
Liao A; Institute Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China.
Yuan S; Institute Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China; Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen, Guangdong, 518057, China. Electronic address: .
Źródło:
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2020 Aug 27; Vol. 529 (3), pp. 642-646. Date of Electronic Publication: 2020 Jul 18.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Publication: <2002- >: San Diego, CA : Elsevier
Original Publication: New York, Academic Press.
MeSH Terms:
Autoantigens/*genetics
Fertility/*genetics
Gene Expression Profiling/*methods
Golgi Matrix Proteins/*genetics
Spermatogenesis/*genetics
Animals ; Autoantigens/metabolism ; Base Sequence ; Female ; Golgi Matrix Proteins/metabolism ; Humans ; Liver/metabolism ; Male ; Mice, Knockout ; Ovary/metabolism ; Stomach/chemistry ; Testis/metabolism
Contributed Indexing:
Keywords: CRISPR/Cas9; GOLGA4; Male fertility; Mice; Spermatogenesis
Substance Nomenclature:
0 (Autoantigens)
0 (Golga4 protein, mouse)
0 (Golgi Matrix Proteins)
Entry Date(s):
Date Created: 20200802 Date Completed: 20210222 Latest Revision: 20210222
Update Code:
20240104
DOI:
10.1016/j.bbrc.2020.05.170
PMID:
32736686
Czasopismo naukowe
During acrosome biogenesis, numerous granules formed from trans-Golgi stacks and accumulated in the concave region of the nuclear surface that is essential for acrosome formation. Several Golgi-associated proteins were involved in this process. However, the specific function of Golgi-associated proteins, especially Golgi matrix protein, during acrosome biogenesis remains elusive. In this study, we identified GOLGA4, as a Golgi matrix protein, highly expressed in mouse testes. To explore the function of GOLGA4 in spermatogenesis, we generated a Golga4 global knockout mouse line using CRISPR/Cas9 technology and demonstrated that Golga4 knockout males are fertile with normal morphology of testis and sperm. Furthermore, testicular histology showed no significant difference between WT and KO mice. Together, our data demonstrate that GOLGA4 is dispensable for mouse spermatogenesis and male fertility.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020. Published by Elsevier Inc.)

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